The effectiveness of a course of endurance training and Urtica dioica hydroalcoholic extract on the stereological changes of the capillaries of the hippocampal tissue of diabetic rats

Document Type : Original Article

Authors

1 Department of Sports Physiology, Sari Branch, Islamic Azad University, Sari, Iran

2 Department of Sports Sciences, Faculty of Literature and Humanities, Lorestan University, Khorramabad, Iran

3 Motor Behavior Department, Borujerd Branch, Islamic Azad University, Borujerd, Iran

4 Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

Abstract

Background and Objective: Diabetes has harmful effects on the vascular structure and angiogenesis of various organs, including the hippocampus. In this regard, this study was designed to investigate the effect of non-pharmacological interventions of endurance training (Ex) and Urtica dioica (UD)  plant hydroalcoholic extract on the stereological changes of the hippocampal capillaries of diabetic rats.
Materials and Methods: 35 Wistar male rats were divided into 5 groups: Healthy control, Diabetes control, Diabetes+Ex, Diabetes+UD, Diabetes+Ex+UD. Diabetes was induced by intraperitoneal injection of streptozotocin. The Ex protocol with moderate intensity and daily gavage of UD hydroalcoholic extract was implemented for six weeks. 48-h after the last session, the rats was sacrificed, and then the hippocampal tissue was removed and placed in 10% formalin for hematoxylin-eosin staining. The stereological changes of the hippocampal tissue were investigated in the parameters of the number of vascular sections, length, volume and area of capillaries. Data analysis was done using repeated measures ANOVA tests for blood glucose variable and Kruskal-Wallis test for stereological studies of hippocampal tissue.
Results: Diabetes caused a significant decrease in the number of vascular sections, length, volume and area of hippocampal capillaries (P<0.001). Thus, all stereological parameters in the combined group of Ex+UD were better than the groups that implemented only one of the interventions (P<0.05). The changes of Ex and UD groups in stereological parameters of hippocampal tissue after 6 weeks were not significant.
Conclusion: The results obtained in  combination effectiveness of Ex and UD on the stereological parameters of the hippocampal tissue of diabetic rats promise to improve the health of the vessels and angiogenesis of the hippocampal tissue by changing the lifestyle of diabetic people.

Keywords

Main Subjects

References

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Daneshvar Medicine
Volume 32, Issue 1 - Serial Number 170
March and April 2024
Pages 45-58

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