اثر فلاونوئید کوئرستین بر پتانسیل غشای میتوکندری و انفیلتراسیون نوتروفیلی به دنبال آسیب بیضه القا شده توسط متوترکسات در موش‌ بزرگ آزمایشگاهی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران

2 دانشگاه شاهد

3 1. گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

4 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران

10.22070/daneshmed.2022.15392.1144

کلیدواژه‌ها


عنوان مقاله [English]

Effect of the flavonoid quercetin on mitochondrial membrane potential and neutrophilic infiltration against methotrexate-induced testicular injury in the rat

نویسندگان [English]

  • mehrdad roghani
  • e j 1
  • Manizheh Karami 2
  • f t 3
  • n h 4
1 گروه زیست شناسی، دانشکده علوم پایه، دانشگاه شاهد، تهران، ایران
2 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
3 1. گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران
4 گروه فیزیولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران
چکیده [English]

Background and Objective: Methotrexate as a chemotherapeutic drug causes damage and oxidative stress in testicular tissue. Quercetin is a flavonoid with antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the effect of quercetin flavonoid on mitochondrial membrane potential and neutrophil infiltration following testicular damage due to methotrexate in rats.
Materials and Methods: Rats were randomly divided into 5 groups: control, treated with quercetin, methotrexate and two groups of methotrexate treated with quercetin at doses of 10 and 50 mg/kg. Methotrexate was injected intraperitoneally at a dose of 20 mg/kg. Quercetin was administered daily by gavage from one day before methotrexate injection until two weeks later. At the end of the test, the testes were isolated and the activity of myeloperoxidase enzyme was measured as a specific marker of neutrophil infiltration and the potential of mitochondrial membrane in homogeneity of testicular tissue.
Results: Quercetin at a dose of 50 mg/kg could improve the mitochondrial membrane potential compared to the methotrexate group but could not show a significant effect on reducing neutrophil infiltration.
Conclusion: The results of this study briefly showed that quercetin treatment reduces some of the side effects of methotrexate in testicular tissue including adverse changes in mitochondrial membrane potential and neutrophilic infiltration.

کلیدواژه‌ها [English]

  • Quercetin
  • Mitochondrial membrane potential
  • Neutrophilic infiltration
  • Testis
  • Methotrexate