The effect of diosgenin on mitochondrial health and neutrophil infiltration indices in methotrexate-induced liver damage in the rat

Document Type : Original Article

Authors

1 Department of Physiology, School of Medicine, Shahed University, Tehran, Iran

2 Neurophysiology Research Center, Shahed University, Tehran, Iran

3 Department of Biology, School of Basic Sciences, Shahed University, Tehran, Iran

Abstract

Background and Objective: The liver is the largest gland in the body with multiple functions. Methotrexate causes acute and chronic liver damage. In this study, the effects of diosgenin were investigated as a steroidal sapogenin on mitochondrial membrane potential (MMP) and neutrophil infiltration activity in a methotrexate-induced liver injury model in male rats.
Materials and Methods: 40 rats in 5 groups; control, control treated with diosgenin 50 mg/kg, methotrexate at a dose of 20 mg/kg (i.p) and two groups treated with diosgenin at doses 10, 50 mg/kg (gavage) were divided. The rats were anesthetized and the liver tissue was isolated after killing. After tissue homogeneity, neutrophil infiltration activity and mitochondrial membrane potential were measured. For statistical analysis, one-way ANOVA and Tukey post-test were used, and the significance level was p <0.05.
Results: The potential of the mitochondrial membrane in the methotrexate group treated with diosgenin 10 mg/kg was significantly reduced compared to the control group and in the group treated with diosgenin 50 mg/kg was significantly increased compared to methotrexate. On the other hand, myeloperoxidase increased significantly in the methotrexate group and treated with diosgenin 10 mg/kg compared to the control group and decreased significantly in the group treated with diosgenin 50 mg/kg compared to the methotrexate group.
Conclusion: Diosgenin at a dose of 50 mg/kg in the methotrexate-induced liver injury model was able to improve mitochondrial health and reduced neutrophil infiltration.

Keywords

References

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Daneshvar Medicine
Volume 30, Issue 3 - Serial Number 160
September and October 2022
Pages 62-71

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