Daneshvar Medicine

Daneshvar Medicine

The effect of aqueous and ethanolic extracts of chamomile on the vital activity of peripheral blood leukocytes of patients with covid-19

Document Type : Original Article

Authors
Rasoul Rashidi 1
Tooba Ghazanfari 2
Tayabeh Radjabian 2
Ensie Sadat Mirsharif 2
1 Department of immunology, Faculty of Medicine, Shahed University, Tehran, Iran
2 Immunoregulation Research Center, Shahed University, Tehran, Iran
10.22070/daneshmed.2023.17141.1304
Abstract
Background and Objective: Numerous studies have shown the anti-inflammatory, antimicrobial, anti-allergic, anti-cancer, anti-spasm, analgesic and wound healing effects of chamomile (Matricaria chamomile). In this study, the effect of aqueous and ethanolic extracts of chamomile on the vital activity of cultured leukocytes of patients with covid-19 was investigated.
Materials and Methods: After preparation of aqueous and ethanolic extracts of chamomile, peripheral blood mononuclear cells (PBMC) of patients with covid-19 with doses of 200, 300, 400 and 500 micrograms/ml of ethanolic extract of chamomile and doses of 400, 500, 600 and 700 µg/ml chamomile aqueous extract were treated. After 24 hours, the vital activity of PBMCs was measured by MTT method. To analyze the findings, one-way analysis of variance was used in SPSS software version 24. P<0.05 was considered significant.
Results: In the current research, chamomile ethanol extract in doses of 500 micrograms/ml and aqueous extract in doses of 600 and 700 micrograms/ml led to a significant decrease in the vital activity of peripheral blood mononuclear cells compared to the control group.
Conclusion: Aqueous and ethanol extracts of chamomile significantly decrease the vital activity of peripheral blood mononuclear cells; This decrease in vital activity is dose-dependent, and with increasing dose, a decrease in vital activity is observed.
Keywords
Chamomile
Covid-19
Peripheral blood mononuclear cells
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Daneshvar Medicine
Volume 30, Issue 6 - Serial Number 163
January and February 2023
Pages 1-9