The effect of aerobic training along with melatonin consumption on caspase-1, NLRP3, and IL-1β gene expression related to pyroptosis pathway in the liver tissue of type 1 diabetes male rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sport Sciences, Shoushtar Branch, Islamic Azad University, Shoushtar,Iran

2 Department of Physical Education and Sport Sciences, Masjed-Soleiman Branch, Islamic Azad University, Masjed-Soleiman,Iran

3 Department of Sport Sciences, School of Education and Psychology, Shiraz university, Shiraz, Iran

Abstract

Background and Objective: The programmed cell death pyroptosis is modulated and regulated by inflammatory stimuli and multiple cellular pathways. On the other hand, there is an interaction between diabetes as an inflammatory disease and the pyroptosis pathway. Therefore, the purpose of the research was to investigate the effect of aerobic training along with melatonin consumption on the pyroptosis signaling pathway in the liver tissue of streptozotocin-induced type 1 diabetes rats.
Materials and Methods In this experimental study, 30 eight-week-old male Wistar rats weighing 180±20 grams were randomly selected and 24 of them were made diabetic by injecting streptozotocin solution to induce type 1 diabetes. Type 1 diabetes rats were divided into 4 groups including diabetes control (DC), diabetes melatonin (DMel), diabetes aerobic training (DEx), diabetes aerobic training+melatonin (DEx+Mel). A healthy control group (HC) was also considered. The aerobic exercise program lasted six weeks, five days a week, and each session lasted 60 minutes. Variables were measured through Real-Time PCR laboratory method. The data were analyzed through one-way analysis of variance and Tukey's post-hoc statistical tests in SPSS version 29 software. The significance level was considered P≤0.05.
Results: A significant decrease in caspase-1 and NLRP3 levels was observed following six weeks of aerobic training and melatonin administration (P=0.001). Also IL-1β levels showed a significant decrease (P=0.002).
Conclusion: Reducing these factors can be an important strategy and approach to modulate the pyroptosis pathway by reducing inflammation in the liver tissue of diabetic subjects.

Keywords

References

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Daneshvar Medicine
Volume 32, Issue 6 - Serial Number 175
March and April 2025
Pages 14-28

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