The effect of high-intensity interval training with high-fat diet on the regulation of the inflammasome complex in the adipose tissue of male rats

Document Type : Original Article

Authors

Department of Physical Education & Sport Sciences, Faculty of Humanities, Tarbiat Modares University, Tehran, Iran

Abstract

Background and Objective: Inflammasome complex causes maturation and expansion of caspase-1activity and pro-inflammatory  cytokines including IL-1β. It is also critical for inducing pyroptotic cell death in response to pathogens and endogenous danger signals. Therefore, the present study was conducted to investigate the effect of high-intensity interval training with high-fat food on the regulation of the inflammasome complex in the adipose tissue of male Wistar rats.
Materials and Methods: In the present experimental study; 24 male Wistar rats (age: six weeks); were divided into four groups: control (C), exercise (HIIT), high-fat diet (HFD), high-fat diet+exercise (HFD+HIIT). Diet and exercise interventions started at the same time and continued for 10 weeks. Weight, BMI, serum lipid profile, and also the expression of NLRP3 and IL-1β in visceral adipose tissue were measured. To determine changes between groups, one-way ANOVA and Tukey's post hoc test were used, and an independent t-test was used to determine the intensity of the effect size using SPSS27 software (P<0.05).
Results: Significant weight reduction (P=0.009), NLRP3 expression (P=0.001), IL-1β (P=0.001), and LDL and cholesterol levels (P=0.001) in the HFD+HIIT group compared to HFD were seen. Also, a significant increase in HDL and HDL/LDL was observed in the HFD+HIIT group compared to the HFD group (P=0.001).
Conclusion: The exercise training protocol is used in the current study as an effective therapeutic strategy for improving metabolic disorders and inflammatory conditions, which leads to the reduction of inflammation. However, more research is needed to determine other cellular mechanisms of inflammation.

Keywords

Main Subjects

References

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Daneshvar Medicine
Volume 32, Issue 3 - Serial Number 172
September and October 2024
Pages 46-57

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