The Effect of Eight Weeks of Resistance Training With and Without Stanozolol Injection on Histopathological Changes in the Prostate Tissue of Male Wistar Rats


Abstract
Background and Objective: Anabolic-androgenic steroids (AAS) and resistance training are thought to significantly influence the structure and function of the reproductive system, particularly the prostate gland. This study aimed to investigate the effects of eight weeks of resistance training, with and without Stanozolol administration, on histopathological alterations in the prostate tissue of male Wistar rats.
Materials and Methods:In this experimental study, 28 male Wistar rats (age: eight weeks; weight: 250 ± 25 g) were randomly assigned to four groups (n=7 per group): Resistance Training (RT), Control+Placebo (CN), Stanozolol administration (ST), and Resistance Training + Stanozolol (RT+ST). The RT protocol was performed intermittently over eight weeks(alternating between 4 and 3 sessions per week). Stanozolol was administered once weekly injections (5mg/kg body weight), while the CN and RT groups received saline. Data were analyzed using one-way ANOVA and the Kruskal-Wallis test, with the significance level set at (P<0.05).

Results: The results showed the RT+ST group exhibited a significantly greater epithelial height compared to the control group (P<0.05); however, no significant differences in epithelial height were observed among the other groups. Histopathological evaluation revealed that the ST and RT+ST groups showed significant increases in hyperplasia, pyknosis, vacuolar degeneration, and collagen deposition compared to the control group (P<0.05). Furthermore, hyperplasia was significantly higher in the RT group than in the control group(P<0.05).