تاثیر چهار هفته تمرین استقامتی به همراه مکمل یاری پروبیوتیک بر بیان ژن Bax و Bcl-2 در کاردیومیوسیت رت های دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه الزهرا، تهران، ایران

چکیده

مقدمه و هدف: افزایش آپوپتوز کاردیومیوسیت ها از عوامل اصلی ایجاد کاردیومیوپاتی دیابتی است. با توجه به نقش پروبیوتیک ها و تمرینات ورزشی در بهبود عوارض ناشی از دیابت، هدف از پژوهش حاضر بررسی تاثیر هم افزای تمرین استقامتی و مکمل پروبیوتیک بر بیان ژن های Bax و Bcl-2 در کاردیومیوسیت های رت های دیابتی بود.
مواد و روش ها: 30 سر رت نر نژاد ویستار با میانگین وزن 10±270 گرم، به صورت تصادفی به 5 گروه: کنترل سالم (NC)، کنترل دیابتی (DC)، مکمل پروبیوتیک دیابتی (SDC)، تمرین دیابتی (TD) و مکمل پروبیوتیک - تمرین دیابتی (STD) تقسیم شدند. گروه های تمرینی با شدت60-65 درصد vVO2peak، 5 روز در هفته به مدت 4 هفته بر روی تردمیل دویدند. همزمان گروه های مکمل، روزانه 2 گرم مکمل پروبیوتیک دریافت کردند. بیان ژن های Bax و Bcl-2 در بطن چپ رت ها به روش qReal-Time PCR، و تجزیه و تحلیل داده ها با آزمون آنالیز واریانس دوطرفه با سطح معنی داری 05/0>p انجام شد.
نتایج: بیان ژنBax  در همه گروه ها نسبت به گروه DC کاهش داشت (05/0<p) اما در گروه TD نسبت به STD تفاوت معنادار نبود ( 918/0=p). همچنین بیان ژن Bcl-2 در همه  گروه ها نسبت به گروه DC افزایش یافت (001/0=p) اما در گروه  TD نسبت به STD این افزایش معنادارتر بود (002/0=p).
نتیجه‌گیری: به نظر می رسد تمرین استقامتی به همراه مکمل یاری پروبیوتیک با تاثیر هم افزا بر برخی عوامل موثر بر آپوپتوز کاردیومیوسیت ها می تواند در کاهش عوارض ناشی از دیابت در کاردیومیوسیت رت های دیابتی موثر باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of four-week endurance training with probiotic supplementation on the expression of Bax and Bcl-2 in cardiomyocytes of diabetic rats

نویسندگان [English]

  • Maryam Delfan
  • Niloofar Afrasiabian
Department of Exercise Physiology, Faculty of Sport Sciences, Alzahra University, Tehran, Iran
چکیده [English]

Background and Objective: Increased cardiomyocyte apoptosis is one of the main causes of diabetic cardiomyopathy. Considering the role of probiotics and exercise in improving the complications of diabetes, the aim of this study was to investigate the synergistic effect of endurance training and probiotic supplementation on the expression of Bax and Bcl-2 genes in cardiomyocytes of diabetic rats.
Materials and Methods: 30 male Wistar rats with an average weight of 270 ±10 g were randomly divided into 5 groups: Normal Control (NC), Diabetic Control (DC), Diabetic Probiotic Supplement (SDC), Diabetic Training (TD), and Probiotic Supplement. Probiotic-Diabetic Training (STD). Training groups with an intensity of 60-65% VO2peak ran on a treadmill in 5 days a week and for 4 weeks. At the same time, the supplement groups received 2 grams of the probiotic supplement daily. Expression of Bax and Bcl-2 genes in the left ventricle of rats was studied by qReal-Time PCR and data analysis was performed by two-way analysis of variance with a significance level of p  
Results: Bax gene expression was decreased in all groups compared to the DC group (p <0.05) but in the TD group there was no significant difference compared to the STD (p = 0.918). Also, Bcl-2 gene expression increased in all groups compared to the DC group (p = 0.001) but in the TD group, this increase was more significant than STD (p = 0.002).
 Conclusion: It seems that endurance training with probiotic supplementation with a synergistic effect on some factors affecting cardiomyocyte apoptosis can be effective in reducing the complications of diabetes in cardiomyocytes of diabetic rats.

کلیدواژه‌ها [English]

  • bax
  • bcl-2
  • Diabetes
  • Endurance training
  • Probiotics
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