تاثیر هشت هفته تمرین ورزشی مقاومتی برBAX و BCL2 بافت هیپوکمپ موش های بزرگ آزمایشگاهی نر

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی دانشگاه تربیت دبیر شهید رجائی تهران، تهران، ایران

چکیده

مقدمه و هدف: آپوپتوز بافت عصبی خصوصاً هیپوکمپ، به عنوان یک عامل احتمالی در بروز بیماری های عصبی مانند بیماری آلزایمر شناخته شده است. شواهد نشان می دهند که فعالیت ورزشی، جنبه های گوناگونی از فعالیت سلول های عصبی را تحت تأثیر قرار می دهد و ممکن است که از مرگ سلول های عصبی جلوگیری نماید. هدف از تحقیق حاضر تعیین تأثیر هشت هفته تمرین ورزشی مقاومتی بر BAX و BCL2  بافت هیپوکمپ در موش های بزرگ آزمایشگاهی نر بود.
مواد و روش ها: بدین منظور تعداد 16 سر موش بزرگ آزمایشگاهی نر به طور تصادفی در 2 گروه هشت تایی (تمرین مقاومتی و کنترل) تقسیم شدند و گروه تمرین ورزشی مقاومتی برای هشت هفته و هر هفته سه روز با هشت تکرار و فواصل استراحتی دو دقیقه بین تکرارها با نردبان مقاومتی به طول یک متر و زاویه  85 درجه به فعالیت ورزشی پرداختند. برای اندازه گیری غلظت‌های BCL2 و BAX از روش الایزای ساندویچی و برای آزمون فرضیه ها از آزمون یومن ویتنی و آزمون ویلکاکسون استفاده شد.
نتایج: تفاوت معنی داری در میزان پروتئین BCL2 و BAX هیپوکمپ در گروه تمرین مقاومتی در مقایسه با گروه کنترل وجود داشت؛ به طوری که هشت هفته تمرین مقاومتی باعث افزایش معنی دار BCL2 نسبت به گروه کنترل شد، علاوه بر این هشت هفته تمرین مقاومتی باعث کاهش معنی دار BAX نسبت به گروه کنترل شد. بیشتر اینکه هشت هفته تمرین مقاومتی باعث کاهش معنی دار نسبت BAX به BCL2 نسبت به گروه کنترل شد (05/0P<).
نتیجه‌گیری: نتایج حاصل از این تحقیق نشان داد، به دلیل اینکه هشت هفته تمرین مقاومتی باعث افزایش معنی دار BCL2 و کاهش معنی دار BAX و نسبت BAX به BCL2 می شود می تواند باعث کاهش آپوپتوز سلول های بافت هیپوکمپ موش های بزرگ آزمایشگاهی نر شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of eight-week resistance training on BAX and BCL2 of hippocampus tissue in male rats

نویسندگان [English]

  • Majid Kashef
  • Mojtaba Salehpour
  • Fereshteh Shahidi
  • Mojtaba Sadegh Ghomi
Department of Exercise Physiology, Faculty of Sport Science, Shahid Rajaee Teacher training University, Tehran, Iran
چکیده [English]

Background and Objective: Nervous tissue apoptosis, especially of the hippocampus, has been identified as a potential contributor to neurological diseases such as Alzheimer's disease, and evidence suggests that exercise affects various aspects of nervous cell activity and may prevent the death of neurons cells. The aim of this study was to determine the effect of eight-week of resistance training on BAX and BCL2 in hippocampal tissue in male rats.
Materials and Methods: For this aim, 16 male rats were randomly divided into 2 groups of eight (resistance training  and control) and resistance training group exercised for 8 weeks and 3 days per weeks with eight frequency and 2 minutes rest between them with resistance ladder with 1 meter length and 85 degree angle. Sandwich Elisa method was used to measure BCL2 and BAX concentrations and U Mann-Whitney and Wilcoxon tests were used to test the hypotheses.
Results: There was significant difference in BCL2 and BAX of hippocampus tissue in compared to control group, so that eight week resistance training causes significant increase in BCL2 level in resistance exercise group as compared to control group. Also eight week resistance training causes significant decrease in BAX level of resistance exercise group as compared to control group. Furthermore, eight week resistance training cause significant decrease in BAX/BCL2 ratio in resistance groups as compared to control group (p<0.05).
Conclusion: The results of this study showed eight-week resistance training causes significant increase in BCL2 and significant decrease in BAX and BAX/BCL2 ratio. Thus, it can cause decrease hippocampus tissue apoptosis in male rats.

کلیدواژه‌ها [English]

  • Resistance training
  • Hippocampus
  • BCL2
  • bax
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