Daneshvar Medicine

Daneshvar Medicine

The effect of eight weeks of interval training on plasma levels of sirtuin 1 and sirtuin‌ 3 proteins in inactive elderly men

Document Type : Original Article

Authors
Raana Amiri 1
Fatemeh Makipour 1
mehdi bostani 2
1 Department of Physical Education, Islamic Azad University Ahvaz Branch, Ahvaz, Iran
2 Department of Physical Education, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
10.22070/daneshmed.2022.16097.1201
Abstract
Background and Objective: SIRT proteins are involved in the body's metabolic processes and homeostasis and are associated with oxidative stress due to aging and age-related diseases. Changes in these proteins in aging and the effect of physical activity on them have been less studied, so the aim of the present study was to investigate the effect of eight weeks of interval training on plasma levels of sirt1 and sirt3 proteins in inactive elderly men.
Materials and Methods: In this study, 20 inactive elderly men aged 70-60 years were selected by available sampling and divided into experimental and control groups by simple random sampling. To investigate the effect of aging on plasma levels of Sirt1 and Sirt3 proteins, 10 inactive young men were selected by purposive sampling. The training program consisted of eight weeks of interval training with a moderate intensity of 50% of the reserve heart rate based on the principle of overload. Plasma levels of Sirt1 and Sirt3 proteins were measured in two stages of pre-test and post-test. One-way analysis of variance (ANOVA) was used to compare the pre-test of the three groups and the analysis of covariance was used to compare the post-test of the two groups of training elderly and control elderly.
Results: The results showed that plasma levels of Sirt1 and Sirt3 proteins in the elderly group were significantly lower than the young group (P <0.05). Also, due to eight weeks of interval training, the levels of the above proteins increased significantly (P <0.05).
Conclusion: According to the results of this study, it seems that  moderate interval exercise with increasing the Sirt1 and Sirt3 proteins, can reduce the oxidative stress caused by aging and lead to increased antioxidant defense and improve the quality of life in the elderly.
Keywords
Interval Training
Aging
Sirtuin 1
Sirtuin 3
  1. Johnson S, Imai S I. NAD+ biosynthesis, aging, and disease. F1000Research 2018;7 (132):1-10.
  2. Wheeler H E, Kim S K. Genetics and genomics of human ageing. Philosophical Transactions of the Royal Society B: Biological Sciences 2011;366(1561):43-50.
  3. Khosravan S, Alaviani M, Alami A, Tavakolizadeh J. Epidemiology of loneliness in elderly women. Journal of Research and Health 2014;4(4):871-877.
  4. Brenmoehl J, Hoeflich A. Dual control of mitochondrial biogenesis by sirtuin 1 and sirtuin 3. Mitochondrion 2013;13(6):755-761.
  5. Ungvari Z, Sonntag W E, Csiszar A. Mitochondria and aging in the vascular system. Journal of Molecular Medicine 2010;88(10):1021-1027.
  6. Sun N, Youle R J, Finkel T. The mitochondrial basis of aging. Molecular Cell 2016;61(5): 654-666.
  7. Guarente L, Mostoslavsky R, Kazantsev A. Introductory Review on Sirtuins in Biology, Aging, and Disease 2018; eBook ISBN: 9780128135006:1st Edition.
  8. Kong X, Wang R, Xue Y, Liu X, Zhang H, Chen Y, Fang F, Chang Y.  Sirtuin 3, a new target of PGC-1α, plays an important role in the suppression of ROS and mitochondrial biogenesis. PloS one 2010;5(7):11707-11.
  9. Yu J, Auwerx J. The role of sirtuins in the control of metabolic homeostasis. Annals of the New York Academy of Sciences 2009;1173(1):10-19.
