Daneshvar Medicine

Daneshvar Medicine

Alterations in miR-128-1 and some blood lipoproteins following 12 weeks of concurrent aerobic and strength training in obese middle-aged men

Document Type : Original Article

Authors
Amir Shakib
Ramin Amirsasan
Javad Vakili
Vahid Sari-Saraf
Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Tabriz, Tabriz, Iran
10.22070/daneshmed.2023.16835.1279
Abstract
Background and Objective: Obesity can disrupt health by causing various diseases and especially by causing disturbances in blood lipid profiles. Since very few studies have been done in relation to miRNAs related to obesity and their role in causing disturbances in blood lipoproteins, therefore the aim of this research was the alterations in miR-128-1 and blood lipoproteins following 12 weeks of concurrent aerobic and strength training (CAST) in obese middle-aged men.
Materials and Methods: 30 middle-aged men (40-50 years old) and obese (BMI≥30) randomly and based on fat percentage, aerobic capacity (VO2max) and one repetition maximum (1-RM) were assigned to three groups 1-CAST in one day 2-CAST on different days and       3-control group. Blood samples were collected from the subjects 48 hours before and after 12 weeks of concurrent training, in order to analyze miR-128-1 indices and blood lipid profiles. One-way repeated measures ANOVA and paired sample t-test were used to analyze the data.
Results: The results showed that after 12 weeks of CAST training, both training groups experienced a significant decrease in miR-128-1, HDL-C, LDL-C, triglyceride and cholesterol indices compared to the control group (P<0/05).
Conclusion: It seems that CAST training can improve lipid profiles by affecting obesity-related miRNAs, especially miR-128-1, and thereby help improve health.
Keywords
Concurrent Training
Obesity
Mir-128-1
Lipoprotein
  1. Vekic J, Zeljkovic A, Stefanovic A, Jelic-Ivanovic Z, Spasojevic-Kalimanovska V. Obesity and dyslipidemia. Metabolism 2019 ;92:71-81.
  2. Agbu P, Carthew RW. MicroRNA-mediated regulation of glucose and lipid metabolism. Nature Reviews Molecular Cell Biology 2021;22(6):425-38.
  3. Su X, Nie M, Zhang G, Wang B. MicroRNA in cardio-metabolic disorders. Clinica Chimica Acta. 2021;518:134-41.
  4. Ji C, Guo X. The clinical potential of circulating microRNAs in obesity. Nature Reviews Endocrinology 2019;15(12):731-43.
  5. Ehtesham N, Shahrbanian S, Valadiathar M, Mowla SJ. Modulations of obesity-related microRNAs after exercise intervention: a systematic review and bioinformatics analysis. Molecular Biology Reports 2021;48(3):2817-31.
  6. Eklund D, Häkkinen A, Laukkanen JA, Balandzic M, Nyman K, Häkkinen K. Fitness, body composition and blood lipids following 3 concurrent strength and endurance training modes. Applied Physiology, Nutrition, and Metabolism 2016;41(7):767-74.
  7. Schumann M, Rønnestad BR, editors. Concurrent aerobic and strength training: Scientific basics and practical applications. Springer 2018.
  8. Donghui T, Shuang B, Xulong L, Meng Y, Yujing G, Yujie H, Juan L, Dongsheng Y. Improvement of microvascular endothelial dysfunction induced by exercise and diet is associated with microRNA-126 in obese adolescents. Microvascular Research 2019;123:86-91.
  9. Gibson AL, Wagner D, Heyward V. Advanced fitness assessment and exercise prescription, 8E. Human kinetics; 2019.
  10. Bompa TO, Buzzichelli C. Periodization-: theory and methodology of training. Human kinetics books
  11. Bompa T, Buzzichelli C. Periodization training for sports, 3e. Human kinetics books 2015.
  12. Pfaffl MW. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Research 2001;29(9): e45-.
  13. Fernández-Tussy P, Ruz-Maldonado I, Fernández-Hernando C. MicroRNAs and circular RNAs in lipoprotein metabolism. Current Atherosclerosis Reports 2021;23(7):1-1.
  14. Luo J, Yang H, Song BL. Mechanisms and regulation of cholesterol homeostasis. Nature Reviews Molecular Cell Biology 2020;21(4):225-45.
  15. Wagschal A, Najafi-Shoushtari SH, Wang L, Goedeke L, Sinha S, Delemos AS, Black JC, Ramírez CM, Li Y, Tewhey R, Hatoum I. Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nature Medicine 2015;21(11):1290-7.
  16. Zhang W, Yao C, Wei Z, Dong Q. miR-128 promoted adipogenic differentiation and inhibited osteogenic differentiation of human mesenchymal stem cells by suppression of VEGF pathway. Journal of Receptors and Signal Transduction 2017;37(3):217-23.
  17. Methenitis S. A brief review on concurrent training: from laboratory to the field. Sports 2018;6(4):127.
  18. Russo A, Bartolini D, Mensà E, Torquato P, Albertini MC, Olivieri F, Testa R, Rossi S, Piroddi M, Cruciani G, De Feo P. Physical activity modulates the overexpression of the inflammatory miR‐146a‐5p in obese patients. IUBMB Life 2018;70(10):1012-22.
  19. Wang S, Liao J, Huang J, Yin H, Yang W, Hu M. miR-214 and miR-126 were associated with restoration of endothelial function in obesity after exercise and dietary intervention. Journal of Applied Biomedicine. 2018;16(1):34-9.
Daneshvar Medicine
Volume 30, Issue 6 - Serial Number 163
January and February 2023
Pages 44-56