Daneshvar Medicine

Daneshvar Medicine

The effect of 8 weeks of endurance-resistance training with taurine supplementation on the serum level of osteopontin and osteocalcin in Wistar diabetic male rats treated with streptozocin

Document Type : Original Article

Authors
Fatemeh Akbari Vargesaran
Ameneh Pourrahim Ghouroghchi
Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
10.22070/daneshmed.2023.16988.1292
Abstract
Background and Objective: There is a relationship between osteopontin and osteocalcin bone indices and diabetes and osteoporosis. The aim of this study was to determine the effect of 8 weeks of endurance training with taurine supplementation on the serum level of osteopontin and osteocalcin in male Wistar rats with streptozocin.
Materials and Methods: In this experimental study, 30 adult non-nursing rats with a weight range of 250-300 grams and 6 weeks old were randomly divided into 3 groups including two diabetic groups, diabetic exercise+supplement (n=10) and diabetic control (n=10) and a non-diabetic group, healthy control (n=10). To induce diabetes, at the end of the eighth week, the amount of 55 mg per kilogram of body weight of streptozocin (STZ) was injected intraperitoneally. The exercise+diabetic supplement group performed combined exercises for eight weeks with a frequency of 5 times a week (endurance exercise with an intensity of 75% Vo2max on the treadmill and then strength training, climbing the ladder 15 times). At the same time as the exercises started, taurine supplement was added to the rats' food. One-way ANOVA and post hoc Bonferroni test were used to compare the mean of the variables between-group and paired samples T test was used to compare the mean of the variables intra-group.
Results: Osteopontin decreased significantly in the diabetic exercise+supplement group compared to the diabetic control group (P<0.001); On the other hand, there was a significant increase of 12.118% in the diabetic control group compared to the healthy control group (P<0.001). While, osteocalcin increased significantly in the exercise+diabetic supplement group compared to the diabetic control group (P<0.001) and decreased significantly in the diabetic control group compared to the healthy control group (p<0.001).
Conclusion: Eight weeks of endurance training with taurine supplementation led to a decrease in osteopontin and an increase in osteocalcin. Therefore, people with diabetes and prone to osteoporosis are recommended to do combined exercises to prevent the development of this diseases.
Keywords
Endurance-resistance training
Osteopontin
Osteocalcin
Diabetes
Taurine supplement
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Daneshvar Medicine
Volume 31, Issue 1 - Serial Number 164
May and June 2023
Pages 34-45