Daneshvar Medicine

Daneshvar Medicine

Simultaneous effect of four weeks of Nigella sativa oil supplementation and aerobic exercise on serum levels of malondialdehyde and total antioxidant capacity in inactive young women

Document Type : Original Article

Authors
Ali Ghasemi Kahrizsangi 1
Zahra Hajikhodadadi 2
Zahra Abedin Zadeh 2
Mohsen Salehi 3
1 Sport Sciences Department, University of Qom, Qom, Iran
2 Sports Physiology Department, Qom University, Qom, Iran
3 Department of Statistics, Faculty of Science, University of Qom, Qom, Iran
10.22070/daneshmed.2024.18239.1405
Abstract
Background and Objective: Exercise interventions and consumption of some supplements can lead to changes in the antioxidant system and oxidative stress of skeletal muscles. Based on this, the present study investigated the effect of four weeks of Nigella sativa oil supplementation and aerobic exercise on the serum levels of malondialdehyde and the total antioxidant capacity of inactive young women.
Materials and Methods: For this purpose, 40 inactive young women with an average age of 22.23 ± 4.51 years and BMI of 25.46 ± 4.21 participated in the research. Prior to the treatment, they filled out a written consent form. Then, the subjects were randomly divided into four groups: control (N=9), exercise + placebo (N=10), supplement (N=10), and exercise + supplement (N=11). The aerobic exercise groups performed four weeks of aerobic exercise with an intensity of 50% to 80% of the maximum heart rate, three sessions a week. Also, the Nigella sativa oil supplement groups received 1000 mg of its capsule per day after lunch. Blood samples were collected to measure the serum level of malondialdehyde and total antioxidant capacity in two stages before and after 24 hours of the last training session in fasting conditions from antecubital vein. Statistical analysis was performed by paired t-tests and the analysis of covariance at a significance level of P<0.05.
Results: Results showed that there is a significant decrease in total antioxidant capacity values between the aerobic group with the two supplement groups (P=0.03) and supplement + exercise (P=0.03). Also, a significant decrease was shown after the test as was compared to the pre-test in the within-group values of malondialdehyde serum concentration in the supplement group (P=0.03).
Conclusion: According to the results, we conclude that the aerobic exercises and Nigella sativa supplementation leads to a decrease in the total antioxidant capacity and it has no effect on the oxidative index.
Keywords
Aerobic exercises
Malondialdehyde
Total antioxidant capacity
Nigella sativa supplement
Inactive women
  1. Mehri A, Hosseinpour Delaware S, Azizi M, Azarbaijani M A, Farzangi P. Effect of 8 weeks aerobic training and supplementation of resveratrol on oxidative marker MDA and Antioxidant SOD and GPX cardiomyocytes tissue in streptozotocin-diabetic rats. Journal of Plasma and Biomarkers 2020; 13(3):97-108.
  2. Sawada Y, Ichikawa H, Ebine N, Minamiyama Y, Alharbi AAD, Iwamoto N, et al. Effects of High-Intensity Anaerobic Exercise on the Scavenging Activity of Various Reactive Oxygen Species and Free Radicals in Athletes. Nutrients 2023;15(1):222.
  3. Hosseini Mk, Tartibiyan B. Estimation of physiological indicators in sports. Tehran: tabib, Timurzadeh 2007:192.
  4. Mastaloudis A, Morrow JD, Hopkins DW, Devaraj S, Traber MG. Antioxidant supplementation prevents exercise-induced lipid peroxidation, but not inflammation, in ultramarathon runners. Free Radical Biology and Medicine. 2004;36(10):1329-41. 10.1016/j.freeradbiomed.2004.02.069
  5. Brancaccio M, Mennitti C, Cesaro A, Fimiani F, Moscarella E, Caiazza M, et al. Dietary thiols: A potential supporting strategy against oxidative stress in heart failure and muscular damage during sports activity. International Journal of Environmental Research and Public Health. 2020;17(24):9424. 10.3390/ijerph17249424
  6. Pingitore A, Lima GPP, Mastorci F, Quinones A, Iervasi G, Vassalle C. Exercise and oxidative stress: Potential effects of antioxidant dietary strategies in sports. Nutrition. 2015;31(7-8):916-22. https://doi.org/10.1016/j.nut.2015.02.005
  7. Ye Y, Lin H, Wan M, Qiu P, Xia R, He J, et al. The effects of aerobic exercise on oxidative stress in older adults: A systematic review and meta-analysis. Frontiers in Physiology 2021;12:701151.
  8. Pangkahila EA, Adiputra N, Pangkahila W, Yasa IWPS. Balanced physical exercise increase physical fitness, optimize endorphin levels, and decrease malondialdehyde levels. Bali Medical Journal 2016;5(3):493-6.
  9. Metin G, Gumustas M, Uslu E, Belce A, Kayserilioglu A. Effect of regular training on plasma thiols, malondialdehyde and carnitine concentrations in young soccer players. Chinese Journal of Physiology 2003;46(1):35-9.
