تأثیر تمرین مقاومتی بر روی عملکرد بیضه و فرآیند اسپرماتوژنز و پارامتر های اسپرمی موش های نر ویستار بالغ

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه فیزیولوژی ورزش، دانشکده انسانی، دانشگاه شاهد، تهران، ایران

2 مرکز تحقیقات نوروفیزیولوژی، دانشگاه شاهد، تهران، ایران

10.22070/daneshmed.2020.3052

چکیده

مقدمه و هدف: اختلال اسپرماتوژنز موجب ایجاد ناباروری در جنس نر می شود. هدف از پژوهش حاضر بررسی تأثیر تمرین مقاومتی بر روی عملکرد بیضه و فرآیند اسپرماتوژنز و پارامترهای اسپرمی موش های نر ویستار بالغ می باشد.
مواد و روش ها: تعداد 20 سر موش بالغ نر به ‌صورت تصادفی به 2 گروه 10 تایی، کنترل و تمرین مقاومتی تقسیم شدند. تمرین به مدت (پنج هفته و سه روز در هفته) با 50 درصد توده ی بدنی و 30 گرم اضافه بار با 90 ثانیه استراحت انجام شد. میزان تستوسترون سرم، سلول لیدیگ، تراکم چربی بینابینی بیضه، شاخص های اسپرماتوژنز و پارامتر های اسپرمی با روش آماری تفاوت بین گروهی با آزمون t-test با سطح (05/0>p) ارزیابی شد.
نتایج: تمرین مقاومتی باعث افزایش معنی دار سلول لیدیگ و تراکم چربی بینابینی بیضه نسبت به گروه کنترل شد (05/0>p)، ولی تأثیر معنی داری بر میزان تستوسترون سرم نداشت. آنالیز بافتی نشان داد که تمرین مقاومتی شاخص های اسپرماتوژنز (TDI شاخص تمایز لوله ای، SI شاخص اسپرمیوژنز و RI شاخص تجمعی) را نسبت به گروه کنترل کاهش می دهد (05/0>p)، اما تأثیر معنی-داری بر روی ارتفاع اپیتلیوم، تعداد و قطر لوله اسپرم ساز ندارد. بررسی پارامتر های اسپرمی نشان داد که تمرین مقاومتی موجب کاهش تعداد تحرک و زیست پذیری اسپرم و افزایش شکستگی (DNA) می شود (05/0>p)، اما تأثیر معنی داری بر بلوغ اسپرم ندارد.
نتیجه‌گیری: تمرین مقاومتی باعث ایجاد اختلال در فرآیند اسپرماتوژنز و پارامتر های اسپرمی شده و می تواند تأثیر منفی در سیستم باروری مردان داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of resistance training on testicular function and spermatogenesis process and sperm parameters of adult male Wistar rats

نویسندگان [English]

  • Milad Khosravi Sadr 1
  • Esmail Nasiri 1
  • Mohsen Khalili 2
1 Department of Exercise Physiology, Faculty of Humanities, Shahed University, Tehran, Iran
2 Neurophysiology Research Center, Shahed University, Tehran, Iran
چکیده [English]

Background and Objective: It has been shown that spermatogenesis disorder could induce infertility in male individuals. This study investigated the effect of resistance training on testicular function, spermatogenesis and sperm parameters in adult male Wistar rats.
Materials and Methods: Twenty adult male rats were selected and randomly divided into two groups; 1- control (n=10) and resistance training (n=10). The exercise was performed three days a week for a period of 5 weeks. We chose the method of 50% of body mass with 30 g overload and a resting time of 90 s. Testosterone of the serum, interstitial testicular fat density, spermatogenesis indexes and other sperm parameters were measured and then analyzed by statistical t-test.
Results: In resistance training groups, there was a significant increase in the number of Leydig cells and interstitial fat density of the testis in comparison with the control group (p < 0.05), but this difference was not shown for the level of serum testosterone. Also, histological analysis showed that resistance training could significantly reduce spermatogenesis indices i.e. tubular differentiation index (TDI), spermiogenesis index (SI) and repopulation index (RI) in comparison with control group (p < 0.05). Nevertheless, this training could not yield the marked changes on number and the diameter of spermatozoon tube and its epitheliums. Finally, our results showed that motility and viability of sperm decrease prominently but, the amount of DNA fracture increased.
Conclusion: Resistance training could disrupt the process of spermatogenesis and sperm parameters that might yield infertility in male individuals.

کلیدواژه‌ها [English]

  • Resistance training
  • Spermiogenesis
  • Sperm parameters
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