Daneshvar Medicine

Daneshvar Medicine

The effect of endurance training and remote ischemic preconditioning on myocardial Beclin-1 gene expression in diabetic rats

Document Type : Original Article

Authors
Fazeleh Akbarnia 1
Amin Farzaneh Hesari 1
Abdollah Hashemvarzi 1
Aliasghar Farsavian 2
1 Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran
2 Department of Cardiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
10.22070/daneshmed.2024.19695.1554
Abstract
Background and Objective: Despite the protective effect of ischemic preconditioning (IPC) from tissues, its effect on diabetes-induced excessive autophagy is not clear. Autophagy could be inhibited or activated through exercise. The present study aimed to investigate the effect of endurance training and remote IPC on myocardial Beclin-1 gene expression in diabetic rats.
Materials and Method: In this experimental research, 35 Wistar rats were randomly divided into seven groups: normal control (C), diabetic (D), diabetic one leg ischemia (OI), diabetic two legs ischemia (TI), diabetic endurance training (E), diabetes one leg ischemia + endurance training (OIE), diabetes two legs ischemia+ endurance training (TIE). IPC was included three 5-minute cycles of ischemia, followed by five minutes of reperfusion. The endurance training groups performed exercise training for six weeks and five days a week and was included running on a treadmill. The speed for the first week is set at 9 meters per minute for 15 minutes. By the sixth week, the training speed was increased to 18 meters per minute for 30 minutes. Beclin-1 gene expression was measured by RT-PCR. Data were analyzed by one-way analysis of variance and Tukey post hoc tests.
Results: Beclin-1 gene expression significantly increased in D group compared to C group (p=0.0001). Becli-1 gene expression significantly decreased in OI (p=0.0001), TI (p=0.0001), E (p=0.0001), OIE (p=0.0001) and TIE (p=0.0001) compared to the D.
Conclusion: It seems that the effect of ischemia preconditioning in decreasing Beclin-1 is independent of the ischemia-affected muscle mass. Endurance training with IPC is more effective in decreasing Beclin-1 in diabetes than any of the exercise and IPC interventions alone.
Keywords
Exercise training
Ischemic preconditioning
Autophagy
Beclin-1
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Daneshvar Medicine
Volume 32, Issue 5 - Serial Number 174
January and February 2025
Pages 34-43