تأثیر تمرین تناوبی با شدت بالا بر مسیر کمپلکس یک هدف راپامایسین در پستانداران (mTORC1) در عضله اسکلتی خم‌کننده طویل انگشتان پا (FHL) موش‌های صحرایی دیابتی شده توسط استرپتوزوتوسین

نویسندگان

گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روانشناسی، گروه علوم ورزشی، دانشگاه شیراز، شیراز، ایران

چکیده

مقدمه و هدف: شناخته‌شده‌ترین مکانیسم تنظیم‌کننده فعالیت مسیر mTORC1 در عضله اسکلتی مسیر وابسته به انسولین/IGF-1 است. هنوز نقش تمرین HIIT بر این مسیر مهم در سنتز پروتئین در افراد مبتلا به دیابت نوع 2 بررسی نشده است. هدف از مطالعه حاضر، بررسی تأثیر تمرین تناوبی با شدت بالا بر مسیر کمپلکس یک هدف راپامایسین در پستانداران (mTORC1) در بافت عضله FHL موش‌های صحرایی مبتلا به دیابت نوع 2 است.
 
مواد و روش‌ها: در این مطالعه تجربی، 16 سر موش صحرایی نر 2 ماهه از نژاد اسپراگوداولی با میانگین وزن 20±260 گرم انتخاب و پس از دیابتی شدن از طریق القاء STZ و نیکوتین‌آمید به روش تصادفی به 2 گروه، تمرین (8 سر) و کنترل (8 سر) تقسیم ‏شدند؛ گروه تمرینی 4 روز در هفته مطابق با برنامه تمرینی به‏ مدت 4 هفته به فعالیت ورزشی پرداختند؛ در حالی که گروه کنترل هیچ‌گونه برنامه تمرینی نداشتند. همچنین موش‌های صحرایی هیچ‌گونه درمانی با انسولین را در طول دوره پژوهش نداشتند. برای تجزیه‌وتحلیل داده‌ها از آزمون t-مستقل استفاده‏ شد.
 
نتایج: افزایش معناداری در محتوای پروتئین‌های AKT1 (005/0p<)، mTOR (0001/0p<)، P70S6K1 (008/0p<) و 4E-BP1 (001/0p<) در گروه‌ تمرین نسبت به کنترل مشاهده شد.
 
نتیجه‌گیری: تمرین HIIT منجر به افزایش محتوای پروتئین‌های AKT1، mTOR، P70S6K1 و 4EBP1 در بافت عضله اسکلتی FHL آزمودنی‌های دیابتی تحقیق حاضر شد؛ بنابراین، با توجه به نقش‌های مهم این پروتئین‌ها در سنتز پروتئین، فعالیت ورزشی HIIT می‌تواند یک مکانیسم مهم برای افزایش سنتز پروتئین یا هیپرتروفی عضلانی باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of high intensity interval training on complex mammalian target of Rapamycin 1 (mTORC1) pathway in Flexor hallucis longus muscle (FHL) of streptozotocin-induced diabetic rats

نویسندگان [English]

  • Mohammad Sherafati Moghadam
  • Farhad Daryanoosh
  • Mohsen Salesi
  • Aliasghar Fallahi
  • Mohammad Hemati Nafar
چکیده [English]

Background and Objective: The most well-known mechanism for regulating complex mammalian target of rapamycin 1 (mTORC1) pathway activity is the insulin/IGF-1-dependent pathway in skeletal muscles. The role of high intensity interval training (HIIT) exercise has not yet been studied on this important pathway in protein synthesis among people with type 2 diabetes. The purpose of the present study was to investigate the effects of HIIT on mTORC1 pathway in Flexor hallucis longus muscle (FHL) of streptozotocin (STZ)-induced diabetic rats.
 
Materials and Methods: In this experimental study, sixty male Sprague–Dawley rats with a weight of 260±20 g were randomly assigned into two equal groups including control group (n= 8) and HIIT trained group (n=8) after inducing diabetes in them by STZ and nicotinamide. The latter group of rats were trained in accordance with the training program for 4 weeks (4 sessions per week). Control groups received no exercise intervention. Furthermore, rats did not receive any insulin therapy during the study period. Independent t-test was used to assess the difference between the groups. Differences were considered significant at p<0.05.
 
Results: There was a significant increase in the value of AKT1 (p<0.005), mTORC1 (p<0.0001), P70S6K1 (p<0.008) and 4E-BP1 (p<0.001) proteins in the HIIT trained group as compared to the control group.
 
Conclusion: Given the increase in the content of these proteins (AKT1, mTORC1, P70S6K1 and 4E-BP1) in the FHL skeletal muscle tissue of type 2 diabetic subjects and their important role in protein synthesis, HIIT's exercise may be considered as an important approach for increasing protein synthesis or muscle hypertrophy in these individuals.

کلیدواژه‌ها [English]

  • High Intensity Interval Training (HIIT)
  • mTORC1 signaling pathway
  • FHL skeletal muscle
  • Type 2 Diabetes
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