Daneshvar Medicine

Daneshvar Medicine

The effect of eight weeks of resistance training on KIF5B protein in soleus and extensor digital longus muscle tissue in aged male rats

Document Type : Original Article

Authors
Mojtaba Sadegh Ghomi 1
Majid Kashef 1
Mojtaba Salehpour 1
Fariba Khodagholi 2
1 Department of Exercise Physiology, Faculty of Sport Science , Shahid Rajaee Teacher Training University, Tehran, Iran
2 Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran
10.22070/daneshmed.2022.16869.1282
Abstract
Background and Objective: The reduction of effective motor proteins in axoplasmic transport is proposed as a possible reason for the occurrence of sarcopenia. The aim of the present study was determination of the effect of eight weeks of resistance training on amount of KIF5B protein in soleus and extensor digital longus (EDL) muscle tissue in aged male rats.
Materials and Methods: 18 Aged and young male rats were randomly divided into 3 groups: aged resistance training, aged sham exercise and young sham exercise. The aged resistance training group climbed a one-meter ladder for eight weeks, 4 days a week, with one set and 8 repetitions, with a 2-minute rest interval between repetitions. To measure the amount of KIF5B protein, ELISA method was used, to measure the relative weight of muscles, a weighing scale was used, and to test the hypotheses, one-way analysis of variance and Tukey's post hoc test were used at the level of p≤0.05.
Results: Following aging, the amount of KIF5B protein decreased significantly in soleus muscle and EDL compare to Young Sham group. Eight weeks of resistance training caused a significant increase in the amount of KIF5B protein in the soleus and EDL compared to the aged sham exercise. The Relative weight of both muscles in the resistance training group had a significant increase compared to the aged sham exercise.
Conclusion: Eight weeks of resistance training increases the amount of KIF5B protein in soleus and EDL muscles and their hypertrophy in aged male rats.
Keywords
Resistance training
KIF5B
Soleus muscle
Extensor digital longus muscle
Aged rat
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Daneshvar Medicine
Volume 30, Issue 5 - Serial Number 162
November and December 2022
Pages 79-89