تاثیر 12 هفته تمرین استقامتی و مکمل ال آرژنین بر سطوح دی متیل آرژنین نامتقارن (ADMA) و نیتریک اکساید در زنان یائسه دارای پرفشار خونی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 دانشکده تربیت بدنی و علوم ورزشی، دانشگاه شهید بهشتی، تهران، ایران

چکیده

مقدمه و هدف: تمرین ورزشی و مداخلات تغذیه ای از قبیل مصرف ال آرژنین نقش موثری در کنترل پرفشار خونی دارند. بنابراین، در پژوهش حاضر تاثیر 12 هفته تمرین استقامتی و مکمل ال آرژنین بر سطوح دی متیل آرژنین نامتقارن (ADMA) و نیتریک اکساید (NO) در زنان مبتلا به پرفشار خونی مورد بررسی قرار گرفته است.
مواد و روش ها: 40 زن یائسه دارای پرفشار خونی (56/2±26/55 سال، شاخص توده بدن: 18/1±61/28 kg.m2) به صورت تصادفی در چهار گروه (10 نفری) شامل گروه های دارونما، ال آرژنین، تمرین و تمرین+ال آرژنین تقسیم شدند. برنامه تمرین استقامتی به مدت 12 هفته با 75-60 درصد ضربان قلب بیشینه اجرا شد. مصرف ال آرژنین نیز روزانه 6 گرم در نظر گرفته شد. خونگیری در دو مرحله پیش آزمون و پس آزمون (48 ساعت بعد از جلسه آخر تمرین یا مصرف ال آرژنین) انجام و سطوح ADMA و NO به روش الایزا اندازه گیری شد. تجزیه و تحلیل داده ها با نرم افزار SPSS24 و آزمون آنالیز کوواریانس انجام گردید.
نتایج: سطح ADMA در گروه تمرین و تمرین+ال آرژنین نسبت به گروه دارونما و ال آرژنین کاهش معناداری نشان داد (05/0>p). سطح NO در همه گروه ها در مقایسه با گروه دارونما به صورت معنادار افزایش یافت (05/0>p). با وجود کاهش فشار خون سیستولی در گروه  های ال آرژنین، تمرین و تمرین+ال آرژنین در مقایسه با گروه دارونما (001/0>p)، کاهش فشار خون سیستولی در گروه تمرین+ال آرژنین نسبت به گروه تمرین (001/0>p) و ال آرژنین (05/0>p) نیز معنادار بود.
نتیجه‌گیری: مصرف ال آرژنین اثرگذاری تمرین استقامتی در کاهش فشار خون سیستولی را افزایش می دهد که احتمالاً تاحدودی بواسطه افزایش بیشتر سطح NO اتفاق می افتد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of 12-weeks endurance training and L-arginine supplement on the levels of asymmetrical dimethylarginine (ADMA) and nitric oxide in postmenopausal hypertensive women

نویسندگان [English]

  • Bita Bordbarazari 1
  • Mandana Gholami 1
  • Khosrow Ebrahim 2
  • Farshad Ghazalian 1
  • Hossein Aabed Natanzi 1
1 Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Physical Education and Sports Sciences, Shahid Beheshti University, Tehran, Iran
چکیده [English]

Background and Objective: Exercise training and nutritional interventions such as L-arginine ingestion have an effective role in controlling hypertension. Therefore, the aim of present study was to investigate the effect of 12-weeks endurance training and L-arginine supplement on the levels of asymmetrical dimethylarginine (ADMA) and nitric oxide (NO) in hypertensive women.
Materials and Methods: 40 postmenopausal hypertensive women (55.26±2.56 years old, body mass index: 28.61±1.18 kg.m2) were randomly assigned in four groups (10 person) including: placebo, L-arginine, training and training+L-arginine groups. Endurance training program conducted for 12 weeks with 60-75 percent of maximum heart rate. L-arginine consumption considered 6 g daily. Blood sampling performed in pre and posttest (48 hours after last training session or L-arginine consumption) and ADMA and NO were measured by ELISA method. Data were analyzed by SPSS24 software and analysis of covariance test.
Results: ADMA in training and training+L-arginine groups indicated significant decrease compared to placebo and L-arginine groups (p < 0.05). NO levels significantly increased in all groups compared to placebo group (p < 0.05). Despite decrease in systolic blood pressure in L-arginine, training and training+L-arginine groups compared to placebo group (p < 0.001), decrease in systolic blood pressure in training+L-arginine group were significant compared to training (p < 0.001) and L-arginine (p=0.007) groups.
Conclusion: L-arginine consumption increases the effectiveness of endurance training in decreasing systolic blood pressure that is partly exerted by further increase in NO levels.

