Investigating repeated exposure to sevoflurane during adolescence and their effects on behavioral and pathological impairment in adult male and female Wistar rats

Document Type : Original Article

Authors

1 Electrophysiology Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran

2 Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.

3 Imam Khomeini Hospital Complex, Department of Anesthesiology, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Background and Objective: Repeated anesthesia in adolescence is necessary for some therapeutic interventions, such as radiotherapy or burns. Research has indicated that exposure to general anesthetics during the adolescent period can induce cell death, cognitive and behavioral problems, and later neurobehavioral problems in adulthood. The current study was aimed at providing a detailed morphological and functional evaluation of the long-term impacts of repeated exposure to sevoflurane.
Materials and Methods: In this study, 28 adolescent rats were randomly divided into male and female control groups and inhaled sevoflurane (at a concentration of 2%) daily for 15 days. After last anesthesia, animals of all groups were cared for 30-35 days, and then the effects of repeated exposure to sevoflurane on cognitive functions and anxiety behavior were tested by the Morris water maze, open field, and elevated plus maze. Effects of sevoflurane on cell death and the increase of dark neurons were compared in CA1 of the hippocampus and brain cortex. Data were evaluated using one-way and two-way ANOVA.
Results: Our results indicated that repeated exposures to sevoflurane during adolescence can cause behavioral problems later in adulthood. We found a significant increase in dark neurons and cell death in the sevoflurane group compared to the control group in both male and female animals.
Conclusion: These results may provide a new perspective for understanding the mechanism of repeated exposure to sevoflurane-induced toxic effects in adolescence.

Keywords

Main Subjects

References

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Daneshvar Medicine
Volume 31, Issue 6 - Serial Number 169
March and April 2024
Pages 60-74

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