تاثیر تمرین استقامتی و تناوبی با شدت بالا بر محتوای پروتئین‌های mTOR، CRTC1 و CRTC2 در بافت چربی زیرجلدی موش‌های دیابتی نوع 1 و2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم ورزشی، مؤسسه‌ی آموزش عالی آپادانا، شیراز، ایران

2 گروه عمومی و پایه، واحد هشتگرد، دانشگاه آزاد اسلامی، هشتگرد، ایران

چکیده

مقدمه و هدف: عوامل سلولی و ملکولی زیادی در تنظیم سوخت‌و‌ساز بافت چربی دخیل هستند، که دیابت می‌تواند منجر به نقص عملکردی آن‌ها شود. بنابراین هدف از انجام تحقیق حاضر، تاثیر تمرین استقامتی و تناوبی با شدت بالا (HIIT) بر محتوای پروتئین‌های mTOR، CRTC1 و CRTC2 در بافت چربی زیرجلدی موش‌های دیابتی نوع 1 و 2 می‌باشد.
مواد و روش ها: در این مطالعه، 36 سر موش صحرایی نر 2 ماهه نر از نژاد اسپراگ‌داولی با میانگین وزن 30±280 گرم انتخاب ‏شدند. پس از القاء دیابت‌های نوع 1 (18 سر) و 2 (18 سر) از طریق محلول استرپتوزوتوسین و نیکوتین‌‌آمید، به روش تصادفی هر نوع دیابت به 3 گروه، تمرین استقامتی، HIIT و کنترل (هر گروه 6 سر) تقسیم ‏شدند. گروه‌های تمرینی 4 روز در هفته به‏‌مدت 4 هفته برنامه تمرینی استقامتی و HIIT را انجام دادند. محتوای متغیرهای تحقیق حاضر در بافت چربی زیرجلدی از طریق روش آزمایشگاهی وسترن بلات اندازه‌گیری شد. برای تجزیه‌وتحلیل داده‌ها از نرم‌افزار SPSS نسخه‌ی 23 و آزمون آماری آنوای یک‌طرفه و آزمون تعقیبی توکی استفاده‏ شد.
نتایج: تمرین استقامتی و HIIT منجر به افزایش معنی‌داری در محتوای پروتئین‌های mTOR (0001/0P=) و CRTC1  (0001/0P=) و CRTC2 (0001/0P=) در بافت چربی زیر جلدی شد.
نتیجه‌گیری: به نظر می‌رسد چهار هفته تمرین استقامتی و HIIT با افزایش پروتئین‌های تحقیق حاضر می‌تواند بافت چربی زیرجلدی را در آزمودنی‌های دیابتی نوع 1 و 2 تنظیم کند.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of endurance and high-intensity interval training on the content of mTOR, CRTC1 and CRTC2 proteins in subcutaneous adipose tissue of type 1 and 2 diabetic rats

نویسندگان [English]

  • Akbar Ghodratnama 1
  • Mohammad Sherafati Moghadam 1
  • Maryam Shabani 2
1 Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
2 Department of Pure and Basic Science, Hashtgerd Branch, Islamic Azad University, Hashtgerd, Iran
چکیده [English]

Background and Objective: Many cellular and molecular factors are involved in the regulation of adipose tissue metabolism, which diabetes can lead to their functional impairment. Therefore, the aim of the present study was to evaluate the effect of endurance and high-intensity interval training on the content of mTOR, CRTC1, and CRTC2 proteins in subcutaneous adipose tissue of type 1 and 2 diabetic rats.
Materials and Methods: In this study, 36 head of 2-month-old Sprague-Dawley male rats with a mean weight of 280±30 g were selected. After induction of type 1 (18 head) and 2 (18 head) diabetics through streptozotocin and nicotinamide solution, each type of diabetes was randomly divided into 3 groups: endurance training, HIIT, and control (6 heads per group). The training groups performed endurance training and HIIT program 4 days a week for 4 weeks. The content of the present study variables in subcutaneous adipose tissue was measured by in vitro Western blotting method. SPSS software version 23 and One-way ANOVA and Tukey post hoc tests were used to analyze the data.
Results: Endurance training and HIIT led to a significant increase in the protein content of mTOR (P=0.0001), CRTC1 (P=0.0001), and CRTC2 (P=0.0001) in subcutaneous adipose tissue.
Conclusion: It seems that four weeks of endurance training and HIIT can regulate subcutaneous adipose tissue in type 1 and type 2 diabetic subjects by increasing the proteins of the present study.

کلیدواژه‌ها [English]

  • CRTC
  • Diabetes
  • Endurance training
  • High-intensity interval training
  • mTOR

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دانشور پزشکی
دوره 30، شماره 2 - شماره پیاپی 159
خرداد و تیر 1401
صفحه 24-36

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