Daneshvar Medicine

Daneshvar Medicine

Study on the association between interleukin-6 receptor Asp358Ala (rs2228145) single nucleotide polymorphism, with the severity of clinical symptoms in patients with COVID-19

Document Type : Original Article

Authors
Azadeh Rashidi 1
Sara Ghaffarpour 2
Mohammad Saber Zamani 3
Mohammad Mahdi Naghizadeh 4
Tooba Ghazanfari 2
1 Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran
2 Immunoregulation Research Center, Shahed University, Tehran, Iran
3 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Non-communicable Diseases Research Center, Fasa University of Medical Science, Fasa, Iran
10.22070/daneshmed.2022.15135.1121
Abstract
Background and Objective: Increasing of interleukin-6 (IL-6) in Covid-19 patients has been reported in various studies. Genetic variants of the IL-6 receptor are involved in the beginning and progression of severe infections, autoimmunity, and also inflammatory diseases. The aim of this study was to investigate the association between SNP (rs2228145) of IL-6 receptor with the severity of Covid-19 patients.
Materials and Methods: In the present cross-sectional and analytical study, 180 patients with Covid-19 entered voluntarily and were classified based on the severity of the disease in 4 groups: mild (90 patients, age 46.40 ± 15.8), moderate (45 patients, age 50.4 ±16.4), severe (25 patients, age 59.70 ±24) and critical (20 patients, age 15.30 ± 60.8). Peripheral blood samples were taken from patients and after DNA extraction, genotyping was performed by RFLP PCR and electrophoresis on 2% agarose gel. Data were analyzed using SPSS software and Chi-Square or ANOVA.
Results: The mean age of patients in the outpatient group and the intubated inpatients were 46.40 ± 15.8 and 60.30 ± 15.8, respectively (P-value <0.001). Also, the frequency of diabetic patients in the outpatient group and intubated group were about 21% and 45%, respectively (P-value <0.05). A significant difference was observed in the CC genotype between outpatient and inpatient groups (P-value <0.01) as well as intubated patients (P-value <0.05).
Conclusion: The frequency of rs2228145 variants in Covid-19 patients with different severity is diverse and CC genotype can be a predictor of prognosis in admission to the ICU and intubation.
Keywords
Covid-19
Polymorphism
Interleukin-6 receptor
rs2228145
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Daneshvar Medicine
Volume 30, Issue 1 - Serial Number 158
May and June 2022
Pages 1-11