  10. Horigome C, Oma Y, Konishi T, Schmid R, Marcomini I, Hauer M H, Dion V, Harata M, Gasser S M. SWR1 and INO80 chromatin remodelers contribute to DNA double-strand break perinuclear anchorage site choice. Molecular cell 2014;55(4):626-639.
  11. Ahn B H, Kim H S, Song S, Lee I H, Liu J, Vassilopoulos A, Deng  CX, Finkel T. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proceedings of the National Academy of Sciences 2008;105(38):14447-52.
  12. Schug T T, Li X. Sirtuin 1 in lipid metabolism and obesity. Annals of Medicine 2011;43(3):198-211.
  13. Çakir I, Perello M, Lansari O, Messier NJ, Vaslet CA, Nillni EA. Hypothalamic Sirt1 regulates food intake in a rodent model system. PloS one 2009;4(12):8322-8331.
  14. Li X. SIRT1 and energy metabolism. Acta Biochimica et Biophysica Sinica 2013;45(1):51-60.
  15. Jing E, Emanuelli B, Hirschey M D, Boucher J, Lee K Y, Lombard D, Verdin E M, Kahn C R. Sirtuin-3 (Sirt3) regulates skeletal muscle metabolism and insulin signaling via altered mitochondrial oxidation and reactive oxygen species production. Proceedings of The National Academy of Sciences 2011;108(35):14608-14613.
  16. Sharma D R, Sunkaria A, Wani W Y, Sharma RK,Verma D, Priyanka K, Bal A, Gill K D. Quercetin protects against aluminium induced oxidative stress and promotes mitochondrial biogenesis via activation of the PGC-1α signaling pathway. Neurotoxicology 2015;51:116-137.
  17. Müller G. Microvesicles/exosomes as potential novel biomarkers of metabolic diseases. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy 2012;5(1):247-255.
  18. Xu Y, Xing Y, Chen Y, Strack S, Jeffrey PD, Shi Y. Structure of the protein phosphatase 2A holoenzyme. Cell 2006;127(6):1239-1251.
  19. Li K, Lv G, Pan L. Sirt1 alleviates LPS induced inflammation of periodontal ligament fibroblasts via downregulation of TLR4. International Journal of Biological Macromolecules 2018; 119:249-254.
  20. Bakhtiyari A, Gaeni A, Chobineh S, Kordi M R. Effect of 12-weeks high-intensity interval training on SIRT1, PGC-1α and ERRα protein expression in aged rats. Journal of Applied Health Studies in Sport Physiology 2018;5(2):95-102.
  21. Billat LV. Interval training for performance: a scientific and empirical practice. Sports Medicine 2001;31(1):13-31.
  22. Huang C C, Wang T, Tung Y T, Lin W T. Effect of exercise training on skeletal muscle SIRT1 and PGC-1α expression levels in rats of different age. International Journal of Medical Sciences 2016;13(4):260-270.
  23. Fathi I, Nourshahi M, Haghparast A, Fallah H. Effect of eight-week aerobic continuous and high intensity interval training on levels of SIRT3 in skeletal muscle tissue of Wistar rats. Physiology of Sport and Physical Activity 2015; 8(2):1277-1289.
  24. Alavi S S, Joukar S, Rostamzadeh F, Najafipour H, Darvishzadeh-mahani F, Mortezaeizade A. Involvement of sirtuins and klotho in cardioprotective effects of exercise training against waterpipe tobacco smoking-induced heart dysfunction. Frontiers in Physiology 2021;12(1):894-905.
  25. Vargas-Ortiz K, Pérez-Vázquez V, Macías-Cervantes M H. Exercise and sirtuins: a way to mitochondrial health in skeletal muscle. International Journal of Molecular Sciences 2019;20(11):2717-2727.
  26. Hayes L D, Herbert P, Sculthorpe N. High intensity interval training (HIIT) produces small improvements in fasting glucose, insulin, and insulin resistance in sedentary older men but not masters athletes. Experimental Gerontology 2020;140:111074.
  27. Radak Z, Suzuki K, Posa A, Petrovszky Z, Koltai E, Boldogh I. The systemic role of SIRT1 in exercise mediated adaptation. Redox Biology 2020;35:101467.
