تاثیر هشت هفته تمرین مقاومتی بر مقدار پروتئین KIF5B بافت عضله نعلی و بازکننده طویل انگشتان موش های بزرگ آزمایشگاهی نر سالمند

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه تربیت دبیر شهید رجائی، تهران، ایران

2 مرکز تحقیقات علوم اعصاب، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

چکیده

مقدمه و هدف: کاهش موتورپروتئین‌های مؤثر در انتقال آکسوپلاسمی به عنوان یک دلیل احتمالی در بروز سارکوپنیا مطرح می شود. هدف پژوهش حاضر، تعیین تاثیر هشت هفته تمرین مقاومتی بر مقدار پروتئین KIF5B بافت عضله نعلی و بازکننده طویل انگشتان در موش‌های بزرگ آزمایشگاهی نر سالمند بود.
مواد و روش ها: تعداد 18 سر موش نر سالمند و جوان به طور تصادفی در 3 گروه تمرین مقاومتی سالمند، شم تمرین سالمند و شم تمرین جوان تقسیم شدند. گروه تمرین مقاومتی سالمند برای هشت هفته، هر هفته 4 روز، به بالا رفتن از نردبان یک متری با یک نوبت و 8 تکرار و با فاصله استراحتی 2 دقیقه بین تکرار‌ها پرداختند. برای اندازه گیری مقدار پروتئین KIF5B از روش الایزا، برای اندازه گیری وزن نسبی عضلات از ترازوی وزن کشی و برای آزمون فرضیه ها از آزمون تحلیل واریانس یک راهه و آزمون تعقیبی توکی در سطح 05/0>p استفاده شد.
نتایج: به دنبال سالمندی مقدار پروتئین KIF5B، در عضله نعلی و بازکننده طویل انگشتان در مقایسه با گروه شم جوان به طور معناداری کاهش یافت. هشت هفته تمرین مقاومتی باعث افزایش معنادار مقدار پروتئین KIF5B در عضله نعلی و بازکننده طویل انگشتان نسبت به گروه شم تمرین سالمند شد. وزن نسبی هر دو عضله در گروه تمرین مقاومتی نسبت به شم تمرین سالمند افزایش معنادار داشت.
نتیجه‌گیری: هشت هفته تمرین مقاومتی باعث افزایش مقدار پروتئین KIF5B در عضله نعلی و بازکننده طویل انگشتان و همچنین هایپرتروفی آن ها در موش‌های بزرگ آزمایشگاهی نر سالمند می شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of eight weeks of resistance training on KIF5B protein in soleus and extensor digital longus muscle tissue in aged male rats

نویسندگان [English]

  • Mojtaba Sadegh Ghomi 1
  • Majid Kashef 1
  • Mojtaba Salehpour 1
  • Fariba Khodagholi 2
1 Department of Exercise Physiology, Faculty of Sport Science , Shahid Rajaee Teacher Training University, Tehran, Iran
2 Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran
چکیده [English]

Background and Objective: The reduction of effective motor proteins in axoplasmic transport is proposed as a possible reason for the occurrence of sarcopenia. The aim of the present study was determination of the effect of eight weeks of resistance training on amount of KIF5B protein in soleus and extensor digital longus (EDL) muscle tissue in aged male rats.
Materials and Methods: 18 Aged and young male rats were randomly divided into 3 groups: aged resistance training, aged sham exercise and young sham exercise. The aged resistance training group climbed a one-meter ladder for eight weeks, 4 days a week, with one set and 8 repetitions, with a 2-minute rest interval between repetitions. To measure the amount of KIF5B protein, ELISA method was used, to measure the relative weight of muscles, a weighing scale was used, and to test the hypotheses, one-way analysis of variance and Tukey's post hoc test were used at the level of p≤0.05.
Results: Following aging, the amount of KIF5B protein decreased significantly in soleus muscle and EDL compare to Young Sham group. Eight weeks of resistance training caused a significant increase in the amount of KIF5B protein in the soleus and EDL compared to the aged sham exercise. The Relative weight of both muscles in the resistance training group had a significant increase compared to the aged sham exercise.
Conclusion: Eight weeks of resistance training increases the amount of KIF5B protein in soleus and EDL muscles and their hypertrophy in aged male rats.

کلیدواژه‌ها [English]

  • Resistance training
  • KIF5B
  • Soleus muscle
  • Extensor digital longus muscle
  • Aged rat

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