Daneshvar Medicine

Daneshvar Medicine

Comparison of the effect of 6-week aerobic and high intensity interval training on expression of uPA and uPAR in the tumor tissue of female mice with breast cancer

Document Type : Original Article

Authors
Gita Fardad 1
Abasali Gaeini 2
Reza Nouri 2
Parisa Pournemati 2
1 Department of Sport Sciences, University of Tehran, Kish International Campus, Kish, Iran
2 Department of Exercise Physiology, Faculty of Sport Sciences and Health, University of Tehran, Tehran, Iran
10.22070/daneshmed.2024.18022.1381
Abstract
Background and Objective: In the past few decades, research on the role of physical activity in the prevention of cancer and disease progression has been the subject of attention of many researchers. The aim of this study was to compare the effect of 6 weeks of aerobic training and high intensity interval training on the expression of uPA and uPAR in the tumor tissue of female mice with breast cancer.
Materials and Methods: 24 female Balb c mice with breast cancer were randomly divided into 3 groups: tumor (T), tumor + aerobic training (T+AE) and tumor + high intensity interval training (T+HIIT). The T+AE group had incremental endurance treadmill running for 6 weeks/5 days/60 minutes. The T+HIIT group had a 6-week/3-day/60 min high intensity interval training protocol. Then, the mice were killed, the tumor tissue was removed and frozen in liquid nitrogen. The expression of uPA and uPAR genes was measured by Real Time-PCR method. ΔCT, ΔΔCT, fold change, one-way ANOVA and Tukey's tests were performed at a significant level P<0.05 with GENEX software .
Results: The results of the present study showed that in the T+AE group compared to the T group, the expression of uPA and uPAR decreased (P=0.00) 2.07 and 2.55 times. In the T+HIIT group compared to the T group, the expression of uPA and uPAR decreased 4.25 and 7.74 times (P=0.00), and in the T+HIIT group compared to the T+AE group, the expression of uPA and uPAR decreased 2.04 and 3.03 times (P=0.00).
Conclusion: Aerobic training and high intensity interval training are probably effective in preventing the progression of breast cancer by reducing the expression of uPA and uPAR.
Keywords
Breast cancer
Aerobic exercise
High intensity interval training
Urokinase-type plasminogen activator
Urokinase-type plasminogen activator receptor
Subjects
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Daneshvar Medicine
Volume 31, Issue 5 - Serial Number 168
January and February 2024
Pages 23-34