Daneshvar Medicine

Daneshvar Medicine

The effect of 6-week endurance training with the consumption of nettle hydroalcoholic extract on testicular tissue apoptosis in streptozotocin-induced diabetic rats

Document Type : Original Article

Authors
Fatemeh Fatholahi
Masoud Rahmati
Rahim Mirnasouri
Hadis Mehrabi Fard,
Exercise Physiology Department, Faculty of Humanity Sciences, Lorestan University, Lorestan, Iran
10.22070/daneshmed.2024.18442.1423
Abstract
Background and Objective: Diabetes is a metabolic disease that is associated with infertility disorders and testicular tissue apoptosis. The present study was conducted with the aim of investigating the effect of 6 weeks of endurance training along with the consumption of nettle hydroalcoholic extract on the apoptosis of the testicular tissue of diabetic rats.
Materials and Methods: 58 male Wistar rats were randomly divided into five groups: "healthy control", "control diabetes", "exercise diabetes", "supplementary diabetes" and "supplementary exercise diabetes". Diabetes was induced by intraperitoneal injection of 45 mg/kg STZ dissolved in fresh citrate buffer. Nettle plant extract was fed to the animals for 6 weeks. The endurance training protocol was performed for 6 weeks and 48 hours after the last training session, the rats were dissected and their testicular tissue was extracted. Tunnel staining method was used to check the apoptosis of testicular tissue. The results of the Kolmogorov Smirnov and Lunn tests were used to check the normality of the data and the homogeneity of the variances. Also, one-way analysis of variance (Tukey's post hoc test) was used to compare groups for the studied variables.
Results: Data analysis showed that apoptosis was significantly reduced in the "exercise diabetes", "supplementary diabetes" and "supplementary exercise diabetes" groups compared to the "control diabetes" group (P = 0.001). Nettle plant extract and endurance exercise in diabetic rats reduced testicular tissue apoptosis.
Conclusion: Consumption of nettle hydroalcoholic extract along with endurance exercise can reduce the apoptosis of testicular tissue in diabetic rats. Therefore, it is suggested to have exercise as a non-pharmacological intervention and nettle plant, which is a non-chemical intervention, to reduce the complications caused by diabetes in the field of fertility.
Keywords
Endurance Training
Nettle hydroalcoholic extract
Apoptosis
Type 1 diabetes
Subjects
  1. Ansari S, Djalali M, Mohammadzadeh Honarvar N, Mazaherioun M, Zarei M, Gholampour Z. Assessing the effect of omega-3 fatty acids supplementation on serum BDNF (brain derived neurotrophic factor) in patients with type 2 diabetes:a randomized, double-blind, placebo-controlled study. International Research Journal of Applied and Basic Sciences 2016;4(10):380-3.
  2. Nobahar M, Asgari M, Baba Mohammadi H, Soleymani M. [Book comprehensive Text Book of Medical Surgical Nursing ] 2nd ed. Tehran-Hakim Hidajy(Boshra) 2011:854-5.
  3. Pishdad GR. Low incidence of type 1 diabetes in Iran. Diabetes Care 2005;28(4):927-9. PMID: 15793198 DOI: 10.2337/diacare.28.4.927
  4. Dinh T, Veve A. Microcirculation of the diabetic foot. Current Pharmaceutical Design 2005; 11(18): 2301-2309.
  5. Ahmadpour SH, Sadeghi Y. Effect of insulin and ascrobie acid therapy on plasma cu level in STZ-induced diabetic rats. Journal of Nutrition Science and Vitaminology 2008;4(54):321-8.
  6. Figueroa Romero C, Sadidi M, Feldman EL. Mechanisms of disease the oxidative stress theory of diabetic neuropathy. Journal of Nutrition Science and Vitaminlogy 2008;4(54):213-8.
  7. Kumar N, Singh A. Trends of male factor infertility, an important cause of infertility: A review of literature. Journal of Human Reproductive Sciences 2015;4(8):191-6.
  8. De A, Singh M, Singh V, Ram V, Bisht S. Treatment effect of l-Norvaline on the sexual performance of male rats with streptozotocin induced diabetes. European Journal of Pharmacology 2016(771):247-54.
  9. Ghanbari E, Nejati V, Najafi G, Khazaei M, Babaei M. Study on the effect of royal jelly on reproductive parameters in streptozotocin-induced diabetic rats. International Journal of Fertility & Sterility. 2015;1(9):113-20.
  10. Ghanbari E, Nejati V, Khazaei M. Antioxidant and protective effects of Royal jelly on histopathological changes in testis of diabetic rats. International Journal of Reproductive BioMedicine 2016;8(14):519.
  11. Favaloro B, Allocati N, Graziano V, Di Ilio C, De Laurenzi V. Role of apoptosis in disease. Aging (Albany NY) 2012;4(5):330-49. doi: 10.18632/aging.100459. PMID: 22683550; PMCID: PMC3384434.
