The effect of eight weeks of resistance training on KIF5B protein in soleus and extensor digital longus muscle tissue in aged male rats

Document Type : Original Article

Authors

1 Department of Exercise Physiology, Faculty of Sport Science , Shahid Rajaee Teacher Training University, Tehran, Iran

2 Neuroscience Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran

Abstract

Background and Objective: The reduction of effective motor proteins in axoplasmic transport is proposed as a possible reason for the occurrence of sarcopenia. The aim of the present study was determination of the effect of eight weeks of resistance training on amount of KIF5B protein in soleus and extensor digital longus (EDL) muscle tissue in aged male rats.
Materials and Methods: 18 Aged and young male rats were randomly divided into 3 groups: aged resistance training, aged sham exercise and young sham exercise. The aged resistance training group climbed a one-meter ladder for eight weeks, 4 days a week, with one set and 8 repetitions, with a 2-minute rest interval between repetitions. To measure the amount of KIF5B protein, ELISA method was used, to measure the relative weight of muscles, a weighing scale was used, and to test the hypotheses, one-way analysis of variance and Tukey's post hoc test were used at the level of p≤0.05.
Results: Following aging, the amount of KIF5B protein decreased significantly in soleus muscle and EDL compare to Young Sham group. Eight weeks of resistance training caused a significant increase in the amount of KIF5B protein in the soleus and EDL compared to the aged sham exercise. The Relative weight of both muscles in the resistance training group had a significant increase compared to the aged sham exercise.
Conclusion: Eight weeks of resistance training increases the amount of KIF5B protein in soleus and EDL muscles and their hypertrophy in aged male rats.

