The effect of Urtica dioica in combination with endurance training on testicular spermatogenic factors in streptozotocin-induced diabetic rats

Document Type : Original Article

Authors

1 Department of Physical Education and Sport Sciences, Faculty of Literature and Human Sciences, Lorestan University, Lorestan, Iran

2 Department of Anatomical Sciences, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

Abstract

Background and Objective: Today, diabetes is a major health problem with an increasing prevalence in men. It has been stated that high glucose leads to increased oxidative stress and apoptosis in testicular cells and causes testicular cell death and infertility. This study was conducted to investigate and determine the effect of nettle hydroalcoholic extract and endurance exercises on testicular tissue and spermatogenesis factors in streptozotocin (STZ)-diabetic male rats.
Materials and Methods: In this experimental study, 40 albino rats weighing 200-220 grams were randomly divided into 5 groups: control-healthy (HC), nettle-diabetes (DG), exercise-diabetes (DT, nettle-exercise), diabetes (DTG), and control diabetes (DC). Endurance training was performed for 6 weeks according to the protocol. At the same time, rats of DG and DTG groups received nettle hydroalcoholic extract at a dose of 45 mg per kg of body weight. After 6 weeks, the testes were removed and stained with H&E technique.
Results: In the DTG and the DG groups, both spermatogenesis index (SI) and repopulation index (RI) were higher compared to the DT group and were close to the results of the healthy control group. Also, the histopathology of the testis in these two groups showed a higher improvement compared to the DT group and similar results were obtained with the results of the HC group.
Conclusion: The present study showed that diabetes has destructive effects on spermatogenesis and testicular tissue and administration of nettle and endurance exercises together can protect spermatogenic cells in seminiferous tubules from the harmful effects of diabetes by improving SI and RI percentages.

