Daneshvar Medicine

Daneshvar Medicine

The Effect of continuous aerobic exercise with Citrus aurantium L. on the expression of HM and MOTS-C genes in the mitochondria of the liver tissue of aged rats

Document Type : Original Article

Authors
Saba Ahmadi
Saqqa Farajtabar Behrestaq
Masoumeh Habibian
Babisan Askari
Amir Taghipour
Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
10.22070/daneshmed.2024.19122.1493
Abstract
Background and Objective: Aging process is associated with disruption in mitochondria and liver. The aim of this study was to was to evaluate the effect of continuous aerobic exercise with citrus aurantium L. on the expression of HM and MOTS-C genes in the mitochondria of the liver tissue of elderly rats.
Materials and Methods: To conduct the present experimental research, 32 elderly female rats over 14 months of age and average weight of 270 to 320 grams were divided into 4 groups of 8 including 1- Control (CN), 2- Consumption of citrus aurantium L. (CA), 3- Training (T), 4- Training and consumption of citrus aurantium L. (T+CA). During eight weeks, groups 3 and 4 ran three sessions a week with an intensity of 65-75% of the maximum running speed on the treadmill. Also, groups 2 and 4 received 300 mg/kg of CA extract intraperitoneally. 48 hours after the research protocol, gene expression levels in liver tissue were measured by real-time PCR method.
Results: Expression of HM and MOTS-C genes in CA (p=0.032 and p=0.042), T (p=0.039 and p=0.048) and T+CA (p=0.0001 and p=0.001) groups compared to CN group had a significant increase.
Conclusion: Eight weeks of T and CA alone improved the expression of genes involved in Liver cellular senescence markers of elderly rats. Also, the simultaneous effect of exercise and CA on HM was greater than the effect of each one alone.
Keywords
Training
Citrus aurantium
Mitochondria
HM
MOTS-C
Subjects
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Daneshvar Medicine
Volume 32, Issue 3 - Serial Number 172
September and October 2024
Pages 58-67