The effect of pumpkin seed and endurance training on oxidative stress factors and DNA damage

Document Type : Original Article

Authors

Abstract

Objective: This study aimed to investigate the effect of pumpkin seed and endurance training on oxidative stress factors and DNA damage of slow-twitch muscle tissue in male rats poisoned with hydrogen peroxide.
Materials and Methods: A total of 48 adult male rats weighing 220±20 g at eight weeks were administered in accordance with stress interventions (hydrogen peroxide), pumpkin seed and endurance training. To induce oxidative stress, intraperitoneal injection of H2O2 at a dose of 2 mmol/kg was performed three times a week. Inoculation of pumpkin seeds was performed at 0.5 μg per day with intraperitoneal injection at a concentration of 300000 U/ml. The training groups performed daily aerobic exercise on a treadmill for 8 weeks, the endurance training protocol started at a speed of 8 m/min and a slope of 10 degrees for 30 minutes on the treadmill in the first week and gradually at a speed of 20 m/min at an angle of ten. The degree reached 60 minutes in the eighth week. Data were analyzed using repeated- measures analysis of variance and Bonferroni post hoc test at the level of α = 0.05.
Results: The results of the present study showed that training and receiving pumpkin seeds separately has a significant effect on ATP, ADP, ATP / ADP ratio, MDA and PAB of horseshoe muscle (P= 0.001); But the interaction between training and pumpkin seeds did not have a significant effect only on MDA of soleus muscle (P = 0.025) but had a significant effect on other variables (P = 0.001).
Conclusion: Taking pumpkin seed supplement along with endurance training can reduce oxidative stress in the body.

Highlights

  1. Wang M, Zheng Y. Oxidative stress and antioxidants in the trabecular meshwork. Peer Journal 2019;7:e8121.
  2. Mohammadjafari H, Arazi H, Nemati N, Bagherpoor T, Suzuki K. Acute Effects of Resistance Exercise and the Use of GH or IGF-1 Hormones on Oxidative Stress and Antioxidant Markers in Bodybuilders. Antioxidants 2019;8(12):587.
  3. Madany J. Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract. Polish Journal of Veterinary Sciences 2016;19(2):429-31.
  4. Fairus AM, Choudhary B, Hosahalli S, Kavitha N, Shatrah O. Dihydroorotate dehydrogenase (DHODH) inhibitors affect ATP depletion, endogenous ROS and mediate S-phase arrest in breast cancer cells. Biochimie 2017;135:154-63.
  5. Francesca M, Tania F, Riccardo M, Michele M, Tania G, Pietro M, Alessandra M. Oxidative stress in exercise training: the involvement of inflammation and peripheral signals. Free Radical Research 2019;1-301.
  6. Kattoor AJ, Pothineni NV, Palagiri D, Mehta JL. Oxidative stress in atherosclerosis. Current Atherosclerosis Reports 2017;19(11):42.
  7. Liang R, Shao X, Shi Y, Jiang L, Han G. Antioxidant defenses and metabolic responses of blue mussels (Mytilus edulis) exposed to various concentrations of erythromycin. Science of The Total Environment 2020;698:134221.
  8. Park SY, Kwak YS. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. Journal of Exercise Rehabilitation 2016;12(2):113.
  9. Tartibian B, Baghaei B, Baradaran B. Catalase enzyme gene expression and oxidant markers’ levels in trained women: effect of incremental exercise. Journal of Shahid Sadoughi University of Medical Sciences 2013;20(6):778-88.
  10. Shiri E, Rahnema M, Bigdeli M. The effect of pumpkin seed oil (Cucurbita Moschata) on the permeability of the blood -brain barrier and on brain edema in stroke animal model. Journal of North Khorasan University of Medical Sciences 8(2):301-311.
