تاثیر هشت هفته تمرینات پرشدت تناوبی بر بیان ژن های PGC-1α و ERRα دربافت چربی زیرجلدی رت های نر ویستار

نوع مقاله: مقاله پژوهشی

نویسندگان

گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه پیام نور، کرج، ایران

چکیده

مقدمه و هدف: PGC-1α و ERRα با همکاری هم برای رونویسی ژن‌های درگیر در متابولیسم انرژی عمل می کنند و در بیان و کنترل ژن های متابولیک نقش مهمی دارند. نقش تمرینات ورزشی بر بیان این دو ژن در بافت چربی کمتر شناخته شده است. لذا هدف پژوهش حاضر بررسی آثار تمرینات تناوبی با شدت بالا (HIIT) بر بیان ژن های PGC-1α و ERRα در بافت چربی زیرجلدی رت های نر ویستار بود.
مواد و روش ها: در مطالعه تجربی حاضر، تعداد 16 سر رت نر به دو گروه مساوی HIIT و کنترل تقسیم شدند. گروه HIIT به مدت ۸ هفته در برنامه های تمرینی شرکت کردند. گروه کنترل در هیچ برنامه تمرینی شرکت نکردند. پروتکل HIIT شامل ۴ مرحله دویدن در 4 دقیقه با شدت ۹۰ تا ۱۰۰ درصد VO2max و ۴ مرحله دویدن در 3 دقیقه در ۵۰ تا ۶۰ درصد VO2max بود. 24 ساعت پس از آخرین جلسه تمرین، بافت چربی زیر جلدی برداشته شد. روش RT-PCR برای اندازه گیری بیان ژن ها استفاده گردید. تحلیل داده ها با آزمون t مستقل و u مان ویتنی در سطح معنا داری 05/0≥P انجام شد.
نتایج: در پروتکل HIIT در مقایسه با گروه کنترل، در بیان هردو ژن PGC-1α و ERRαافزایش معنی دار مشاهده شد (001/0≤ P).
نتیجه‌گیری: نتایج نشان داد که تمرینات HIIT اثربخشی زیادی در بیان ژن های درگیر در متابولیسم انرژی دارد و لذا پیشنهاد می شود این شیوه تمرینی مورد توجه بیشتری قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of 8-week of high intensity interval training on PGC-1α and ERRα gene expression in subcutaneous adipose tissue of male Wistar rats

نویسندگان [English]

  • Shadi Fayyazi
  • Mohammadreza Asad
  • Saeed Naghibi
  • Mohammad Shariatzade
Faculty of Physical Education, Department of Sport Physiology, PNU, Karaj, Iran
چکیده [English]

Background and Objective: PGC-1α and ERRα work together to transcribe genes involved in energy metabolism and play an important role in the expression and control of metabolic genes. The role of exercise in the expression of these two genes in adipose tissue is less known. Therefore, this study aimed to investigate the effects of high intensity interval training (HIIT) on the expression of PGC-1α and ERRα genes in the subcutaneous adipose tissue of male Wistar rats.
Materials and Methods: In the experimental study, 16 male rats were divided into two equal groups of HIIT and control. HIIT group participated in training programs for 8 weeks. The control group did not participate in the training program. The HIIT protocol consisted of 4 stages of running in 4 minutes with intensity of 90 to 100% VO2max and 4 stages of running in 3 minutes in 50 to 60% of VO2max. 24 hours after the last training session, the subcutaneous adipose tissue was removed. The RT-PCR method was used to measure gene expression. Data analysis was performed with independent t-test and Mann-Whitney U at the significance level of P < 0.05.
Results: In the HIIT protocol, a significant increase was observed in the expression of both PGC-1α and ERRα genes in comparison with the control group (P < 0.001).
Conclusion: The results showed that HIIT training is very effective in expressing the genes involved in energy metabolism. Therefore, it is recommended that this training method be given more attention.

کلیدواژه‌ها [English]

  • Subcutaneous adipose tissue
  • HIIT
  • PGC-1α
  • ERRα
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