  12. McMillan E, Graham D, Rush J, Quadrilatero J. Decreased DNA fragmentation and apoptotic signaling in soleus muscle of hypertensive rats following 6 weeks of treadmill training. Journal of Applied Physiology 2012;7(113):1048-57.
  13. Quadrilatero J, Bombardier E, Norris S, Talanian J, Palmer M, Logan H, et al. Prolonged moderate-intensity aerobic exercise does not alter apoptotic signaling and DNA fragmentation in human skeletal muscle. American Journal of Physiology-Endocrinology and Metabolism 2010;3(298):E534-E47.
  14. Siu PM. Muscle apoptotic response to denervation, disuse, and aging. Medicine and Science in Sports and Exercise. 2009 Oct;41(10):1876-1886. DOI: 10.1249/mss.0b013e3181a6470b. PMID: 19727026.
  15. Offen D, Elkon H, Melamed E. Apoptosis as a general cell death pathway in neurodegenerative diseases. Journal of Neural Transmission. Supplementa 2000(21):153-66. PMID: 11128605 DOI: 10.1007/978-3-7091-6284-2_13
  16. Khademi Z, Imani E, HeidaryKhormizi M, PoordadKhodaei A, Sarneyzadeh M, Nikparvar M. A Study on The Variation of Medicinal Plants Used for Controlling Blood Sugar and Causes of Self Medication by Patients Referred to Bandarabbas Diabetic Center. Journal of Diabetes Nursing 2013;1(1):12-20.
  17. Namazi N, Bahrami A. Effect of Hydroalcoholic Nettle Extract on Lipid Profiles and Blood Pressure in Type 2 Diabetes Patients. Iran J Endocrinol Metabol 2012;5(13):449-58.
  18. Hunt L, Arar N, Akana  L. Herbs, prayer, and insulin: Use of medical and alternative treatments by a group of Mexican-American diabetic patients. J Fam Pract. 2000(49):216-23.
  19. Zargari A. Medicinal plants, 6th ed . Tehran University of Medical Sciences. 1375:401-9.
  20. Kavalali G, Tuncel H, Goksel S, Hatemi H. Hypoglycemic activity of Urtica pilulifera in streptozotocin-diabetic rats  Ethanopharmacol. Journal of Ethnopharmacology 2003;(84) 241-245.
  21. Emmelin N, Feldberg W. Distribution of acetylcholine and histamine in nettle plants. New Phytol 1949(48):143-8.
  22. Wagner H, Willer  F, Samtleben R, Boos G. Search for the antiprostatic principle of stinging nettle (Urtica dioica) roots. Phytomedicine. Volume 1, Issue 3, December 1994, Pages 213-224.
  23. Hasani-Ranjbar S, Larijani B, Abdollahi M. A systematic review of Iranian medicinal plants useful in diabetes mellitus. Archives of Medical Science 2008;3(4):285-292.
  24. Fakhraee S, Jouyandeh Z, Mehri A, Larijani B, Hasaniranjbar S. Systematic review on the effectiveness and safety of nettle herb in treating diabetes. Iranian Journal of Diabetes and Lipid Disorders 2012;12(6):507-23.
  25. Rezaeiaref TM, Latifpour M. Protective Effects of Urtica Dioica Extract on the Damage of Rat Small Intestinal Mucosa Caused by Diabetes. Journal of Babol University of Medical Sciences 2012;14(3):31-7.
  26. Hasani AE, Ramezanpoor M. The effect of 8 weeks of regular aerobic exercise and nettle extract consumption on glucose level and insulin resistance index in type 2 diabetic. women Journal of Knowledge and Health 2016;4(10):6-11.
  27. Praet S, Van Loon L. Optimizing the therapeutic benefits of exercise in type 2 diabetes. Ournal of Applied Physiology 2007;103(4):1113-20.
  28. Suzanne CS, Bare B, Janice LH, Kerry H. [Cheever Brunner and Suddarth’s Textbook of Medical-Surgical Nursing (Two Volume Set)] 12th ed. Tehran-Jame enegar&Salemi 2010:155-66.
  29. Gharakhanlou R, Chadan S, Gardiner P. Increased activity in the form of endurance training increases calcitonin gene-related peptide content in lumbar motoneuron cell bodies and in sciatic nerve in the Rat. Neuroscience 1999(89):1229-39.
  30. Kim D, Ko I, Kim B, Kim T, Kim S, Shin M, et al. Treadmill exercise inhibits traumatic brain injuryinduced hippocampal apoptosis. Physiol Behav 2010(101):660-5.
  31. Mazzola P, Terra M, Rosa A, Mescka C, Moraes T, Piccoli B, et al. Regular exercise prevents oxidative stress in the brain of hyperphenylalaninemic rats.Metabolic Brain Disease 2011(26):291-7.
