The effect of 8-week resistance training on the content of RAS and RAF-1 proteins in the EDL muscle of old rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

2 Department of Physical Education and Sport Science, Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran

Abstract

Background and Objective: The mitogen-activated protein kinase (MAPK) pathway is one of the important pathways in the regulation of muscle volume, and aging is one of the important factors in this disorder. Therefore, the aim of this research is the effect of eight weeks of resistance training on the content of RAS and RAF-1 proteins in the extensor digitorum longus (EDL) muscle of old rats.
Materials and Methods: In this fundamental experimental research, 12 male Sprague-Dawley rats aged 20 months with an average weight of 400±30 gr were randomly divided into 2 groups:1-control (6 rats) and 2- resistance training (6 rats). The resistance training program was 3 sessions per week for 8 weeks. Rats climbed a one meter vertical ladder with an 85-degree incline, 26 rungs and two cm of space between each rung. The content of proteins was measured through Western Blot laboratory method. Data analysis was done through independent t-test in SPSS version 27 and GraphPad Prism version 10.2.2. The significance level was set at P≤ 0.05.
Results: Eight weeks of resistance training led to a significant increase in RAS and RAF-1 protein levels in the EDL muscle of aged rats (P=0.0001). Cohen's test showed a strong effect size for the amount of RAS (4.44) and RAF-1 (4.88) proteins.
Conclusion: It seems that the increase of RAS and RAF-1 proteins following resistance training can lead to the initiation of the MAPK pathway as a pathway in the regulation of muscle volume and mass.

Keywords

Main Subjects

References

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Daneshvar Medicine
Volume 32, Issue 2 - Serial Number 171
July and August 2024
Pages 63-72

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