Keywords

Main Subjects

References

  1. Glovaci D, Fan W, Wong ND. Epidemiology of diabetes mellitus and cardiovascular disease. Current Cardiology Reports 2019;21:1-8.
  2. Moin ASM, Butler AE. Alterations in beta cell identity in type 1 and type 2 diabetes. Current diabetes Reports 2019;19:1-12.
  3. Papachristoforou E, Lambadiari V, Maratou E, Makrilakis K. Association of glycemic indices (hyperglycemia, glucose variability, and hypoglycemia) with oxidative stress and diabetic complications. Journal of Diabetes Research 2020:7489795. doi: 10.1155/2020/7489795.
  4. Yang B-Y, Markevych I, Heinrich J, Bowatte G, Bloom MS, Guo Y, et al. Associations of greenness with diabetes mellitus and glucose-homeostasis markers: The 33 Communities Chinese Health Study. International Journal of Hygiene and Environmental Health 2019;222(2):283-90.
  5. Alam S, Hasan MK, Neaz S, Hussain N, Hossain MF, Rahman T. Diabetes Mellitus: insights from epidemiology, biochemistry, risk factors, diagnosis, complications and comprehensive management. Diabetology 2021;2(2):36-50.
  6. Zheng S, Huang K, Tong T. Efficacy and mechanisms of oleuropein in mitigating diabetes and diabetes complications. Journal of Agricultural and Food Chemistry 2021;69(22):6145-55.
  7. Barkabi-Zanjani S, Ghorbanzadeh V, Aslani M, Ghalibafsabbaghi A, Chodari L. Diabetes mellitus and the impairment of male reproductive function: Possible signaling pathways. Diabetes & Metabolic Syndrome: Clinical Research & Reviews 2020;14(5):1307-14.
  8. Ren H, Hu K. Inflammatory and oxidative stress‑associated factors in chronic intermittent hypoxia in Chinese patients, rats, lymphocytes and endotheliocytes. Molecular Medicine Reports 2017;16(6):8092-102.
  9. Zhu X, Guo F, Tang H, Huang C, Xie G, Huang T, et al. Islet transplantation attenuating testicular injury in type 1 diabetic rats is associated with suppression of oxidative stress and inflammation via Nrf-2/HO-1 and NF-κB Pathways. Journal of diabetes Research 2019: 8712492.
  10. Esmaeili V, Shahverdi A, Moghadasian M, Alizadeh A. Dietary fatty acids affect semen quality: a review. Andrology 2015;3(3):450-61.
  11. Potashnik H, Elhija MA, Lunenfeld E, Potashnik G, Schlatt S, Nieschlag E, et al. Interleukin-6 expression during normal maturation of the mouse testis. European cytokine Network 2005;16(2):161-5.
  12. Hong CY, Park JH, Ahn RS, Im SY, Choi H-S, Soh J, et al. Molecular mechanism of suppression of testicular steroidogenesis by proinflammatory cytokine tumor necrosis factor alpha. Molecular and Cellular Biology 2004;24(7):2593-604.
  13. Rashid K, Sil PC. Curcumin enhances recovery of pancreatic islets from cellular stress induced inflammation and apoptosis in diabetic rats. Toxicology and Applied Pharmacology 2015;282(3):297-310.
  14. Tran N, Pham B, Le L. Bioactive compounds in anti-diabetic plants: From herbal medicine to modern drug discovery. Biology 2020;9(9):252.
  15. Sarani Maram Y, Vahidian-Rezazadeh M, Fanaei H. The interactive effects of resistance, endurance trainings and consumption of nettle extract on plasma apelin levels and weight changes in diabetic type 1 rats. Journal of Practical Studies of Biosciences in Sport 2020;8(16):72-84.
  16. Grauso L, de Falco B, Lanzotti V, Motti R. Stinging nettle, Urtica dioica L.: Botanical, phytochemical and pharmacological overview. Phytochemistry Reviews 2020;19:1341-77.
  17. Matos B, Howl J, Ferreira R, Fardilha M. Exploring the effect of exercise training on testicular function. European Journal of Applied Physiology 2019;119:1-8.
  18. Silva JV, Santiago J, Matos B, Henriques MC, Patrício D, Martins AD, et al. Effects of Age and Lifelong Moderate-Intensity Exercise Training on Rats’ Testicular Function. International Journal of Molecular Sciences 2022;23(19):11619.
  19. Belladelli F, Basran S, Eisenberg ML. Male Fertility and Physical Exercise. The World Journal of Men's Health 2023 ;41(3):482-488. doi: 10.5534/wjmh.220199.
  20. Shaikh H, Shrivastava VK, Amir M. Diabetes mellitus and impairment of male reproductive function: role of hypothalamus pituitary testicular axis and reactive oxygen species. Iranian Journal of Diabetes and Obesity 2016;8(1):41-50.
  21. Del Buey MA, Casas P, Caramello C, Lopez N, de la Rica M, Subiron AB, et al. An update on corneal biomechanics and architecture in diabetes. Journal of Ophthalmology 2019: 7645352.