  10. Park S-Y, Kwak Y-S. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. Journal of Exercise Rehabilitation 2016;12(2):113.
  11. Vargas-Mendoza N, Angeles-Valencia M, Morales-González Á, Madrigal-Santillán EO, Morales-Martínez M, Madrigal-Bujaidar E, et al. Oxidative stress, mitochondrial function and adaptation to exercise: New perspectives in nutrition. Life 2021;11(11):1269.
  12. Alenzi FQ, Altamimi MA, Kujan O, Tarakji B, Tamimi W, Bagader O, et al. Antioxidant properties of Nigella sativa. J Mol Genet Med. 2013;7(3):1-5.
  13. Ardiana M, Pikir B, Santoso A, Hermawan H, Al-Farabi M. Effect of Nigella sativa supplementation on oxidative stress and antioxidant parameters: A meta-analysis of randomized controlled trials. The Scientific World Journal. 2020; doi.org/10.1155/2020/2390706
  14. Yousefi M, Adineh H, Reverter M, Hamidi MK, Vatnikov YA, Kulikov EV, et al. Protective effects of black seed (Nigella sativa) diet supplementation in common carp (Cyprinus carpio) against immune depression, oxidative stress and metabolism dysfunction induced by glyphosate. Fish & Shellfish Immunology 2021;109:12-9.
  15. s. Nima, Z. Farhad. Sample Size in Medical Research: an applied approach. ASRP 2017; 29-31.
  16. Ahmadian TA. The effect of 6-Week aerobic exercises with the spirulina supplementation consumption on aerobic performance in non-athletic girls. Journal of Sport and Biomotor Sciences 2016;8(15):37-43.
  17. Kaatabi H, Bamosa AO, Lebda FM, Al Elq AH, Al-Sultan AI. Favorable impact of Nigella sativa seeds on lipid profile in type 2 diabetic patients. Journal of family & community medicine 2012;19(3):155.
  18. Vakili J, Gayini A A, Hedayati M, Saeed N, Circadian changes of malondialdehyde and protein carbonyl in healthy university men. University of Medical Sciences and Health Services 2013;2(35):105-98.
  19. Gaini, Sa-IW,  K, Moqransi. The effect of endurance training and non-training on lipid peroxidation and antioxidant system of Wistar rats. Kinesiology and Sports 2008;11(6):63-51.
  20. Yusufpour M, Qasim Nian A A, Rahmani A. The effect of a period of intense intermittent training on the amount of total antioxidant capacity and malondialdehyde in the liver tissue of male Wistar rats. Scientific Journal of Kurdistan University of Medical Sciences 2018;5(22):103-10.
  21. Baghaiee B, Teixeira AB, Tartibian B. Moderate aerobic exercise increases SOD-2 gene expression and decreases leptin and malondialdehyde in middle-aged men. Science & Sports 2016;31(3):e55-e63.
  22. Hejazi M, Nizam Dost Z, Jo MS. The effect of 12 weeks of aerobic exercise on serum levels of leptin, vaspin and some indices of oxidative stress in obese middle-aged women. Iranian Journal of Endocrinology and Metabolism 2014;2(16):118-1.
  23. White A, Estrada M, Walker K, Wisnia P, Filgueira G, Valdés F, et al. Role of exercise and ascorbate on plasma antioxidant capacity in thoroughbred race horses. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 2001;128(1):99-104.
  24. Ji LL. Antioxidants and oxidative stress in exercise. Proceedings of the Society for experimental Biology and Medicine 1999;222(3):283-92.
  25. Vakili J,  Amir Sasan  R,  Hashem Pour S,  Khanvari  The effect of black seed supplement on exercise response of superoxide dismutase and serum malondialdehyde in male volleyball players. quarterly journals of applied health studies in exercise physiology. 2017;2(3):46-0.
  26. Azizibigi K, Sasan RA, Atsek S. The effect of two resistance training protocols on fat peroxidation and total plasma antioxidant capacity changes in healthy men. Sports Biology Journal 2014;3(6):245-57.
  27. Ghasemipour S, Marandi SM, Quaidi K, Nasab FK, Shirkhani S, Abdullahi M. The effect of endurance training and green coffee on oxidative stress and nitric oxide indices in pre-diabetic male rats. Applied studies of biological sciences in sports 2022. https://doi.org/10.22077/jpsbs.2022.5133.1701.
  28. Tabriz I. Effects of aerobic and exhaustive exercise on salivary and serum total antioxidant capacity and lipid peroxidation indicators in sedentary men. Feyz, Journal of Kashan University of Medical Sciences 2016;20(5):427-34.
  29. Zarrindast S, Ramezanpour M, Moghaddam M. Effects of eight weeks of moderate intensity aerobic training and training in water on DNA damage, lipid peroxidation and total antioxidant capacity in sixty years sedentary women. Science & Sports 2021;36(3):e81-e5.
  30. Mahbod E. The Impact of the Psychological Stress on the Heart Rate of the Losing and Winning Coaches of the Soccer Premier League 2019; 9(3):65-75.
  31. Moradi Z, Shameshki l, Basami M. The effect of saffron supplementation on changes in superoxide desmutase and catalase enzyme levels during a session of intense anaerobic activity in young women. Sport physiology 2012;14:130-19.
Daneshvar Medicine
Volume 31, Issue 6 - Serial Number 169
March and April 2024
Pages 14-25