Daneshvar Medicine

Daneshvar Medicine

The effect of eight weeks of contiuous aerobic training with moderate intensity on the apoptosis indices of heart tissue in aged male rats

Document Type : Original Article

Authors
Farnaz Seifi Skishahr
Sajjad Ramezani
Department of Sports Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
10.22070/daneshmed.2024.19721.1557
Abstract
Background and Objective: The aging process is characterized by a series of molecular and cellular changes over the years that can lead to the destruction of important physiological parameters to keep an organism alive and healthy. On the other hand, physical activity not only improves heart function but also reduces the risk of heart disease. Therefore, this study aimed to investigate the effect of eight weeks of continuous aerobic training (MICT) on the apoptosis indices of heart tissue in aged male rats.
Materials and Methods: In this experimental study, 16 male Wistar rats with an average age of 19±2.25 months and weight of 350.02±20.12 grams were used. They were randomly divided into two groups of moderate intensity continuous training (MT) and control (C). The continuous aerobic exercise program (MICT) was performed for 8 weeks and five sessions per week with an intensity of 60-75% of the maximum speed. 48 hours after the last training session, after complete anesthesia and killing of the mice, heart tissue was removed and immediately frozen in nitrogen solution and kept at -80 degrees. The gene expression values of BAX, BCL2, the ratio of BAX to BCL2 and P53, and caspase 3 of the heart were measured by real-time PCR. The data were analyzed by independent t-statistical method in SPSS 27 software at a significant level of P<0.05.
Results: The findings showed that eight weeks of MICT training significantly reduced the apoptotic indices of BAX (P=0.002), P53 (P=0.015), and caspase 3 (P=0.002) in the heart tissue of elderly rats compared to the control group. Also, the results showed that MICT training increased BCL2 (P=0.011) and decreased the ratio of BAX to BCL2 (P=0.008) in the heart tissue of elderly rats.
Conclusion: It seems that regular continuous aerobic exercise using the MICT method can play a significant role in reducing the apoptotic indices of the heart tissue and slowing down the aging process.
Keywords
Continuous exercise
Apoptosis
Heart
Elderly
Subjects
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Daneshvar Medicine
Volume 32, Issue 5 - Serial Number 174
January and February 2025
Pages 55-66