  11. Ghanbari-Niaki A, Fathi R, and Hedayati M. Effect of 8 Weeks Endurance Training With Two Different Durations on Plasma HDL-Ghrelin in Male Rats. Iranian Journal of Endocrinology and Metabolism 2011;13(3):309-314.
  12. Seif HS. Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni-Suef University Journal of Basic and Applied Sciences 2014;3(3):178-85.
  13. Chen L, Huang G. Extraction, characterization and antioxidant activities of pumpkin polysaccharide. International Journal of Biological Macromolecules 2018;118:770-4.
  14. Zhao S, Liang T, Zhou T, Li D, Wang B, et al. Biotransformation and responses of antioxidant enzymes in hydroponically cultured soybean and pumpkin exposed to perfluorooctane sulfonamide (FOSA). Ecotoxicology and Environmental Safety 2018;161:669-75.
  15. Fusco D, Colloca G, Monaco M R L, Cesari M. Effects of antioxidant supplementation on the aging process. Clinical Interventions in Aging 2007; 2(3):377.
  16. Dabidi Roshan V, Moslehi Najafabadi E. The Effect of short-term vitamin E supplementation on some indexes of sport performances and lipid per-oxidation in healthy men. World Journal of Sport 2009; 2(2):75-81.
  17. Gomes E C, Silva A N, Oliveira M R. Oxidants, antioxidants, and the beneficial roles of exercise-induced production of reactive species. Oxidative Medicine and Cellular Longevity 2012:756132.
  18. Holeček M, Vodeničarovová M. Effects of histidine load on ammonia, amino acid, and adenine nucleotide concentrations in rats. Amino Acids 2019; 11:1-4.
  19. Ghanbari-Niaki A, Kraemer R, Abednazari H. Time-course alterations of plasma and soleus agouti-related peptide and relationship to ATP, glycogen, cortisol, and insulin concentrations following treadmill training programs in male rats. Hormone and Metabolic Research 2011; 43(2): 112-116.
  20. Houghton C R, Hawkins R A, Williamson D H, Krebs H A. The effects of physical training on the metabolic response to short-term severe exercise in the rat. Biochemical Journal 1971;124(5): 57P.
  21. Broxterman RM, Layec G, Hureau TJ, Morgan DE, Bledsoe AD, et al. Bioenergetics and ATP synthesis during exercise: role of group III/IV muscle afferents. Medicine and Science in Sports and Exercise 2017;49(12):2404.
  22. Gastin PB. Energy system interaction and relative contribution during maximal exercise. Sports Medicine 2001;31(10):725-41.
  23. Zieliński J, Slominska EM, Król-Zielińska M, Krasiński Z, Kusy K. Purine metabolism in sprint-vs endurance-trained athletes aged 20‒90 years. Scientific Reports 2019;9(1):1-0.
  24. Puthucheary ZA, Astin R, Mcphail MJ, Saeed S, Pasha Y, Bear DE, Constantin D, Velloso C, Manning S, Calvert L, Singer M. Metabolic phenotype of skeletal muscle in early critical illness. Thorax 2018;73(10):926-35.
  25. Habibian M, Dabidi Roshan V, Moosavi SJ, Mahmoody SA. Neuroprotective effect of aerobic training against Lead-induced oxidative stress in rat cerebellum. Journal of Gorgan University of Medical Sciences 2013;15(3):39-45.
  26. Azamian Jazi A, Shokouhi R. The Effect of an Eight Week Combined Exercise Training on Oxidative Stress and Lipid Peroxidation in Postmenopausal Women with Type 2 Diabetes. Journal of Shahid Sadoughi University of Medical Sciences 2016;24(8):667-78.
  27. Marosi K, Bori Z, Hart N, Sárga L, Koltai E, Radák Z, Nyakas C. Long-term exercise treatment reduces oxidative stress in the hippocampus of aging rats. Neuroscience 2012;226:21-8.
  28. Bellafiore M, Bianco A, Battaglia G, Naccari MS, Caramazza G, et al. Training session intensity affects plasma redox status in amateur rhythmic gymnasts. Journal of Sport and Health Science 2019;8(6): 561–566.

