The effects of aerial parts hydroalcoholic extract of Lavandula dentata in the pilocarpine rat model of temporal lobe epilepsy

Document Type : Original Article

Authors

1 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran

2 Neurophysiology Research Center, Shahed University, Tehran, Iran

Abstract

Background and Objective: Lavandula dentata is called Ustukhuddus in Iran .There are some reports about the anticonvulsant effects of Ustukhuddus but, the effect of this plant on epilepsy is not reported yet. In this study the effect of Lavandula dentata on seizures induced by pilocarpine was investigated.
Materials and Methods: A total of 75 male rats weighing 200-250 grams were divided into five groups of 15, which included: 1- control saline 2- control extract 3- Epileptic group 4- Epileptic group treated with single doses of lavender extract 200 mg / Kg  5- epileptic group treated with repeated dose of lavender extract (200 mg / Kg). The induction of seizures in all three groups was done by intra-peritoneal injection of pilocarpine hydrochloride (380 mg / kg).  Brain malondialdehyde (MDA) and glutathione was measured. Statistical analysis was carried out using one way ANOVA or Kruskal–Wallis oneway analysis of variance by ranks followed by post-hoc tukey test.
Results: The administration of Lavandulla dentata in the pretreatment groups delayed the onset of status epilepticus (SE), reduced the duration of SE and decreased the mortality rate compared to the epileptic group. Pretreated extract increased significantly brain glutathione levels 24 hours after pilocarpine administration, but did not exhibit inhibitory effect on brain MDA.
Conclusion: Lavendula dentata reduced significantly seizure intensity and the mortality rate due to SE induced by pilocarpine administration. It seems that antioxidant activity such as brain glutathione enhancement was partly responsible for such results.  

