Daneshvar Medicine

Daneshvar Medicine

Evaluation of two types of IFN-1 induced gene expression (ly6e and usp18) and its association with the severity of clinical symptoms in COVID-19 patients

Document Type : Original Article

Authors
Umoulbanin Alibakhshi 1
Sara Ghaffarpour 2
Mohammad Mahdi Naghizadeh 3
Tooba Ghazanfari 2
1 Department of immunology, Faculty of Medicine, Shahed University, Tehran, Iran
2 Immunoregulation Research Center, Shahed University, Tehran, Iran
3 Non-communicable Diseases Research Center, Fasa University of Medical Science, Fasa, Iran
10.22070/daneshmed.2023.16760.1268
Abstract
Background and Objective: Covid-19 disease as a pandemic with high spreading power and a wide range of clinical symptoms and lack of definitive treatment is a challenge for mankind in recent years. The antiviral response of type 1 interferon against the SARS-COV-2 virus is one of the most important pathways of the innate immune system in dealing with this disease. The purpose of this study was to investigate the relationship between the expression of two genes, ly6e and usp18, with the severity of clinical symptoms in patients with covid-19.
Materials and Methods: Peripheral blood samples were prepared from patients and their DNA was extracted, then the expression of ly6e and usp18 genes was measured by real-time PCR method. cDNA samples were first amplified using specific primers. Data analysis was performed using chi-square, Kruskal-Wallis and Mann-Whitney tests. A significance level of 0.05 was considered.
Results: The expression of the ly6e gene was significantly (p=0.008) decreased in the hospitalized group compared to the control group. No significant difference was seen in the expression of the usp18 gene among the studied groups.
Conclusion: Proper expression of ly6e protein can be related to controlling the severity of clinical symptoms of covid-19 disease.
 
Keywords
covid-19
severity of clinical symptoms
type one interferon
ly6e
usp18
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Daneshvar Medicine
Volume 31, Issue 1 - Serial Number 164
May and June 2023
Pages 1-12