Keywords

References

  1. Wiedmer P, Jung T, Castro JP, Pomatto LC, Sun PY, Davies KJ, et al. Sarcopenia–Molecular mechanisms and open questions. Ageing Research Reviews 2021;65:101200.
  2. He Y, Xie W, Li H, Jin H, Zhang Y, Li Y. Cellular Senescence in Sarcopenia: Possible Mechanisms and Therapeutic Potential. Frontiers in Cell and Developmental Biology 2022;9: 793088.
  3. Larsson L, Degens H, Li M, Salviati L, Lee YI, Thompson W, et al. Sarcopenia: aging-related loss of muscle mass and function. Physiological Reviews 2019;99(1):427-511.
  4. Sleigh JN. Axonal Transport: The Delivery System Keeping Nerve Cells Alive. Frontiers for Young Minds 2020;8.
  5. Guillaud L, El-Agamy SE, Otsuki M, Terenzio M. Anterograde axonal transport in neuronal homeostasis and disease. Frontiers in Molecular Neuroscience 2020:13:556175.
  6. Sleigh JN, Rossor AM, Fellows AD, Tosolini AP, Schiavo G. Axonal transport and neurological disease. Nature Reviews Neurology 2019;15(12):691-703.
  7. Maday S, Twelvetrees AE, Moughamian AJ, Holzbaur EL. Axonal transport: cargo-specific mechanisms of motility and regulation. Neuron 2014;84(2):292-309.
  8. Cockburn JJ, Hesketh SJ, Mulhair P, Thomsen M, O'Connell MJ, Way M. Insights into kinesin-1 activation from the crystal structure of KLC2 bound to JIP3. Structure 2018;26(11):1486-1498. e6.
  9. Mahdavi Jafari, Z., Shojaie, N. Effects of Six Weeks of Spinal Nerve Ligation on Sciatic Nerve Keynesin-1 Gene Expression in Male Rats. Journal of Applied Health Studies in Sport Physiology 2018; 5(2): 28-35.
  10. Kazemi A, Rahmati M, Nezhadzamani A. Chronic effect of decreased activity in the form of spinal nerve ligation on KIF1B gene expression in male Wistar rat sciatic nerve fiber.Scientific Journal of Kurdistan University of Medical Sciences 2015; 20 (3) :23-32
  11. Gan H, Xue W, Gao Y, Zhu G, Chan D, Cheah KSE Huang J. KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes. Cell Bioscience 2019.9(85):1-16.
  12. Sato T, Ishikawa M, Mochizuki M, Ohta M, Ohkura M, Nakai J, et al. JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death & Differentiation 2015;22(8):1260-1274.
  13. Riuzzi F, Sorci G, Arcuri C, Giambanco I, Bellezza I, Minelli A, et al. Cellular and molecular mechanisms of sarcopenia: the S100B perspective. Journal of Cachexia, Sarcopenia and Muscle 2018; 9(7):1255-1268.
  14. Rahmati M, Taherabadi SJ. The effects of exercise training on Kinesin and GAP-43 expression in skeletal muscle fibers of STZ-induced diabetic rats. Scientific Reports of Nature 2021.11(9535):1-9.
  15. Kerendi H, Mirnasoori R, Rahmati M, Kazemi A. The Effect of 6 Weeks of High Intensity Interval Training on the Gene Expression and Content of KIF5B and Dynein Proteins in Hippocampus of Male Wistar Rats. Sport Physiology & Management Investigations 2018; 10(3): 123-137.
  16. Golbar SJ, Gharekhanlu R, Kordi MR, Khazani A. Effects of Endurance Exercise Training on Kinesin-5 and dynein motor proteins in sciatic nerves of male wistar rats with diabetic neuropathy. International Journal of Sport Study Health 2018.1(1):1-6:e67758.
  17. Lee S, Barton ER, Sweeney HL, Farrar RP. Viral expression of insulin-like growth factor-I enhances muscle hypertrophy in resistance-trained rats. Journal of Applied Physiology 2004;96(3):1097-104.
  18. Roberts MD, Young KC, Fox CD, Vann CG, Roberson PA, Osburn SC, and etal. An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteinsJournal of Molecular Biology and Methods 2020.24(7):1-9.e127.
  19. Rahmati M, Gharakhanlou R, Movahedin M, Mowla SJ, Khazani A, Fouladvand M, Jahani Golbar S. Treadmill training modifies KIF5B motor protein in the STZ-induced diabetic rat spinal cord and sciatic nerve. The Archives of Iranian Medicine2015.18(2):94-101.
  20. Pour M-AB, Joukar S, Hovanloo F, Najafipour H. Long-term low-intensity endurance exercise along with blood-flow restriction improves muscle mass and neuromuscular junction compartments in old rats. Iranian Journal of Medical Sciences 2017.42(6):569-576.
  21. Takenaka T, Kawakami T, Hori H, Hashimoto Y, Hiruma H, Kusakabe T. Axoplasmic transport and its signal transduction mechanism. Japanese Journal of Physiology 1998.48(6):413-420.
  22. Mahdavi Jafari Z, Shojaie N. Effects of Six Weeks of Spinal Nerve Ligation on Sciatic Nerve Keynesin-1 Gene Expression in Male Rats. Journal of Applied Health Studies in Sport Physiology 2018; 5(2): 28-35.
  23. Rahmati M, Taherabadi SJ. The effects of exercise training on Kinesin and GAP-43 expression in skeletal muscle fibers of STZ-induced diabetic rats. Scientific Reports of Nature 2021;11(9535):1-9.
  24. Bahreinipour MA, Joukar S, Hovanloo F, Najafipour H, Naderi V, Rajiamirhasani A, et al. Mild aerobic training with blood flow restriction increases the hypertrophy index and MuSK in both slow and fast muscles of old rats: Role of PGC-1α. Life Sciences 2018 1;202:103-109.
  25. Yoo S-Z, No M-H, Heo J-W, Park D-H, Kang J-H, Kim SH, et al. Role of exercise in age-related sarcopenia. Journal of exercise rehabilitation. 2018;14(4):551.
Daneshvar Medicine
Volume 30, Issue 5 - Serial Number 162
November and December 2022
Pages 79-89

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