Keywords

References

  1. Thurman DJ, Beghi E, Begley CE, Berg AT, Buchhalter JR, Ding D, et al. Standards for epidemiologic studies and surveillance of epilepsy. Epilepsia 2011; 52 Suppl 7: 2–26.
  2. Soltanzade A. Brain and nerves and muscles. Nasher, Heydari 1383; 236.
  3. Vezzani M, French J, Bartfai T, Baram TZ. The role of inflammation in epilepsy. Nature Reviews Neurology 2011; 7: 31-40.
  4. Theodore WH, Fisher R. Brain stimulation for epilepsy. Acta Neurochirurgica Supplement 2007; 97(2): 261-272
  5. Banerjee PN, Filippi D, Allen Hauser W. The descriptive epidemiology of epilepsy-a review. Epilepsy Research 2009; 85(1):31-45.
  6. Riviello JJ. Classification of seizures and epilepsy. Current Neurology and Neuroscience Reports 2003; 3(4):325-31.
  7. McHugh JC, Delanty N. Epidemiology and classification of epilepsy: gender comparisons. International Review of Neurobiology 2008; 83:11-26.
  8. Jefferys JG. Advances in understanding basic mechanisms of epilepsy and seizures. Seizure 2010; 19(10):638-46.
  9. Dichter MA. Emerging concepts in the pathogenesis of epilepsy and epileptogenesis. Archives of Neurology 2009; 66(4):443-7.
  10. Leroy C, Roch C, Koning E, Namer IJ, Nehlig A. In the Lithium-Pilocarpine Model of Epilepsy, Brain Lesions are not Linked to Changes in Blood-Brain Barrier Permeability: An auto radiographic study in adult and developing rats. Experimental Neurology 2003; 182: 361-372.
  11. Winawer MR, Makarenko N, McCloskey DP, Hintz TM, Nair N, Palmer AA, Scharfman HE. Acute and chronic responses to the convulsant pilocarpine in DBA/2J and A/J mice. Neuroscience 2007; 149(2): 465-475.
  12. van Vliet EA, Aronica E, Gorter JA. Role of blood-brain barrier in temporal lobe epilepsy and pharmacoresistance. Neuroscience 2014; 277: 455-73.
  13. Muazu J, Kaita A H. A review of traditional plants used in the treatment of epilepsy amongst the hausa/Fulani tribes of northern Nigeria. African Journal of Traditional, Complementary, and Alternative Medicines. 2008; 5(4): 387-90.
  14. Carvey PM. Drug action in the CNS. New_York: Oxford University Press 1998; 201.
  15. Gharegozli K, Khoshraftar A, Amini Z. Epilepsy: Treatment and anesthesia methods. Hamedan University of Medical Sciences 1386: 9.
  16. Zarghami M, Shaikholeslami M. Psychiatric aspects of epilepsy. First edition, Sari, Mazandaran University of Medical Sciences and Health Services 1378:152.
  17. Pakdaman H, Shahbazi A. Epilepsy. First edition, Tehran, Esharat Publishing Center 1374: 28.
  18. Adams RD, Victor M, Allan R. Principles of neurology. Mc-Graw Hill 1997; 25: 313-330.
  19. McLachlan RS. Vagus Nerve Stimulatiom for Intractable epilepsy. Clinical Neurophysiology 1997; 14(5): 358-68.
  20. Katzung B, Mesters S, Terose A. Katzung Basic and Clinical Pharmacology. Translated by: Fatollahi A, Sobhanian KH, Hassanpoor N, Hagh Nazarian A. Second edition, Tehran, Arjomand book 1393: 555-590.
  21. Beheshtipoor N, Jamali Moghaddam N, Splaimani S, Haghnegahdar A, Salehi A. Evaluation of patients' knowledge, belief and practice about herbal medicines in clients of one of the clinics of Shiraz University of Medical Science. Journal of Herbal Medicine 1389;1 (4):53-56.
  22. Sharafkandi AB. Translation of Canon in Medicine Avesina.1991 fifth ed. Tehran: Soroosh pub: 66.
  23. Heydari A, Rahmati B, Khalili M, Roghani M, Zaeri F. Intensified convulsions induced through intravenous infusion of PTZ by Nepeta menthoides hydroalcoholic extract in mice. Daneshvar Medicine 2015; 22(119): 23.
  24. Rahmati B, Khalili M, Roghani M, Ahghari P. Anti- convulsant effect of hydroalcoholic extract of Lavandula officinalis on seizures in pentylenetetrazol -induced kind-ling model in male mice. Daneshvar Medicine 2012; 19(98):1-8.
  25. Gilani AH, Aziz N, Khan MA. Ethnopharmacological evaluation of the anticonvulsant, sedative and antispasmodic activities of Lavandula stoechas L. Journal of Ethnopharmacology 2000; 71(1): 161-7.
  26. Gloor P, Neurobiological substrates of ictal behavioral changes. Advances in neurology 1991; 55:1-34.
  27. Veliskova J, behavioral characterization of seizures in rats. In: Pitkanen A, Schwartskroin PA, Solomon LM, editors. Models of Seizures and Epilepsy. Burlington: Elsevier Academic Press 2006: 601-611.
  28. Mehrabani M, Modirian E, Ebrahimabadi AR, Shahna-vaz S, Vafazadeh S, Heidari MR. The study of effects of Hydro methanolic extracts of lavandula vera DC and Cuscuta Epithymum Murr on the seizure induced by pentylene tetrazole in mice. Journal of Kerman University of Medical Sciences 2007; 14(1):25-32.
  29. Shahriyari H, Ersali A, Rahmanifard M. Anticonvulsant effect of Lavandula officinalis in two epilepsy animal model. Journal of Iran Medical Basic Sciences 2004; 8(3):172-178.
  30. R.M Freitas, F.C.F Sousa, S.M.M Vasconcelos, G.S.B Viana, M.M.F Fonteles. Pilocarpine-induced status epilepticus in rats: lipid peroxidation level, nitrite formation, GABAergic and glutamatergic receptor alterations in the hippocampus, striatum and frontal cortex. Pharmacology Biochemistry and Behavior 2004; 78(2): 327-332.
  31. Shakeel S, Rehman MU, Tabassum N, Amin U, Mir MUR. Effect of Naringenin (A naturally occurring flavanone) Against Pilocarpine-induced Status Epilepticus and Oxidative Stress in Mice. Pharmacognosy Magazine 2017; 13(1): 154–160.
  32. Tome AR, Feng D, Freitas RM. The effects of alpha-tocopherol on hippocampal oxi-dative stress prior to in pilocarpine-induced seizures . Neurochemical Research 2010; 35(4): 580-587.
  33. Draper HH, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. Methods in Enzymology 1990; 186: 1-85.
  34. Sajadi E, Naderi GA, Ziaee R. Antioxidant Effects of Some Medicinal Plants. Journal of Kermanshah University of Medical Sciences 2004; 2(8).
  35. Sebai H, Selmi S, Rtibi K, Gharbi N, Sakly M.  Protective effect of Lavandula stoechas and Rosmarinus Officinalis essential oils against reproductive damage and oxidative stress in alloxan-induced diabetic rats. Journal of Medicinal Food 2015 Feb; 18(2): 241-9.
  36. Yuksel A, Cengiz M, Seven M, Ulutin T. Erythrocyte  glutathione, glutathione pero-xidase, superoxide dismutase and serum lipid peroxidation in epileptic children with valproate and carbamazepine monotherapy. Journal of Basic and Clinical Physiology and Pharmacology 2000; 11(1):73–81.
  37. Yuksel A, Cengiz M, Seven M, Ulutin T. Changes in the antioxidant system in epil-eptic children receiving antiepileptic drugs: two-year prospective studies. Journal of ChildNeurology 2001; 16(8): 603–606.
  38. Sołowiej E, Sobaniec W. The effect of antiepileptic drug therapy on antioxidant enzyme activity and serum lipid peroxidation in young patients with epilepsy. Neurologia i Neurochirurgia Polska 2003; 37(5): 991–1003.
  39. Martınez-Ballesteros C, Pita-Calandre E, Sanchez-Gonz alez Y, Rodrıguez-Lopez C.M, Agil A. Lipid peroxidation in adult epileptic patients treated with valproic acid. Revista de Neurologia 2004; 38(2):101–106.
  40. Nourooz zadeh J, Eftekhar A. Physiological importance of glutathione in health and disease. Birjand Scientific Journal of University Medical Sciences 1386; 3 (14): 5-12.
  41. Algieri F, Rodriguez-Nogales A, Vezza T, Garrido-Mesa J, Garrido-Mesa N, Utrilla MP, et al. Anti-inflammatory activity of hydroalcoholic extracts of Lavandula dentata L. and Lavandula stoechas L .  Journal of Ethnopharmaco-logy 2016; 22(190): 58-142.
Daneshvar Medicine
Volume 29, Issue 1 - Serial Number 152
March and April 2021
Pages 35-48

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