  32. Cheng SM, Ho TJ, Yang AL, Chen IJ, Kao CL, Wu FN, et al . Exercise training enhances cardiac IGFI-R/PI3K/Akt and Bcl-2 family associated pro-survival pathways in streptozotocin-induced diabetic rats. International journal of cardiology. 2013 Jul 31;167(2):478-85.
  33. Dakhili A, Gharakhanlou R, Movaheddin M, Khazani A, Keshavarz M. The Effect Of 6 Weeks Endurance Training On Gene Expression Of Nerve Growth Factor In Sensory Spinal Cord Of Rats With Diabetic Neuropathy. Iranian Journal of Diabetes and Lipid Disorders 2014; 13 (3): 263-271.
  34. Avendano C, Franchi A, Duran H, Oehninger S. DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome. Fertility and Sterility 2010;94(2):549-57.
  35. Wyllie A. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature Reviews Genetics 1980;284(5756):555-6.
  36. Elmore S. Apoptosis: a review of programmed cell death. Toxicologic pathology. 2007 Jun;35(4):495-516.
  37. Qiao X, Chen X, Wu  D, Ding R, Wang J, Hong Q, et al. Mitochondrial pathway is responsible for aging-related increase of tubular cell apoptosis in renal ischemia/reperfusion injury. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2005;60(7):830-9.
  38. Ashkenazi A, Dixit VM. Death receptors: signaling and modulation. science. 1998 Aug 28;281(5381):1305-8.
  39. Mirdar Harijani S, Musavi N, et al. Effect of Endurance Swimming Training and SilymarinTreatment on Changes in Liver Apoptotic Index inPregnant Rats Exposed to Cadmium. Journal Mediine university Rafssanjan. 2014;13(8):705-14.
  40. Su SH, Jen CJ, Chen HI. NO signaling in exercise training-induced anti-apoptotic effects in human neutrophils. Biochemical and Biophysical Research Communications. 2011 Feb 4;405(1):58-63.
  41. Santana ET, Serra AJ, Silva Junior JA, Bocalini DS, Barauna VG, Krieger JE, et al. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Revista de Educação Física. 2014 Apr;20:233-8.
  42. Doustar Y, Mohajeri D, Rezaei A, Hashemi M. The effect of endurance swimming exercise on the occurrence of apoptosis in experimental diabetic myopathic rats. Journal of Islamic Azad Uni Tabriz Branch. 2010;3(4):629-36.
  43. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene Cell Tissue. The Official Journal of Zahedan University of Medical Sciences 2015;4(2).
  44. Peterson J, Bryner R, Sindler A, Frisbee J, Alway S. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. Journal of Applied Physiology 2008;105(6):1934-43.
  45. Koçtürk  S, Kayatekin B, Resmi H, Açıkgöz O, Kaynak C, Özer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats. European journal of applied physiology. 2008;102(5):515-24.
  46. Phaneuf S, Leeuwenburgh C. Apoptosis and exercise. Medicine & Science in Sports & Exercise 2001 Mar 1;33(3):393-6.
  47. Liu W, He W, Li H. Exhaustive training increases uncoupling protein 2 expression and decreases Bcl-2/Bax ratio in rat skeletal muscle. Oxidative medicine and cellular longevity. 2013. Article ID 780719 | https://doi.org/10.1155/2013/780719
  48. Kalyani RR, Corriere M, Ferrucci L. Age-related and disease-related muscle loss: the effect of diabetes, obesity, and other diseases. The lancet Diabetes & endocrinology. 2014 ;2(10):819-29.
  49. Guariguata L. By the numbers: new estimates from the IDF Diabetes Atlas Update for 2012. Diabetes research and clinical practice. 2012 ;98(3):524-5.
  50. Kwak HB. Effects of aging and exercise training on apoptosis in the heart. Journal of Exercise Rehabilitation 2013;9(2):212.
  51. Quindry J, French J, Hamilton K, Lee Y, Mehta JL, Powers S. Exercise training provides cardioprotection against ischemia–reperfusion induced apoptosis in young and old animals. Experimental Gerontology 2005 ;40(5):416-25.
  52. Farkhaie F, Hosseini S, Edalat-Manesh M. The effect of endurance training with niglla sativa extract on lipid profile of diabetic rats. The Journal of Nutrition, Fasting and Health 2013;14(1):11-8.
  53. Shahrjerdy SH, Shavandi N, Golpayegani M, Sheikh Hosseini R. Impact strength and resistance training on glycemic control, quality of life and mental health in women with type two diabetes. Iran J Diabetes Lipid Disord. 2009;9:35-44.
  54. Ryan EA, Pick ME, Marceau C. Use of alternative medicines in diabetes mellitus. Diabetic Medicine 2001;18(3):242-5
Daneshvar Medicine
Volume 31, Issue 5 - Serial Number 168
January and February 2024
Pages 92-103