تأثیر تمرینات هوازی و اینتروال همراه با مصرف مکمل آستاگزانتین بر بیومارکرهای قلبی و مقاومت انسولینی در موش صحرایی نر دیابتی نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزشی, دانشکده تربیت بدنی, دانشگاه ارومیه, ارومیه, ایران

2 گروه فیزیولوژی, دانشکده پزشکی, دانشگاه علوم پزشکی ارومیه, ارومیه, ایران

10.22070/daneshmed.2020.3053

چکیده

مقدمه و هدف: با توسعه چشمگیر دیابت محصولات طبیعی همانند آنتی‌اکسیدان‌ها بطور گسترده‌ای برای کاهش پیشرفت عوارض دیابت به ‌کار برده می شوند. مطالعه حاضر با هدف بررسی اثر همزمان دو شیوه تمرین هوازی و اینتروال همراه با مکمل استاگزانتین بر سطوح بیومارکرهای قلبی و مقاومت انسولینی رت های دیابتی نوع 2 انجام شد.
مواد و روش ها: 35 سر موش صحرایی نر نژاد ویستار پس از القای دیابت بطور تصادفی در 7 گروه شامل دیابتی کنترل, دیابتی شم, دیابت+هوازی+مکمل, دیابت+اینتروال+مکمل, دیابت+اینتروال, دیابت+هوازی, دیابت+مکمل قرار گرفتند. تمرینات اینتروال به مدت 8 هفته با 5 جلسه در هفته با شدت 80 درصد Vo2max و تمرینات هوازی با شدت 65 تا 75 درصد Vo2max روی تردمیل اجرا شد. گروه های مکمل بعد از هر جلسه تمرینی روزانه 3 میلی‌گرم آستاگزانتین بصورت گاواژ دریافت کردند. سطوح سرمی پپتید ناتریورتیک نوع پرو B آمینوترمینال و تروپونین قلبی نوع T با الایزا و مقاومت انسولینی با HOMA-IR اندازه گیری شد و برای تجزیه وتحلیل داده ها آزمون آنالیز واریانس یک راهه بکاربرده شد.
نتایج: سطوح NTproBNP و CTnT بعد از هشت هفته ترکیب تمرین هوازی و اینتروال همراه با مصرف مکمل در مقایسه با گروه کنترل اختلاف معنی‌داری نشان داد (05/0>P) اما تفاوت ها نسبت به گروه مکمل و هردو شیوه تمرینی معنی‌دار نبود (05/0<P). مقاومت انسولینی نیز در همه گروه‌ها به غیر از گروه شم تفاوت معنی داری را نسبت به گروه کنترل نشان داد (05/0>P).
نتیجه‌گیری: نتایج نشان داد ترکیب هر دو مداخله تمرین و مکمل ممکن است بواسطه اثرات آنتی‌اکسیدانی خود با کاهش آسیب استرس اکسیداتیو از آسیب کاردیومیوپاتی دیابتی پیشگیری کنند اما با اثر تقویتی مضاعف همراه نبود.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of aerobic and interval training with the astaxanthin supplementation on cardiac biomarkers and insulin resistance in type 2 diabetic rats

نویسندگان [English]

  • Nasrin Ebadi 1
  • Mohammad Reza Zolfaghari 1
  • Firouz Ghaderi Pakdel 2
1 Department of Exercise Physiology, Faculty of Sport Sciences, Urmia University, Urmia, Iran
2 Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
چکیده [English]

Background and Objective: With the development of diabetes, natural products such as antioxidants are widely used to reduce the progression of diabetes. Therefore, the present study aimed to investigate the simultaneous effect of two exercise methods with astaxanthin supplement on the levels of cardiac biomarkers and insulin resistance of type 2 diabetic rats.
Materials and Methods: 35 Wistar male rats were randomly divided into seven groups after induction of diabetes, including diabetic control, diabetes sham, diabetes+aerobic exercise+supplement, diabetes+ interval exercise+supplement, diabetes+interval, diabetes+aerobic, diabetes+supplement were included. Interval training for eight weeks with five sessions per week with an intensity of 80% vo2max and aerobic training with an intensity of about 65 to 75% Vo2max was performed on the treadmill. Supplementary groups received 3 mg of astaxanthin daily by gavage after each training session. Serum levels of NtproBNP and CTnT were measured with ELISA and insulin resistance was measured with HOMA-IR. One-way analysis of variance was used to analyze of the data.
Results: Levels of NtproBNP and CTnT showed significant difference after eight weeks of combination of aerobic and interval training with supplementation as compared to the control group (P <0.05), but the differences compared to the supplementary group and both training methods were not significant (P >0.05). Insulin resistance in all groups except sham group showed a significant difference as compared to the control group (P <0.05).
Conclusion: The results showed that the combination of both exercise and supplement interventions may prevent diabetic cardiomyopathy due to their antioxidant effects by reducing oxidative stress damage, but was not associated with a dual strengthening effect.

کلیدواژه‌ها [English]

  • Exercise
  • Rat
  • Diabetes
  • astaxanthin
  • NTproBNP
  • CTnT
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