کلیدواژه‌ها [English]

  • Hypertension
  • Postmenopausal
  • Endurance training
  • L-arginine

مراجع

  1. Roush GC. Obesity-Induced Hypertension: Heavy on the Accelerator. Hypertension 2018; 71:116-135.
  2. Versari D, Daghini E, Virdis A, Ghiadoni L, Taddei S. Endothelial dysfunction as a target for prevention of cardiovascular disease. Diabetes Care 2009; 32(2): 314-321.
  3. Yilmaz MI, Sonmez A, Saglam M, Qureshi AR, Carrero JJ, Caglar K, et al. ADMA levels correlate with proteinuria, secondary amyloidosis, and endothelial dysfunction. Journal of the American Society of Nephrology 2008; 19(2):388-395.
  4. Sibal L, C Agarwal S, D Home P, H Boger R. The role of asymmetric dimethylarginine (ADMA) in endothelial dysfunction and cardiovascular disease. Current Cardiology Reviews 2010; 6(2):82-90.
  5. Alpoim PN, Sousa LP, Mota AP, Rios DR, Dusse LM. Asymmetric dimethylarginine (ADMA) in cardiovascular and renal disease. Clinica Chimica Acta 2015; 440:36-39.
  6. Perticone F, Sciacqua A, Maio R, Perticone M, Leone GG, Bruni R, et al. Endothelial dysfunction, ADMA and insulin resistance in essential hypertension. International Journal of Cardiology 2010; 142(3):236-241.
  7. Wang D, Strandgaard S, Iversen J, Wilcox CS. Asymmetric dimethylarginine, oxidative stress, and vascular nitric oxide synthase in essential hypertension. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 2009; 296(2):195-200.
  8. Wei D, He WY, Lv QZ. Effect of nisoldipine and olmesartan on endothelium‐dependent vasodilation in essential hypertensive patients. CNS Neuroscience & Therapeutics 2012; 18(5):400-5.
  9. Whelton SP, Chin A, Xin X, He J. Effect of aerobic exercise on blood pressure: a meta-analysis of randomized, controlled trials. Annals of Internal Medicine 2002; 136(7):493-503.
  10. Tsai JC, Yang HY, Wang WH, Hsieh MH, Chen PT, Kao CC, et al. The beneficial effect of regular endurance exercise training on blood pressure and quality of life in patients with hypertension. Clinical and Experimental Hypertension 2004; 26(3):255-65.
  11. Lekakis JP, Papathanassiou S, Papaioannou TG, Papamichael CM, Zakopoulos N, Kotsis V, et al. Oral L-arginine improves endothelial dysfunction in patients with essential hypertension. International Journal of Cardiology 2002; 86(2-3):317-23.
  12. Jablecka A, Bogdanski P, Balcer N, Cieslewicz A, Skoluda A, Musialik K. The effect of oral L-arginine supplementation on fasting glucose, HbA1c, nitric oxide and total antioxidant status in diabetic patients with atherosclerotic peripheral arterial disease of lower extremities. European Review for Medical and Pharmacological Sciences 2012; 16(3):342-50.
  13. Hambrecht R, Hilbrich L, Erbs S, Gielen S, Fiehn E, Schoene N, et al. Correction of endothelial dysfunction in chronic heart failure: additional effects of exercise training and oral L-arginine supplementation. Journal of the American College of Cardiology 2000; 35(3):706-13.
  14. Lucotti P, Setola E, Monti LD, Galluccio E, Costa S, Sandoli EP, et al. Beneficial effects of a long-term oral L-arginine treatment added to a hypocaloric diet and exercise training program in obese, insulin-resistant type 2 diabetic patients. American Journal of Physiology-Endocrinology and Metabolism 2006; 291(5): 906-12.
  15. Jahani M, Nabilpour M, Campillo RR. Effects of L-arginine Supplementation and Aerobic Training on Hemodynamic Indices of Obese Men. International Journal of Sport Studies for Health 2019; 2(1): e88017.
  16. Hansen AH, Nielsen JJ, Saltin B, Hellsten Y. Exercise training normalizes skeletal muscle vascular endothelial growth factor levels in patients with essential hypertension. Journal of Hypertension 2010; 28(6):1176-85.
  17. Mortensen SP, Nyberg M, Gliemann L, Thaning P, Saltin B, Hellsten Y. Exercise training modulates functional sympatholysis and α‐adrenergic vasoconstrictor responsiveness in hypertensive and normotensive individuals. The Journal of Physiology 2014; 592(14):3063-73.
  18. Ammar T. Effects of aerobic exercise on blood pressure and lipids in overweight hypertensive postmenopausal women. Journal of Exercise Rehabilitation 2015; 11(3):145-150.
  19. Dashtabi A, Mazloom Z, Fararouei M, Hejazi N. Oral L-arginine administration improves anthropometric and biochemical indices associated with cardiovascular diseases in obese patients: a randomized, single blind placebo controlled clinical trial. Research in Cardiovascular Medicine 2016; 5(1): e29419.
  20. Barkhidarian B, Khorshidi M, Shab-Bidar S, Hashemi B. Effects of L-citrulline supplementation on blood pressure: A systematic review and meta-analysis. Avicenna Journal of Phytomedicine 2019; 9(1):10-20.
  21. Khalaf D, Krüger M, Wehland M, Infanger M, Grimm D. The Effects of Oral l-Arginine and l-Citrulline Supplementation on Blood Pressure. Nutrients 2019;11(7):1679.
  22. Dong JY, Qin LQ, Zhang Z, Zhao Y, Wang J, Arigoni F, et al. Effect of oral L-arginine supplementation on blood pressure: a meta-analysis of randomized, double-blind, placebo-controlled trials. American Heart Journal 2011; 162(6):959-65.
  23. Nyberg M, Jensen LG, Thaning P, Hellsten Y, Mortensen SP. Role of nitric oxide and prostanoids in the regulation of leg blood flow and blood pressure in humans with essential hypertension: effect of high‐intensity aerobic training. The Journal of Physiology 2012; 590(6):1481-94.
  24. Larsen MK, Matchkov VV. Hypertension and physical exercise: the role of oxidative stress. Medicina 2016; 52(1):19-27.
  25. Campbell PT, Gross MD, Potter JD, Schmitz KH, Duggan C, McTiernan A, Ulrich CM. Effect of exercise on oxidative stress: a 12-month randomized, controlled trial. Medicine and Science in Sports and Exercise 2010; 42(8):1448.
  26. Kolluru GK, Sinha S, Majumder S, Muley A, Siamwala JH, Gupta R, et al. Shear stress promotes nitric oxide production in endothelial cells by sub-cellular delocalization of eNOS: A basis for shear stress mediated angiogenesis. Nitric Oxide 2010; 22(4):304-15.
  27. Tinken TM, Thijssen DH, Hopkins N, Dawson EA, Cable NT, Green DJ. Shear stress mediates endothelial adaptations to exercise training in humans. Hypertension 2010; 55(2):312-8.
  28. Lackland DT, Voeks JH. Metabolic syndrome and hypertension: regular exercise as part of lifestyle management. Current Hypertension Reports 2014; 16(11):492.
  29. Beck DT, Casey DP, Martin JS, Emerson BD, Braith RW. Exercise training improves endothelial function in young prehypertensives. Experimental Biology and Medicine 2013; 238(4):433-41.
  30. Di Francescomarino S, Sciartilli A, Di Valerio V, Di Baldassarre A, Gallina S. The effect of physical exercise on endothelial function. Sports Medicine 2009; 39(10):797-812.

