اثر بهبوددهنده الاژیک اسید بر اختلال حافظه ناشی از دیابت در مو‌ش‌های بزرگ آزمایشگاهی: نقش استرس اکسیداتیو

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران

چکیده

مقدمه و هدف: دیابت ملیتوس با اختلال حافظه همراه است. الاژیک اسید (EA) پلی فنولی طبیعی با اثرات ضددیابتی و حفاظت‌کننده عصبی است. هدف مطالعه حاضر بررسی اثر حفاظتی الاژیک اسید بر اختلال حافظه ناشی از دیابت و ارزیابی نقش اثر ضداسترس اکسیداتیو این ترکیب بود.
مواد و روش ها: موش‌های بزرگ آزمایشگاهی نر، نژاد ویستار به‌طور تصادفی به چهار گروه (n=8) کنترل، دیابتی، دیابتی دریافت‌کننده دوز 25 mg/kg EA  و دیابتی دریافت‌کننده دوز50 mg/kg  EA تقسیم شدند. دیابت به‌وسیله تزریق داخل صفاقی تک‌دوز 60 mg/kg از استرپتوزوتوسین (STZ) القا شد. پس از 4 هفته دریافت داخل معده‌ای (گاواژ) EA (یک بار در روز)، بررسی حافظه توسط تست حافظه احترازی غیرفعال (PAL) انجام شد. در انتها فاکتورهای مرتبط با استرس اکسیداتیو شامل مالون دی آلدئید (MDA) و ظرفیت آنتی‌اکسیدانی تام (TAC) در بافت هیپوکامپ که تحت بیهوشی عمیق از مغز حیوانات جدا شد ارزیابی شدند.
نتایج: حیوانات دیابتیک به‌صورت قابل توجه کاهش زمان تأخیر در ورود به قسمت تاریک (STLr)‌ و افزایش زمان باقیماندن در محفظه تاریک (TDC) را در تست PAL و همچنین افزایش MDA و کاهش TAC را در مقایسه با گروه کنترل نشان دادند. درمان موش‌های دیابتیک با الاژیک اسید به شکل معنادار سبب افزایش STLr ، کاهش TDC و افزایش TAC (به‌وسیله هر دو دوز EA) و کاهش MDA (به‌وسیله دوز50  mg/kg EA) شد.
نتیجه‌گیری: یافته‌ها اثر بهبوددهنده حافظه الاژیک اسید را در حیوانات دیابتی از طریق تضعیف استرس اکسیداتیو نشان دادند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Ameliorative effect of ellagic acid against diabetes-induced memory deficit in rats: the role of oxidative stress

نویسندگان [English]

  • Safoura Raoufi
  • Abdolrahman Sarihi
  • Alireza Komaki
  • Siamak Shahidi
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
چکیده [English]

Background and Objective: Diabetes mellitus is associated with memory impairment. Ellagic acid (EA) is a natural polyphenol with anti-diabetic and neuroprotective effects. The aim of the present study was to investigate the protective effect of ellagic acid on diabetes induced memory impairment and to evaluate the role of its antioxidant effect.
Materials and Methods: Male Wistar rats were randomly divided into four groups (n=8): control, diabetic, diabetic receiving 25 mg/kg of EA, and diabetic receiving 50 mg/kg of EA. Diabetes was induced by a single intraperitoneal injection of 60 mg/kg of streptozotocin (STZ). After receiving EA (once a day) by oral gavage for four weeks, passive avoidance learning (PAL) test was used to evaluate memory. Finally, oxidative stress related factors including malondialdehyde (MDA) and total antioxidant capacity (TAC) were evaluated in the hippocampal tissue separated from the animal brains under deep anesthesia.
Results: Diabetic animals showed marked reductions in step-through latency (STLr) and elevation in time spent in the dark compartment (TDC)  in the retention trial and elevation of MDA level and diminution of TAC compared to controls significantly. Treatment of diabetic rats with EA markedly increased STLr, decreased TDC and enhanced TAC (by both EA doses) and reduced MDA (at a dose of 50 mg/kg of EA).
Conclusion: The results displayed learning and memory improving effect of EA in diabetic rats via attenuating oxidative stress.

کلیدواژه‌ها [English]

  • Diabetes mellitus
  • Ellagic Acid
  • Memory
  • Oxidative stress
  • Streptozotocin

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دانشور پزشکی
دوره 31، شماره 3 - شماره پیاپی 166
مرداد و شهریور 1402
صفحه 1-13

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