The effect of eight weeks of endurance training on the contents of dynamin-related protein 1 (DRP1) and optic atrophy 1 (OPA1) related to mitochondrial fission and fusion in the left ventricle of aged rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

2 Department of Physical Education, Farhangian University, Tehran, Iran

Abstract

Background and Objective: Mitochondrial dynamics through fission and fusion play an important role in physiological and pathological conditions in aging. Disruption of mitochondrial dynamics contributes to age-related cardiovascular diseases, while exercise therapy has been proposed to prevent this process.
Materials and Methods: The current research is of an experimental type, which was conducted with 12, 20-month-old Wistar male rats with an average weight of 400 ± 30 gr. Rats were randomly divided into two endurance training and control groups (each group had 6 rats). The training group ran on the treadmill for 8 weeks and 5 sessions per week. The intensity of the endurance training was 55-75% of speed; which started with a speed of 12 meters per minute in the first week and reached a speed of 33 meters per minute in the eighth week. After 48 hours from the last training session, the rats were anesthetized and the variables were measured by western blot method. Data were analyzed through independent t-test in SPSS software version 29 and Graphpad Prism version 10.2.3 and the significance level was considered P≤0.05.
Results: Eight weeks of endurance training caused a significant increase in DRP1 content (P=0.001); While it was not significant for OPA1 protein content in the heart of old rats (P=0.16).
Conclusion: It seems that endurance training with appropriate intensity and duration can improve mitochondrial quality control in the heart tissue of elderly patients.

Keywords

References

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Daneshvar Medicine
Volume 32, Issue 4 - Serial Number 173
November and December 2024
Pages 11-22

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