Daneshvar Medicine

Daneshvar Medicine

Evaluation of the effect of eight weeks of aerobic exercise on ATP-binding cassette transporter mRNA expression in PBMC cells in middle-aged post-menopausal women

Document Type : Original Article

Authors
Akhtar Ghorghi
Habib Asgharpour
Reza Rezaei Shirazi
Department of Physical Education & Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran
10.22070/daneshmed.2024.19419.1522
Abstract
Background and Objective: Atherosclerosis is one of the most common causes of death in different societies, which occurs as a result of atherosclerosis of the coronary arteries and this complication is exacerbated in some stages of life, one of which is menopause. At this stage, due to reduced physiological estrogen levels and android obesity, increased activity of the sympathetic system malfunction and fat oxidation, endothelial dysfunction and vascular inflammation risk of cardiovascular disease in postmenopausal women increases. One of the factors reducing the risk of atherosclerosis is the expression of ATP-binding cassette transporter (ABCA) 1 mRNA, which is involved in reverse cholesterol transfer. The aim of this study was to evaluate the effect of aerobic exercise on ABCA1 mRNA expression in PBMC cells.
Materials and Methods: This study was performed on 30 post-menopausal women who were randomly divided into two groups of control and eight weeks of aerobic exercise.
Then, before and after the intervention, blood samples were taken from all subjects in 10 cc and lymphocytes were isolated by centrifugation and mRNA was isolated and assessed by PCR and the information obtained were analyzed by covariance tests with significance at P <0.05 in SPSS19.
Results: The results showed that aerobic exercise had a significant effect on the expression of ABCA1, HDL, LDL and APO-A1 genes (P <0.05).
Conclusion: Based on the research findings, it can be concluded that eight weeks of aerobic exercise improves reverse cholesterol transfer and prevents atherosclerotic factors in post-menopausal women.
Keywords
Aerobic exercise
Peripheral blood mononuclear cell
ATP-binding cassette transporter
Reverse cholesterol transport
Menopausal
Subjects
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Daneshvar Medicine
Volume 32, Issue 4 - Serial Number 173
November and December 2024
Pages 68-76