Daneshvar Medicine

Daneshvar Medicine

Hepatoprotective effects of caffeic acid in D-galactose-induced aging in mice: investigation of antioxidant mechanisms

Document Type : Original Article

Authors
Mir Mahdi Hosseini 1
Hossein Kalarestaghi 2
Ramin Salimnejad 2
1 School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
2 Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
10.22070/daneshmed.2024.19740.1560
Abstract
Background and Objective: The liver is a complex metabolic organ that maintains body homeostasis. Oxidative stress caused by aging is an important risk factor in the development of chronic liver diseases. This study aimed to investigate the effect of caffeic acid on oxidative damage caused by aging in mice.
Materials and Methods: In this experimental study, 40 male mice were randomly divided into 5 groups (n=8): 1) control (Con); 2) Sham; 3) caffeic acid (CA), 4) aging (Ag), and 5) aging + caffeic acid (Ag + CA). The aging model was induced through daily intraperitoneal (i.p.) injections of D-galactose (300 mg/kg) for 6 weeks. Caffeic acid (60 mg/kg, i.p.) was injected daily for 6 weeks. The mice were anesthetized 24 hours after the last injection and the liver was removed immediately. Then, oxidative stress factors (malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant capacity (TAC)) were investigated by One way ANOVA and Tukey.
Results: The results showed that D-galactose-induced aging significantly increases the level of MDA and also decreases the level of SOD, GPx, and TAC in the liver compared to the control and sham groups (P<0.05). Treatment with caffeic acid in the Ag + CA group significantly decreased MDA levels and improved the activity of SOD, GPx, and TAC (P<0.05).
Conclusion: The results showed that caffeic acid reduces lipid peroxidation and improves the liver antioxidant enzyme function.
Keywords
Caffeic acid
Aging
Liver
D-galactose
Lipid peroxidation
Subjects
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Daneshvar Medicine
Volume 32, Issue 5 - Serial Number 174
January and February 2025
Pages 1-10