Daneshvar Medicine

Daneshvar Medicine

Identification, isolation and evaluation of the antibiotic resistance pattern of Stenotrophomonas maltophilia isolated from blood samples of patients referred to medical centers in Qazvin in 2022

Document Type : Original Article

Authors
Fatemeh Sameni 1
Maedeh Pourali Eshkalak 2
Bahareh Hajikhani 3
Mona Ghazi 3
Masoud Dadashi 4
1 Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran
2 Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran
3 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
10.22070/daneshmed.2023.17810.1361
Abstract
Background and Objective: Stenotrophomonas maltophilia (S. maltophilia) is a non-fermenting gram-negative bacterium that has rapidly emerged as an important hospital pathogen in hospitalized patients and is considered a global threat due to the increase of intrinsic antibiotic resistance. The present study was conducted to determine the antibiotic resistance profile of S. maltophilia isolates collected from blood cultures of patients referred to Qazvin hospitals.
Materials and Methods: In this study, 28 blood samples with the early diagnosis of S. maltophilia were collected. First, the identification of bacteria was performed by using culture and common biochemical tests. The final confirmation of the samples was done by examining 23S rRNA gene using the molecular PCR method. Finally, antibiogram was performed using disc diffusion method according to CLSI 2022 guidelines.
Results: 60.71% of patients with positive blood culture were male. The resistance rates to levofloxacin, minocycline and trimethoprim-sulfamethoxazole were 10.71%, 7.14% and 3.57%, respectively.
Conclusion: Considering the appropriate effectiveness of the trimethoprim-sulfamethoxazole, it can be concluded that the isolates are still sensitive to this antibiotic and it is still considered as the best therapeutic option. It is necessary to evaluate the levels of antibiotic resistance using standard methods before prescribing the antibiotics in order to prevent the emergence and spread of resistant strains in the community and the possibility of treatment failure.
Keywords
Stenotrophomonas maltophilia
Antibiotic resistance
Disc diffusion
Subjects
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Daneshvar Medicine
Volume 31, Issue 4 - Serial Number 167
November and December 2023
Pages 20-27