  28. Hayes L D, Herbert P, Sculthorpe N. High intensity interval training (HIIT) produces small improvements in fasting glucose, insulin, and insulin resistance in sedentary older men but not masters athletes. Experimental Gerontology 2020;140:111074-81.
  29. Mariani S, Giorgio R D, Rossi E, Tozzi R. Blood SIRT1 Shows a Coherent Association with Leptin and Adiponectin in Relation to the Degree and Distribution of Adiposity: A Study in Obesity, Normal Weight and Anorexia Nervosa. Nutrients 2020;12(11):3506-15.
  30. Aadr arhami H, Nourshahi M, Ebrahim KH, Mousavi Mozafar M, Hedayati M. The Effect of 8-week Sprint Interval Training with Consuming Saffron Extract on the Factors Affecting Longevity in Male Rats. Iranian Journal of Nutrition Sciences & Food Technology 2019;14(1):19-26.
  31. Kincaid B, Bossy-Wetzel E. Forever young: SIRT3 a shield against mitochondrial meltdown, aging, and neurodegeneration. Frontiers in Aging Neuroscience 2013;5:48-56.
  32. Palacios O M, Carmona J J, Michan S, Chen KY, Manabe Y, Ward J L, Goodyear L J, Tong Q. Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle. Aging 2009;1(9):771-78.
  33. Vargas-Ortiz K, Perez-Vazquez V, Dia F J. Aerobic training increases expression levels of SIRT3 and PGC-1α in skeletal muscle of overweight adolescents without change in caloric intake. Pediatric Exercise Science 2015;27(2):177-184.
  34. Suwa M, Nakano H, Radak Z, Kumagai S. Endurance exercise increases the SIRT1 and peroxisome proliferator-activated receptor γ coactivator-1α protein expressions in rat skeletal muscle. Metabolism 2008;57(7):986-998.
  35. Marfe G, Tafani M, Pucci B, Stefano C D, IndelicatoM. RETRACTION: The effect of marathon on mRNA expression of anti-apoptotic and pro-apoptotic proteins and sirtuins family in male recreational long-distance runners. BMC Physiology 2013;13:13.
  36. Soleimani A, Shakerian S, Ranjbar R, Soleimani M. Effect of aerobic exhaustion with six weeks low calorie diet with moderate carbohydrate on lactate dehydrogenase and liver enzymes in overweight boys. Journal of Applied Health Studies in Sport Physiology 2017; 4(1):54-59.
  37. Chen D, Bruno J, Easlon E, Lin S J, Cheng H L, Alt F W, Guarente L. Tissue-specific regulation of SIRT1 by calorie restriction. Genes & development 2008;22(13):1753-1757.
  38. Gibala M J, McGee SL, Garnham A P, Howlett K F, Snow R J, Hargreaves M. Brief intense interval exercise activates AMPK and p38 MAPK signaling and increases the expression of PGC-1α in human skeletal muscle. Journal of Applied Physiology 2009;106(3):929-934.
  39. Hodge T, Starnes J, Feger B, Hixson L, Brennan Harris M. Effects of exercise and body temperature on eNOS, SIRT1, SIRT3 and Hsp70 expression in rat plantaris muscles (1164.6). The FASEB Journal 2014;28:1164-76.
  40. Shi T, Wang F, Stieren E, Tong Q. SIRT3, a mitochondrial sirtuin deacetylase, regulates mitochondrial function and thermogenesis in brown adipocytes. Journal of Biological Chemistry 2005;280(14):13560-13567.
  41. Sundaresan N R, Samant S A, Pillai V B, Rajamohan S B, Gupta M P. SIRT3 is a stress-responsive deacetylase in cardiomyocytes that protects cells from stress-mediated cell death by deacetylation of Ku70. Molecular and Cellular Biology 2008;28(20):6384-6401.
  42. Hokari F, Kawasaki E, Sakai A, Koshinaka K, Sakuma K, Kawanaka K. Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles. Journal of Applied Physiology 2010;109(2):332-340.
 
 

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Daneshvar Medicine
Volume 30, Issue 4 - Serial Number 161
November and December 2022
Pages 62-73