Daneshvar Medicine

Daneshvar Medicine

The effect of resistance training on the content of proteins associated with endoplasmic reticulum stress (PERK and NRF2) in the left ventricle of rats with type 2 diabetes

Document Type : Original Article

Authors
Fatemeh Solhdoust 1
Hamed Alizadeh Pahlavani 2
Mohammad Sherafati Moghadam 1
1 Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
2 Department of Physical Education, Farhangian University, Tehran, Iran
10.22070/daneshmed.2024.19505.1528
Abstract
Background and Objective: In recent years, resistance training has been proposed as a powerful intervention to improve the health of cardiovascular function in people with type 2 diabetes. Therefore, the aim of this study is the effect of resistance training on the content of endoplasmic reticulum stress-related proteins (PERK and NRF2) in the left ventricle of rats with type 2 diabetes.
Materials and Methods: In this experimental study, 12 2-month-old male Sprague Dawley rats with an average weight of 280±20 grams were selected. Type 2 diabetes was induced by injecting nicotinamide and streptozotocin solutions. Rats were randomly divided into 2 groups, diabetic resistance training and diabetic control. Resistance training consisted of 8 weeks and 3 sessions per week of climbing a vertical ladder with an 85 degree slope, one meter long with 26 steps and 2 cm space between each step. Western blot method was used to measure PERK and NRF2 protein content. Independent t-test was used for data analysis and the significance level was P≤0.05.
Results: After 8 weeks of resistance training, the content of PERK and NRF2 proteins showed a significant increase compared to the control group in the left ventricle of the heart of diabetic rats (P=0.01 and P=0.002, respectively).
Conclusion: It seems that resistance training can be useful for the heart health of subjects with type 2 diabetes and can be included in the rehabilitation guidelines of health professionals.
Keywords
Resistance exercise
Endoplasmic reticulum
Left ventricle
PERK protein
NRF2 protein
Type 2 diabetes
Subjects
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Daneshvar Medicine
Volume 32, Issue 4 - Serial Number 173
November and December 2024
Pages 36-46