تأثیر یک دوره تمرین تناوبی با شدت بالا بر محتوای پروتئین‌های AMPK و PGC-1α در بافت عضله قلب موش‌های صحرایی مبتلا به دیابت نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران

2 واحد هشتگرد، دانشگاه آزاد اسلامی، البرز، ایران

3 گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه شیراز، شیراز، ایران

چکیده

مقدمه و هدف: پروتئین‌های AMPK و PGC-1α از عوامل مهم در تنظیم فرآیندهایی مانند بایوژنز میتوکندری، حفظ تعادل انرژی داخل سلولی هستند که بیماری دیابت می‌تواند در عملکردهای این پروتئین‌ها اختلال ایجاد کند. هدف از این مطالعه، بررسی تأثیر یک دوره تمرین تناوبی با شدت بالا بر محتوای پروتئین‌های AMPK و PGC-1α در بافت عضله قلب موش‌های صحرایی نر مبتلا به دیابت نوع 2 می‌باشد.
مواد و روش‌ها: در این مطالعه تجربی، 12 سر موش صحرایی نر 2 ماهه از نژاد اسپراگوداولی با میانگین وزنی 20±270 گرم انتخاب و پس از دیابتی شدن از طریق القاء استرپتوزوتوسین و نیکوتین‌آمید به روش تصادفی به 2 گروه، تمرین و کنترل (هر گروه 6 سر) تقسیم ‏شدند. گروه تمرین 4 روز در هفته به‏ مدت 8 هفته به تمرین HIIT مطابق با برنامه تمرینی پرداختند؛ در حالی که گروه کنترل هیچ‌گونه برنامه تمرینی نداشتند. همچنین موش‌های صحرایی هیچ‌گونه درمانی با انسولین را در طول دوره پژوهش نداشتند. برای تجزیه‌ و تحلیل داده‌ها از آزمون تی مستقل استفاده‏ شد.
نتایج: افزایش معنی‌داری در محتوای پروتئین‌های AMPK (001/0P=) و PGC-1α (0001/0P=) در گروه‌ تمرین HIIT نسبت به کنترل مشاهده شد.
نتیجه‌گیری: نتایج نشان دادند که تمرین HIIT با افزایش محتوای پروتئین‌های AMPK و PGC-1α می‌تواند در افزایش ATP و همچنین افزایش بیوژنز میتوکندیریایی تاثیرگذار باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of a period of high-intensity interval training on the content of AMPK and PGC-1α proteins in the heart muscle tissue of rats with type 2 diabetes

نویسندگان [English]

  • Masoud Jokar 1
  • Mohammad Sherafati Moghadam 2
  • Farhad Daryanoosh 3
1 Department Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
2 Hashtgerd Branch, Islamic Azad University, Alborz, Iran
3 Department of Exercise Physiology, School of Education and Psychology, University of Shiraz, Shiraz, Iran
چکیده [English]

Background and Objective: AMPK and PGC-1α proteins are important factors in regulating processes such as mitochondrial biogenesis, maintaining intracellular energy balance, which diabetes can disturb the function of these proteins. The aim of this study was to investigate the effect of a period high-intensity interval training on the content of AMPK and PGC-1α proteins in the heart muscle tissue of male rats with type 2 diabetes.
Materials and Methods: In this experimental study, 12 two-month-old Sprague-Dawley rats with a mean weight of 270±20 g were selected. After diabetic induction with STZ and nicotinamide, rats were randomly assigned to two groups, training and control (6 rats in group each). The training group trained for 4 days a week in accordance with the training program for 8 weeks; while the control group did not have any training program. Also, rats did not receive any insulin treatment during the study period. The independent t-test was used to analyze the data. Significance level is considered p≤0.05.
Results: A significant increase was observed in the content of AMPK (P=0.001) and PGC-1α (P=0.0001) proteins in the High-Intensity Interval Training group compared to control.
Conclusion: The results showed that HIIT training could increase ATP and mitochondrial biogenesis by increasing the content of AMPK and PGC-1α proteins.

کلیدواژه‌ها [English]

  • Cardiac Muscle
  • High-Intensity interval training
  • AMPK
  • PGC-1α
  • Type 2 Diabetes
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