The Effect of High-Intensity Interval Training and Nanoselenium Supplementation on Pancreatic Glucose Transporter 4 and Myonectin Gene Expression in Dexamethasone-Induced Rats

چگینی, Chegini; Hosseini, Masoumeh; Riahi, Shahin; Dehghani, Mahmoud

doi:10.22070/daneshmed.2026.20627.1616

The Effect of High-Intensity Interval Training and Nanoselenium Supplementation on Pancreatic Glucose Transporter 4 and Myonectin Gene Expression in Dexamethasone-Induced Rats

Document Type : Original Article

Authors

chegini چگینی ¹

masoumeh hosseini ¹

Shahin Riahi ¹

Mahmoud Dehghani ²

¹ Department of Physical Education and Sport Sciences, ET.C. , Islamic Azad University, Tehran, Iran.

² Department of Humanities and Sport Sciences, Najafabad, Islamic Azad University, Isfahan, Iran.

10.22070/daneshmed.2026.20627.1616

Abstract

Objective: This study aimed to investigate the effect of nano-selenium supplementation and high-intensity interval training (HIIT) on the expression of Glut4 and myonectin genes in rats with pancreatitis induced by dexamethasone.
Methods: In this experimental study, 40 male Wistar rats aged 8 weeks were randomly divided into five groups: healthy control, induced control, induced + exercise, induced + supplement, and induced + exercise + supplement. To induce pancreatitis, dexamethasone was injected intraperitoneally at a dose of 0.4 mg/kg for three days. HIIT included running on a treadmill at 75–100% VO₂max, six days per week for four weeks. Nano-selenium (100 mg/kg with a particle size of 250 nm) was administered via gavage every other day for the same duration. Gene expression levels were measured using RT-PCR after tissue sampling. Data were analyzed using two-way ANOVA followed by Tukey's post-hoc test.
Results: Significant increases in Glut4 gene expression were observed in the exercise, supplement, and combined groups compared to the induced control group (P=0.0001). Similarly, significant upregulation of myonectin was seen in the exercise and combined groups (P=0.0001), but not in the supplement-only group (P=0.999).
Conclusion: Both HIIT and nano-selenium supplementation positively influenced Glut4 and myonectin gene expression. However, the combination of HIIT and nano-selenium showed the most pronounced effects compared to individual interventions.

Keywords

High-intensity interval training

nanoselenium supplementation

glucose transporter 4

myonectin

dexamethasone

Subjects