  22. Andlib N, Sajad M, Kumar R, Thakur SC. Abnormalities in sex hormones and sexual dysfunction in males with diabetes mellitus: A mechanistic insight. Acta Histochemica 2023;125(1):151974.
  23. Ahi S, Ebrahimi F, Abedi HA, Kargar Jahromi H, Zarei S. The Effects of Hydroalcoholic Extract of Silk Cocoon on Hypothalamic-Pituitary–Gonadal Axis in Streptozotocin-Induced Diabetic Male Rats. Autoimmune Diseases Volume 2022 | Article ID 7916159 | https://doi.org/10.1155/2022/7916159.
  24. Adedara IA, Okpara ES, Busari EO, Omole O, Owumi SE, Farombi EO. Dietary protocatechuic acid abrogates male reproductive dysfunction in streptozotocin-induced diabetic rats via suppression of oxidative damage, inflammation and caspase-3 activity. European Journal of Pharmacology 2019;849:30-42.
  25. SaÇMaozu N, Eyison HM, Cebesoy S. Histomorphological changes on the testicular tissue in diabetic rats induced with streptozotocin. Communications Faculty of Sciences University of Ankara Series C Biology 2019;28(1):101-13.
  26. Begić S, Horozić E, Alibašić H, Bjelić E, Seferović S, Kozarević EC, et al. Antioxidant capacity and total phenolic and flavonoid contents of methanolic extracts of Urtica dioica L. by different extraction techniques. International Research Journal of Pure and Applied Chemistry 2020;21:207-14.
  27. Golalipour MJ, Balajadeh BK, Ghafari S, Azarhosh R, Khori V. Protective effect of Urtica dioica L.(Urticaceae) on morphometric and morphologic alterations of seminiferous tubules in STZ diabetic rats. Iranian Journal of basic Medical Sciences 2011;14(5):472.
  28. Jalili C, Salahshoor MR, Naseri A. Protective effect of Urtica dioica L against nicotine-induced damage on sperm parameters, testosterone and testis tissue in mice. Iranian Journal of Reproductive Medicine 2014;12(6):401.
  29. Mohammadi F, Nikzad H, Taherian A, Amini Mahabadi J, Salehi M. Effects of herbal medicine on male infertility. Anatomical Sciences Journal 2013;10(4):3-16.
  30. Martin LJ, Touaibia M. Improvement of testicular steroidogenesis using flavonoids and isoflavonoids for prevention of late-onset male hypogonadism. Antioxidants 2020;9(3):237.
  31. Owumi SE, Adedara IA, Akomolafe AP, Farombi EO, Oyelere AK. Gallic acid enhances reproductive function by modulating oxido-inflammatory and apoptosis mediators in rats exposed to aflatoxin-B1. Experimental Biology and Medicine 2020;245(12):1016-28.
  32. Ujah G, Nna V, Agah M, Omue L, Leku C, Osim E. Effect of quercetin on cadmium chloride‐induced impairments in sexual behaviour and steroidogenesis in male Wistar rats. Andrologia 2018;50(2):e12866.
  33. Abarikwu SO, Farombi EO. Quercetin ameliorates atrazine-induced changes in the testicular function of rats. Toxicology and Industrial Health 2016;32(7):1278-85.
  34. Abdelkader NF, Eitah HE, Maklad YA, Gamaleldin AA, Badawi MA, Kenawy SA. New combination therapy of gliclazide and quercetin for protection against STZ-induced diabetic rats. Life sciences 2020;247:117458.
  35. Asadi N, Bahmani M, Kheradmand A, Rafieian-Kopaei M. The impact of oxidative stress on testicular function and the role of antioxidants in improving it: a review. Journal of Clinical and Diagnostic Research 2017;11(5):IE01.
  36. Ye R-J, Yang J-M, Hai D-M, Liu N, Ma L, Lan X-B, et al. Interplay between male reproductive system dysfunction and the therapeutic effect of flavonoids. Fitoterapia 2020;147:104756.
  37. Hosseinabadi F, Faraji T, Malmir M. Impact of Quercetin on Sperm Parameters, Testicular Tissue and Sex Hormone: a Systematic Review. Jorjani Biomedicine Journal 2021;9(4):33-54.
  38. Minas A, Fernandes ACC, Maciel Junior VL, Adami L, Intasqui P, Bertolla RP. Influence of physical activity on male fertility. Andrologia 2022;54(7):e14433.
  39. Powers SK, Deminice R, Ozdemir M, Yoshihara T, Bomkamp MP, Hyatt H. Exercise-induced oxidative stress: Friend or ournal of Sport and Health Science 2020;9(5):415-25.
  40. Sansone A, Sansone M, Vaamonde D, Sgrò P, Salzano C, Romanelli F, et al. Sport, doping and male fertility. Reproductive Biology and Endocrinology 2018;16(1):1-12.
  41. Alahmar AT. Role of oxidative stress in male infertility: an updated review. Journal of Human Reproductive Sciences 2019;12(1):4.
Daneshvar Medicine
Volume 31, Issue 5 - Serial Number 168
January and February 2024
Pages 1-11

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