فلاونوئید مورین رفتارهای اضطرابی و افسردگی القاشده توسط پپتید آمیلوئید بتا را در موش‌های صحرایی کاهش می‌دهد

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان، ایران

2 گروه زیست‌شناسی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان، ایران

چکیده

مقدمه و هدف: مطالعات نشان داده است که قبل از بروز نقص حافظه در بیماری آلزایمر، اضطراب و افسردگی ظاهر می‌شود و مهار این اختلالات، احتمالاً از پیدایش و پیشرفت اختلال حافظه جلوگیری می‌کند. مورین نوعی فلاونول طبیعی است که دارای اثرات آنتی اکسیدانی، ضدالتهابی و محافظت کنندگی عصبی است. در این مطالعه، اثر مورین بر رفتارهای اضطرابی و افسردگی القاشده توسط پپتید آمیلوئید بتا در موش‌های بزرگ آزمایشگاهی بررسی شد.
مواد و روش ها: 40 سر موش بزرگ آزمایشگاهی نر نژاد ویستار به گروه‌های کنترل، مورین، آمیلوئید بتا و آمیلوئید بتا-مورین تقسیم شدند. آمیلوئید بتا 42-1 به صورت درون هیپوکامپی (هر طرف 4 میکروگرم) تزریق شد و مورین (20 mg/kg) نیز به صورت گاواژ به مدت 14 روز متوالی تیمار شد. در پایان دوره به ترتیب از ماز بعلاوه مرتفع و تست شنای اجباری برای بررسی رفتارهای اضطرابی و افسردگی استفاده شد.
نتایج: تزریق درون هیپوکامپی آمیلوئید بتا موجب کاهش معنی‌‌دار درصد ورود به بازوی باز و درصد زمان ماندن در بازوی باز در ماز بعلاوه مرتفع شد (001/0>P). مجموع زمان بی‌حرکتی این حیوانات نیز در تست شنای اجباری به طور چشمگیری افزایش یافت (001/0>P). مورین تا حد زیادی از بروز این تغییرات در موش‌های بزرگ آزمایشگاهی دریافت کننده آمیلوئید بتا ممانعت نمود.
نتیجه‌گیری: مورین از بروز رفتارهای اضطرابی و افسردگی در موش های بزرگ مدل بیماری آلزایمر جلوگیری می‌کند و احتمالاً اثرات محافظت کنندگی عصبی در برابر آمیلوئید بتا دارد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of flavonoid morin on anxiety and depression behaviors induced by amyloid-beta peptide in rats

نویسندگان [English]

  • Negin Mohammadi 1
  • Masoumeh Asle-Rousta 1
  • Mehdi Rahnema 1
  • Rahim Amini 2
1 Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
2 Department of Biology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
چکیده [English]

Background and Objective: Studies have shown that anxiety and depression appear before memory impairment in Alzheimer's disease and controlling these dysfunctions may prevent the onset and progression of memory deficit. Morin is a natural flavonol that has antioxidant, anti-inflammatory, and neuroprotective effects. In this study, we investigated the effect of morin on anxiety and depression behaviors induced by amyloid-beta peptide in rats.
Materials and Methods: Forty male Wistar rats were divided into control, morin, amyloid-beta, and amyloid-beta-morin groups. Amyloid-beta 1-42 was injected into the hippocampus (4 micrograms on each side) and morin (20 mg/kg) was treated by gavage for 14 consecutive days. At the end of the period,  elevated plus maze (EPM) and forced swimming tests were used to evaluate anxiety and depressive behaviors, respectively.
Results: Intrahippocampal injection of amyloid-beta significantly reduced the percentage of entry into the open arm and the percentage of time remaining in the open arm in the EPM (P < 0.001). Also, the total immobility time of these animals in the forced swimming test was significantly increased (P < 0.001). Morin largely prevented these changes in amyloid-beta injected rats.
Conclusion: Morin prevents anxiety behaviors and depression in Alzheimer's disease model rats and may have neuroprotective effects against amyloid-beta.

کلیدواژه‌ها [English]

  • Morin
  • Anxiety
  • Depression

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دانشور پزشکی
دوره 29، شماره 4 - شماره پیاپی 155
مهر و آبان 1400
صفحه 1-10

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