Daneshvar Medicine

Daneshvar Medicine

Ameliorative effect of ellagic acid against diabetes-induced memory deficit in rats: the role of oxidative stress

Document Type : Original Article

Authors
Safoura Raoufi
Abdolrahman Sarihi
Alireza Komaki
Siamak Shahidi
Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
10.22070/daneshmed.2023.17657.1346
Abstract
Background and Objective: Diabetes mellitus is associated with memory impairment. Ellagic acid (EA) is a natural polyphenol with anti-diabetic and neuroprotective effects. The aim of the present study was to investigate the protective effect of ellagic acid on diabetes induced memory impairment and to evaluate the role of its antioxidant effect.
Materials and Methods: Male Wistar rats were randomly divided into four groups (n=8): control, diabetic, diabetic receiving 25 mg/kg of EA, and diabetic receiving 50 mg/kg of EA. Diabetes was induced by a single intraperitoneal injection of 60 mg/kg of streptozotocin (STZ). After receiving EA (once a day) by oral gavage for four weeks, passive avoidance learning (PAL) test was used to evaluate memory. Finally, oxidative stress related factors including malondialdehyde (MDA) and total antioxidant capacity (TAC) were evaluated in the hippocampal tissue separated from the animal brains under deep anesthesia.
Results: Diabetic animals showed marked reductions in step-through latency (STLr) and elevation in time spent in the dark compartment (TDC)  in the retention trial and elevation of MDA level and diminution of TAC compared to controls significantly. Treatment of diabetic rats with EA markedly increased STLr, decreased TDC and enhanced TAC (by both EA doses) and reduced MDA (at a dose of 50 mg/kg of EA).
Conclusion: The results displayed learning and memory improving effect of EA in diabetic rats via attenuating oxidative stress.
Keywords
Diabetes mellitus
Ellagic Acid
Memory
Oxidative stress
Streptozotocin
Subjects
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Daneshvar Medicine
Volume 31, Issue 3 - Serial Number 166
September and October 2023
Pages 1-13