تأثیر تمرین تناوبی با شدت بالا بر محتوای پروتئین‌های‌ میوستاتین و SMAD2/3 در بافت بطن چپ عضله قلب موش‌های صحرایی دیابتی نوع 1

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران.

2 ﮔﺮوه ﻋﻠﻮم ورزﺷﯽ، ﻣﻮﺳﺴﻪ آﻣﻮزش ﻋﺎﻟﯽ آﭘﺎداﻧﺎ، ﺷﯿﺮاز، اﯾﺮان

3 گروه علوم ورزشی، دانشکده‌ی علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران

چکیده

< p>مقدمه و هدف: اختلال در مسیر میوستاتین/SMAD می‌تواند منجر به نقص قلبی شود. دیابت می‌تواند در این مسیر اختلال ایجاد کند؛ بنابراین هدف از انجام تحقیق حاضر، تأثیر شش هفته تمرین تناوبی با شدت بالا (HIIT) بر محتوای پروتئین‌های میوستاتین و SMAD2/3 در بافت بطن چپ عضله قلب موش‌های صحرایی دیابتی نوع 1 می‌باشد.
مواد و روش ها: در این مطالعه، 12 سر موش صحرایی نر 2 ماهه نر از نژاد اسپراگ‌داولی با میانگین وزن 20±300 گرم انتخاب ‏شدند. پس از القاء دیابت از طریق محلول استرپتوزوتوسین، به روش تصادفی به 2 گروه، تمرین HIIT دیابتی (6 سر) و کنترل دیابتی (6 سر) تقسیم ‏شدند؛ گروه‌های تمرینی 4 روز در هفته به‏ مدت 6 هفته برنامه تمرینی را شامل 5 وهله 4 دقیقه‌ای با شدت معادل 85 تا 95 درصد حداکثر سرعت و دوره‌های استراحت فعال 3 دقیقه‌ای با شدت معادل 50 تا 60 درصد حداکثر سرعت انجام دادند؛ برای تجزیه‌وتحلیل داده‌ها از آزمون آماری t-مستقل استفاده‏ شد.
نتایج: شش هفته تمرین HIIT، منجر به کاهش معنی‌داری در محتوای پروتئین میوستاتین بین گروه‌های تمرین و کنترل در بافت عضله قلبی شد (044/0P=). همچنین، تمرین HIIT منجر به کاهش معنی‌داری در محتوای پروتئین SMAD2/3 بین گروه‌ تمرین و کنترل در بافت عضله قلبی شد (001/0P=).
نتیجه‌گیری: می‌توان گفت تمرین HIIT با کاهش محتوای پروتئین‌های میوستاتین و SMAD2/3 در بطن چپ قلبی توانسته است از آتروفی و نقص قلبی در آزمودنی‌های دیابتی نوع 1 جلوگیری کند. این کاهش می‌تواند منجر به هیپرتروفی فیزیولوژیک قلبی شود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of high intensity interval training on myostatin and SMAD2/3 proteins in the left ventricular tissue of the heart muscle of type 1 diabetic rats

نویسندگان [English]

  • Masoud Jokar 1
  • Mohammad Sherafati Moghadam 2
  • Mousa Amirahmadi 3
1 Department of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
2 2. Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
3 Department of Sport Sciences, School of Education and Psychology, University of Shiraz, Shiraz, Iran
چکیده [English]

< p >Background and Objective: Disruption of the myostatin/SMAD pathway can lead to heart failure. Diabetes can disrupte this pathway. Therefore, the present study aimed to evaluate the effect of six weeks of high-intensity interval training HIIT (on myostatin and SMAD2/3 proteins in the left ventricular tissue of the heart muscle of type 1 diabetic rats.
Materials and Methods: In this study, 12 Spragodoli male rats (2-month old) with a mean weight of 300±20 g were selected.  Through streptozotocin solution, they were randomly divided into 2 groups: diabetic endurance training (6 heads) and diabetic control (6 heads); The training groups performed the training program 4 days a week for 6 weeks, including 5 interval 4-minute with an intensity of 85 to 95% of the maximum speed, and 3-minute active rest periods with an intensity of 50 to 60% of the maximum speed; Independent t-test was used to analyze the data.
Results: Six weeks of HIIT training lead to a significant reduction in myostatin protein content between training and control groups in myocardial tissue (P=0.044). Also, HIIT training resulted in a significant decrease in SMAD2/3 protein content between training and control groups in myocardial tissue (P=0.001).
Conclusion: It can be said that HIIT training by reducing the content of myostatin and SMAD2/3 proteins in the left ventricle of the heart has been able to prevent cardiac atrophy in type 1 diabetic subjects. This reduction can lead to physiological cardiac hypertrophy.
Keywords: Cardiac muscle, High intensity interval training, Myostatin 

کلیدواژه‌ها [English]

  • Cardiac Muscle
  • High intensity interval training
  • Myostatin Protein
  • SMAD2/3 Protein
  • Type 1 Diabetes
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