The effect of high-intensity interval training (HIIT) on the level of mitochondrial dynamic and mitophagy proteins (FUNDC1 and NIX) in soleus muscle of male Wistar rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sport Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

2 Department of Physical Education and Sport Science, Yadegar-e-Imam Khomeini (RAH) Shahre-Rey Branch, Islamic Azad University, Tehran, Iran

Abstract

Background and Objective: The function and quality of mitochondria is very important for human life. Many pathways are involved in mitochondrial quality regulation such as mitophagy. Exercise as a physical stress can affect mitophagy; Therefore, the aim of this research was the effect of high-intensity interval training (HIIT) on the amount of mitochondrial dynamic and mitophagy proteins (FUNDC1 and NIX) in soleus muscle of male Wistar rats.
Materials and Methods: In this experimental study, 12 Wistar rats with an average weight of 280±30 were randomly divided into two groups:1.Control group, 2.HIIT (6 rats per group). The rats in the HIIT group were trained for 8 weeks, with five sessions per week, and each session consisted of 10 high-intensity intervals for 3 minutes with 2-minute rest intervals. Skeletal muscle tissue was harvested from the animals 48 hours after the last training session. The contents of the variables were measured by western blotting. Data were analysed through independent t-test in SPSS version 27 and GraphPad Prism version 10.2 software. A significance level of p≤0.05 was considered.
Results: The intracellular content of FUNDC1 protein showed a significant decrease in the HIIT group compared to the control group (P=0.0001); But this difference in NIX protein content was not significant (P=0.69).
Conclusion: The cellular regulation of these factors can mean the optimal regulation of mitophagy through exercise training such as HIIT. To clarify the effectiveness of training exercises, more studies are needed in this field.

Keywords

Main Subjects

References

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Daneshvar Medicine
Volume 32, Issue 1 - Serial Number 170
March and April 2024
Pages 70-80

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