Daneshvar Medicine

Daneshvar Medicine

Effect of resistance training and vitamin D with chitosan coating on Semaphorine-a3 and NLRP-1 genes of neural junction in quadriceps muscle of ovariectomized rats

Document Type : Original Article

Authors
Tahereh Shadpour Alizadeh 1
Mohammad Ali Azarbayjani 1
Sirvan Atashak 2
Maghsoud Peeri 1
Saleh Rahmati Ahmad Abad 3
1 Department of Exercise Physiology , Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Exercise Physiology , Mahabad Branch, Islamic Azad University, Mahabad, Iran
3 Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran
10.22070/daneshmed.2023.16937.1289
Abstract
Background and Objective: The menopausal process causes degeneration of motor nerve neurons in fast-twitch fibers. However, the effect of resistance training with vitamin D supplementation on the inhibition of genes involved in atrophy is not well understood. The purpose of this study was to determine the effect of resistance training and vitamin D with chitosan coating on Semaphorine-a3 and NLRP-1genes of neural junction in quadriceps muscle of postmenopausal rats.
Materials and Methods: In this experimental study, 42 femal of wistare rats with ages 8 to 12 weeks with an average weight of 230 to 260 g were randomly divided into 6 groups of 7 numbers; normal control (NC), menopause control (OC), estrogen (E), resistance training (RT),vitamin D white chitosan (Vit D+Ca+2), resistance training+Vitamin D with chitosan (RT+Vit D+Ca+2). Training group performed resistance training five days a week for eight weeks. Vitamin D with chitosan was gavage at a dose of 100 mg/kg before exercise. PCR Real time was used to determine the expression of Semaphorine-a3 and NLRP-1genes.
Results: The gene of Semaphorine-a3 in the resistance training group+vitamin D with chitosan compared to the postmenopausal control group and the training group had a significant decrease (P=0.001) and (P=0.002), respectively.  NLRP-1 gene in the resistance training group+vitamin D with chitosan was significantly reduced compared to the training group (P=0.043).
Conclusion: The results showed that resistance training decreased the  genes expression of Semaphorine-a3 and NLRP-1. Decreased expression of NLRP-1 gene under the influence of resistance training combined with vitamin D with chitosan coating could possibly improve neuromuscular function in postmenopausal people.
Keywords
Resistance training
Vitamin D with chitosan
Menopause
Semaphorine-a3
NLRP-1
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Daneshvar Medicine
Volume 30, Issue 6 - Serial Number 163
January and February 2023
Pages 32-43