Daneshvar Medicine

Daneshvar Medicine

The effect of high-intensity interval training (HIIT) on the content of eIF2α, ATF4 and CHOP proteins in the left ventricle of obese diabetic rats

Document Type : Original Article

Authors
Hamed Alizadeh Pahlavani 1
Mohammad Sherafati Moghadam 2
Fatemeh Solhdoust 2
1 Department of Physical Education, Farhangian University, Tehran, Iran
2 Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran
10.22070/daneshmed.2025.19909.1573
Abstract
Background and Objective: The prevalence of obesity and diabetes is an epidemic and poses risks to public health. Therefore, the aim of the present study was to determine the effect of HIIT on the content of eIF2α, ATF4 and CHOP proteins in the left ventricle of obese diabetic rats.
Materials and Methods: The present study was experimental-basic. Twelve 2-month-old male Sprague-Dawley rats weighing 180±20 g were purchased. To induce type 2 diabetes, nicotinamide and streptozotocin solutions were injected at doses of 110 and 60 mg/kg, respectively. Blood sugar between 126 and 260 mg/dl was considered an index of type 2 diabetes. Rats were divided into control and HIIT groups. The HIIT group trained for 8 weeks, 3 days a week, with 5 4-minute sessions at an intensity of 85-95% of maximum speed, followed by 3-minute active rest periods at an intensity of 50-60% of maximum speed. After 48 hours from the last training session, the rats were anesthetized and left ventricular tissue was removed and the desired proteins were measured by Western blotting. The Shapiro-Wilk test and independent t-test were used to examine the data, and a significance level of P < 0.05 was considered.
Results: After 8 weeks of HIIT, the content of eIF2α (P=0.001), ATF4 (P=0.001), and CHOP (P=0.001) proteins in the left ventricle of the heart showed a significant increase compared to the control group.
Conclusion: The effects of HIIT on eIF2B, ATF4, and CHOP proteins likely induce cellular adaptations that could be an effective treatment for obese diabetic rats through the interaction of these proteins
Keywords
Diabetes
HIIT program
Obesity
Left ventricle
eIF2α
ATF4
CHOP
Subjects
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Daneshvar Medicine
Volume 32, Issue 6 - Serial Number 175
March and April 2025
Pages 41-52