  31. 116
     
    Urbich C, Fritzenwanger M, Zeiher AM, Dimmeler S. Laminar shear stress upregulates the complement-inhibitory protein clusterin: a novel potent defense mechanism against complement-induced endothelial cell activation. Circulation 2000 1;101(4):352-5.
  32. Cornelissen VA, Smart NA. Exercise training for blood pressure: a systematic review and meta‐analysis. Journal of the American Heart Association 2013; 2(1): e004473.
  33. Dimeo F, Pagonas N, Seibert F, Arndt R, Zidek W, Westhoff TH. Aerobic exercise reduces blood pressure in resistant hypertension. Hypertension 2012; 60(3):653-8.
  34. Richter B, Niessner A, Penka M, Grdić M, Steiner S, Strasser B, et al. Endurance training reduces circulating asymmetric dimethylarginine and myeloperoxidase levels in persons at risk of coronary events. Thrombosis and Haemostasis 2005; 94(12):1306-11.
  35. Jarrete AP, Novais IP, Nunes HA, Puga GM, Delbin MA, Zanesco A. Influence of aerobic exercise training on cardiovascular and endocrine-inflammatory biomarkers in hypertensive postmenopausal women. Journal of Clinical & Translational Endocrinology 2014; 1(3):108-14.
  36. Şumnu A, Cebeci E, Öztürk S, Gürsu M, Kasapoğlu E, Özkan O, et al. Relationship of ADMA Levels with Cardiovascular Parameters in Patients with Peritoneal Dialysis: A Bioimpedance Analysis Study. Journal of Academic Research in Medicine 2018; 8(3): 147-52.
  37. Palloshi A, Fragasso G, Piatti P, Monti LD, Setola E, Valsecchi G, et al. Effect of oral L-arginine on blood pressure and symptoms and endothelial function in patients with systemic hypertension, positive exercise tests, and normal coronary arteries. The American Journal of Cardiology 2004; 93(7):933-5.
  38. Martina V, Masha A, Gigliardi VR, Brocato L, Manzato E, Berchio A, et al. Long-term N-acetylcysteine and L-arginine administration reduces endothelial activation and systolic blood pressure in hypertensive patients with type 2 diabetes. Diabetes Care 2008;31(5):940-4.
  39. Boger RH, Ron ES. L-Arginine improves vascular function by overcoming deleterious effects of ADMA, a novel cardiovascular risk factor. Alternative Medicine Review 2005; 10(1):14-23.
دانشور پزشکی
دوره 29، شماره 3 - شماره پیاپی 154
مرداد و شهریور 1400
صفحه 104-117

فایل ها

هم رسانی

ارجاع به این مقاله

آمار