Daneshvar Medicine

Daneshvar Medicine

The effect of Sargassum tenerrimum seaweed extracts on VEGF gene expression in SW742 colorectal cancer cell line

Document Type : Original Article

Authors
Mehrnoush Masoudi 1
Hoda Khaledi 2
Bita Archangi 1
Ali Khodadadi 3
Gholamabbas Dinarvand 4
Seyedeh Maryam Mousavi 5
1 Department of Biology, Faculty of Marine Science and Oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
2 Institute of Marine Sciences, Iranian National Institute for Oceanography and Atmospheric Science, Tehran
3 Department of Immunology, Faculty of Medicine, Jondishapur University of Medical Sciences, Ahvaz, Iran
4 Department of Basic Sciences, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran
5 Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
10.22070/daneshmed.2022.15747.1169
Abstract
Background and Objective: Colon cancer is one of the most common cancers that causes many deaths worldwide. Efforts to find compounds with anti-cancer potential, especially from marine environments due to the richness of these environments in the field of organisms producing bioactive compounds, are among the topics of interest to researchers. Accordingly, the aim of this study was to investigate the effect of Sargassum tenerrimum seaweed extract on the expression of vascular endothelial growth factor (VEGF) gene in colorectal cancer cells.
Materials and Methods: In the present study, three methanolic, chloroform and hydroalcoholic extracts of Sargassum tenerrimum seaweed from Persian Gulf were prepared and the effect of three concentrations 250, 500 and 750 mg/ml of each extract on VEGF expression was evaluated in SW742 cells of colorectal cancer using Real Time PCR. Data were analyzed using One Way ANOVA along with LSD test.
Results: The results showed that hydroalcoholic extracts at concentrations of 500 and 750 mg/ml and chloroform and methanolic extracts at concentrations of 700 mg/ml caused a significant decrease in VEGF expression in SW742 cells (P <0.05).
Conclusion: These results indicate the anti-cancer potential of Sargassum tenerrimum extract by reducing VEGF expression and angiogenesis.
Keywords
Colorectal cancer
Sargassum
Angiogenesis inhibitors
Vascular endothelial growth factors
Subjects
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Daneshvar Medicine
Volume 30, Issue 3 - Serial Number 160
September and October 2022
Pages 52-61