Keywords

References

  1. Wang M, Zheng Y. Oxidative stress and antioxidants in the trabecular meshwork. Peer Journal 2019;7:e8121.
  2. Mohammadjafari H, Arazi H, Nemati N, Bagherpoor T, Suzuki K. Acute Effects of Resistance Exercise and the Use of GH or IGF-1 Hormones on Oxidative Stress and Antioxidant Markers in Bodybuilders. Antioxidants 2019;8(12):587.
  3. Madany J. Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract. Polish Journal of Veterinary Sciences 2016;19(2):429-31.
  4. Fairus AM, Choudhary B, Hosahalli S, Kavitha N, Shatrah O. Dihydroorotate dehydrogenase (DHODH) inhibitors affect ATP depletion, endogenous ROS and mediate S-phase arrest in breast cancer cells. Biochimie 2017;135:154-63.
  5. Francesca M, Tania F, Riccardo M, Michele M, Tania G, Pietro M, Alessandra M. Oxidative stress in exercise training: the involvement of inflammation and peripheral signals. Free Radical Research 2019;1-301.
  6. Kattoor AJ, Pothineni NV, Palagiri D, Mehta JL. Oxidative stress in atherosclerosis. Current Atherosclerosis Reports 2017;19(11):42.
  7. Liang R, Shao X, Shi Y, Jiang L, Han G. Antioxidant defenses and metabolic responses of blue mussels (Mytilus edulis) exposed to various concentrations of erythromycin. Science of The Total Environment 2020;698:134221.
  8. Park SY, Kwak YS. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. Journal of Exercise Rehabilitation 2016;12(2):113.
  9. Tartibian B, Baghaei B, Baradaran B. Catalase enzyme gene expression and oxidant markers’ levels in trained women: effect of incremental exercise. Journal of Shahid Sadoughi University of Medical Sciences 2013;20(6):778-88.
  10. Shiri E, Rahnema M, Bigdeli M. The effect of pumpkin seed oil (Cucurbita Moschata) on the permeability of the blood -brain barrier and on brain edema in stroke animal model. Journal of North Khorasan University of Medical Sciences 8(2):301-311.
  11. Ghanbari-Niaki A, Fathi R, and Hedayati M. Effect of 8 Weeks Endurance Training With Two Different Durations on Plasma HDL-Ghrelin in Male Rats. Iranian Journal of Endocrinology and Metabolism 2011;13(3):309-314.
  12. Seif HS. Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni-Suef University Journal of Basic and Applied Sciences 2014;3(3):178-85.
  13. Chen L, Huang G. Extraction, characterization and antioxidant activities of pumpkin polysaccharide. International Journal of Biological Macromolecules 2018;118:770-4.
  14. Zhao S, Liang T, Zhou T, Li D, Wang B, et al. Biotransformation and responses of antioxidant enzymes in hydroponically cultured soybean and pumpkin exposed to perfluorooctane sulfonamide (FOSA). Ecotoxicology and Environmental Safety 2018;161:669-75.
  15. Fusco D, Colloca G, Monaco M R L, Cesari M. Effects of antioxidant supplementation on the aging process. Clinical Interventions in Aging 2007; 2(3):377.
  16. Dabidi Roshan V, Moslehi Najafabadi E. The Effect of short-term vitamin E supplementation on some indexes of sport performances and lipid per-oxidation in healthy men. World Journal of Sport 2009; 2(2):75-81.
  17. Gomes E C, Silva A N, Oliveira M R. Oxidants, antioxidants, and the beneficial roles of exercise-induced production of reactive species. Oxidative Medicine and Cellular Longevity 2012:756132.
  18. Holeček M, Vodeničarovová M. Effects of histidine load on ammonia, amino acid, and adenine nucleotide concentrations in rats. Amino Acids 2019; 11:1-4.
  19. Ghanbari-Niaki A, Kraemer R, Abednazari H. Time-course alterations of plasma and soleus agouti-related peptide and relationship to ATP, glycogen, cortisol, and insulin concentrations following treadmill training programs in male rats. Hormone and Metabolic Research 2011; 43(2): 112-116.
  20. Houghton C R, Hawkins R A, Williamson D H, Krebs H A. The effects of physical training on the metabolic response to short-term severe exercise in the rat. Biochemical Journal 1971;124(5): 57P.
  21. Broxterman RM, Layec G, Hureau TJ, Morgan DE, Bledsoe AD, et al. Bioenergetics and ATP synthesis during exercise: role of group III/IV muscle afferents. Medicine and Science in Sports and Exercise 2017;49(12):2404.
  22. Gastin PB. Energy system interaction and relative contribution during maximal exercise. Sports Medicine 2001;31(10):725-41.
  23. Zieliński J, Slominska EM, Król-Zielińska M, Krasiński Z, Kusy K. Purine metabolism in sprint-vs endurance-trained athletes aged 20‒90 years. Scientific Reports 2019;9(1):1-0.
  24. Puthucheary ZA, Astin R, Mcphail MJ, Saeed S, Pasha Y, Bear DE, Constantin D, Velloso C, Manning S, Calvert L, Singer M. Metabolic phenotype of skeletal muscle in early critical illness. Thorax 2018;73(10):926-35.
  25. Habibian M, Dabidi Roshan V, Moosavi SJ, Mahmoody SA. Neuroprotective effect of aerobic training against Lead-induced oxidative stress in rat cerebellum. Journal of Gorgan University of Medical Sciences 2013;15(3):39-45.
  26. Azamian Jazi A, Shokouhi R. The Effect of an Eight Week Combined Exercise Training on Oxidative Stress and Lipid Peroxidation in Postmenopausal Women with Type 2 Diabetes. Journal of Shahid Sadoughi University of Medical Sciences 2016;24(8):667-78.
  27. Marosi K, Bori Z, Hart N, Sárga L, Koltai E, Radák Z, Nyakas C. Long-term exercise treatment reduces oxidative stress in the hippocampus of aging rats. Neuroscience 2012;226:21-8.
  28. Bellafiore M, Bianco A, Battaglia G, Naccari MS, Caramazza G, et al. Training session intensity affects plasma redox status in amateur rhythmic gymnasts. Journal of Sport and Health Science 2019;8(6): 561–566.
Daneshvar Medicine
Volume 28, Issue 3 - Serial Number 148
July and August 2020
Pages 28-41

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