نویسندگان
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Background and Objective : Bacteriophage vectors recently have been considered as a gene transfer and vaccine delivery vehicles chiefly due to their low cost, safety, and physical stability. Since, little is known about phage mediated gene transfer in mammalian hosts, A group of invitro experiments were performed to ascertain gene transfercapability of these vehicles . Materials and Methods : For this purpose, different genes eg. (EGFP as positive control and PBR322 as negative control) were sub cloned into the vehicle Lambda ZAP cassettePossessing expression capability in animal cells.New Recombinant-phage constructs using packaged using bacteriophage lysates. In order to evaluate functionality of the produced bacterio phages EGFP bacteriophage particles were added to eukaryotic cell cultures and after 36 hours, amplified and purified phage particles were assessed for gene expression criteria in cell culture models. Results and Conclusion: The observation of fluorescent signals arising from GFP(green fluorescent protein) means vehicle expression in eukaryotic systems.stability of the recombinant phages as oral vaccine vehicles were checkedIn similar conditionsas in gastrointestinal tract. (Ph &destructing enzymes) The results of stability assay showed that the phages have potential for employing as oral vaccine vehicles.
کلیدواژهها [English]
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